The application discloses a non-human mammal model constructing kit with conditional gene knock-down of TCTP genes, a targeting vector, a constructing method of the targeting vector, a non-human mammal TCTP gene whole-body gene knock-down animal model, serums, an egg cell, a fertilized egg, an embryo, an offspring, tissues or cells of the animal model, application of products and the method in immunology research, research of immunotoxicology, immune rejection response and mechanism thereof, research of a cancer cell apoptosis mechanism, reach of a cancer target and cancer targeted therapy, safety evaluation and pharmacodynamic evaluation, production of a tumor pathologic model and preparation of drugs for preparing diseases based on a recombinase recognition system. The stably-inherited TCTP gene is constructed by using a homologous recombination technology and an embryonic stem cell technology for whole-body knock-down of a mouse, so that embryonic lethality caused by whole-body knock-down of the gene is avoided; the invention has important significance for researching occurrence and development of cancer and cancer treatment of the TCTP gene.