The invention provides a preparation method of an IL-6 reorganization monoclonal antibody. The preparation method comprises the following steps of performing immunization on a mouse with IL-6, after immunization, taking the spleen of the mouse, and preparing an unicellular suspension; screening memory B cells and pulp cells with a flow cytometry instrument; respectively culturing the obtained cells, adding the obtained culture supernatant in a culture device coated with the IL-6, continuing performing culturing, detecting cell strains secreting a specific antibody, and screening the antibody which can specially recognize antigen protein, and performing cloning; extracting total RNA on the obtained cells strains, performing reverse transcription to obtain cDNA, amplifying the variable regions of a heavy chain and a light chain of the antibody, and performing separate connection to a carrier; and then performing cell transfection, and producing the IL-6 reorganization monoclonal antibody. Through screening once, a large quantity of antibody sequences can be obtained, the gene information of the antibodies can be obtained, and the problems that cells in a hybridoma technique is low infusion rate and the antibody gene is lost, are solved.