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Bifidobacterium bifidum lyophilization composite protection agent and application method thereof

A bifidobacterium bifidum and protective agent technology, which is applied in the field of bifidobacteria product production, can solve the problems of easy water absorption, low number of viable bacteria, low freeze-drying survival rate, etc., and achieve the effect of improving health care function

Inactive Publication Date: 2012-02-22
SHAANXI RES INST OF AGRI PRODS PROCESSING TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The object of the present invention is to provide a kind of freeze-drying compound protective agent and application method thereof that can significantly improve the freeze-drying survival rate of Bifidobacterium bifidum and the live bacterial count of bacterial powder, solve the problem of Bifidobacterium bifidum bacterial powder in the freeze-drying process. The problems such as low freeze-drying survival rate, low number of viable bacteria, easy to absorb water and become damp, etc. have improved the health care function of the bacteria powder.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Accurately weigh 3.5g xylooligosaccharide, 6.5g inulin, 5.5g stachyose and 0.4g sodium bicarbonate, add to 80mL distilled water, heat to dissolve and mix well, then sterilize at 110°C for 15min, and cool to room temperature. Then accurately weigh 2.8g of glycine, 2.2g of arginine and 1.2g of sodium ascorbate and dissolve them in 20ml of 0.85% sterile saline, then use a 0.22μm sterile filter membrane to filter and sterilize, and finally put them in an ultra-clean workbench. The operation is to mix the two evenly to obtain the freeze-dried composite protective agent.

[0022] After Bifidobacterium bifidum was activated three times, it was inserted into the MRS medium containing 0.5g / L cysteine ​​hydrochloride by 3% (V / V) and cultivated for 18h, then 7000r / min Centrifuge for 10 minutes to obtain Bifidobacterium bifidum sludge, and adjust the viable count of Bifidobacterium bifidum sludge to 2.0×10 with 0.85% sterile saline 11 cfu / mL, add the above-mentioned freeze-dried c...

Embodiment 2

[0024] Accurately weigh 5.0g xylooligosaccharide, 8.0g inulin, 6.0g stachyose and 0.5g sodium bicarbonate, add to 80mL distilled water, heat to dissolve and mix well, then sterilize at 110°C for 15min, and cool to room temperature. Then accurately weigh 3.0g of glycine, 4.0g of arginine and 0.8g of sodium ascorbate and dissolve them in 20ml of 0.85% sterile saline, then use a 0.22μm sterile filter membrane to filter and sterilize, and finally sterilize in the ultra-clean workbench The operation is to mix the two evenly to obtain the freeze-dried composite protective agent.

[0025] After Bifidobacterium bifidum was activated three times, it was inserted into the MRS medium containing 0.5g / L cysteine ​​hydrochloride by 3% (V / V) and cultivated for 18h, then 7000r / min Centrifuge for 10 min to obtain Bifidobacterium bifidum sludge, and adjust the viable count of Bifidobacterium bifidum sludge to 1.2×10 with 0.85% sterile saline 11 cfu / mL, add the above-mentioned freeze-dried comp...

Embodiment 3

[0027] Accurately weigh 3.0g xylooligosaccharide, 6.0g inulin, 7.0g stachyose and 0.6g sodium bicarbonate, add to 80mL distilled water, heat to dissolve and mix well, then sterilize at 100°C for 20min, and cool to room temperature. Then accurately weigh 2.0g of glycine, 2.0g of arginine and 0.7g of sodium ascorbate and dissolve them in 20ml of 0.85% sterile saline, then use a 0.22μm sterile filter membrane to filter and sterilize, and finally put them in an ultra-clean workbench. The operation is to mix the two evenly to obtain the freeze-dried composite protective agent.

[0028]After Bifidobacterium bifidum was activated three times, it was inserted into the MRS medium containing 0.5g / L cysteine ​​hydrochloride by 3% (V / V) and cultivated for 18h, then 7000r / min Centrifuge for 10 minutes to obtain Bifidobacterium bifidum sludge, and adjust the viable count of Bifidobacterium bifidum sludge to 1.0×10 with 0.85% sterile saline 11 cfu / mL, add the above-mentioned freeze-dried co...

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Abstract

The invention provides a bifidobacterium bifidum lyophilization composite protection agent and application method thereof. A formula of the bifidobacterium bifidum lyophilization composite protection agent is composed of xylooligosaccharide of 3.0 to 5.0 g, synanthrin of 6.0 to 8.0 g, stachyose of 5.0 to 7.0 g, glycine 2.0 to 4.0 g, arginine of 2.0 to 4.0 g, sodium bicarbonate of 0.4 to 0.6 g andsodium ascorbate of 0.7 to1.2 g. the composite protection agent is utilized to lyophilize bifidobacterium bifidum, lyophilization survival reaches more than 85%, and viable count of bacterium powder is larger than 2.0 * 1011 cfu / g. Prebiotic of the xylooligosaccharide, synanthrin and stachyose is added in the composite protection agent so as to further improving a health protection function of the bacterium powder of the bifidobacterium bifidum. The composite protection agent resolves the important key technology problems in processes of develop high activity bacterium powder of the bifidobacterium bifidum. The prepared high activity bacterium powder can serve as addition agents and leavening agents to be used for developing functionality food and pharmaceutical preparation of the bifidobacterium bifidum.

Description

technical field [0001] The invention belongs to the technical field of production of bifidobacterium products, and relates to a freeze-drying compound protectant for improving the freeze-drying survival rate of bifidobacteria and an application method thereof. Background technique [0002] Bifidobacterium is a gram-positive, anaerobic, non-motile, non-spore-forming bacillus with irregular staining, catalase-negative, and variable morphology. Various shapes such as F or G shape, the colony is smooth, convex, and the edge is complete. About 30 species have been discovered so far, including Bifidobacterium bifidum (B.bifidum), Bifidobacterium infantis (B.infantis), Bifidobacterium adolescentis (B.adolescentis), Bifidobacterium longum (B.longum) And Bifidobacterium breve (B.breve) and so on. [0003] Bifidobacterium is one of the probiotics commonly used in dairy products and their preparations. It can relieve lactose intolerance and irritable bowel syndrome, prevent diarrhea ...

Claims

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Application Information

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IPC IPC(8): C12N1/04C12R1/01
Inventor 舒国伟胡曼陈合马齐秦涛张华吴玉喜
Owner SHAANXI RES INST OF AGRI PRODS PROCESSING TECH
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