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Method for preparing high-purity stachyose by using plant chromatographic separation technology

A technology of industrial chromatography and separation technology, applied in the field of stachyose preparation, which can solve the problems of low purity of polysaccharides, limited audience and application fields, low functionality, etc., achieve high yield, increase farmers' income, and reduce losses low rate effect

Active Publication Date: 2010-01-27
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purity of its functional oligosaccharides is not high, which limits the audience and application fields

Method used

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  • Method for preparing high-purity stachyose by using plant chromatographic separation technology
  • Method for preparing high-purity stachyose by using plant chromatographic separation technology

Examples

Experimental program
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Effect test

Embodiment 1

[0033] (1) Juicing: choose 10kg of non-mildew hay caddis, wash it, add 40L of water at 80°C for 1 hour, extract the residue and add water twice the weight of the residue at 80°C for 1 hour, and mix. The juice squeezed twice is used as sugar solution;

[0034] (2) Impurity removal: heat up the sugar solution to 90° C., heat for 20 minutes, filter, remove the precipitate, add clarifier sodium hydroxide to the filtrate at a ratio of 6 g: 1 L, let stand for precipitation for 60 minutes, and take supernatant;

[0035] (3) Decolorization: according to the ratio of 1L: 6g, add activated carbon to the supernatant, heat to 90°C, decolorize for 40 minutes, and filter to remove the activated carbon;

[0036] (4) Desalination: the decolorized liquid is cooled to 25°C, and the conductivity is reduced to below 50 μs / cm through cation exchange resin, anion exchange resin, cation exchange resin, and anion exchange resin in sequence;

[0037] (5) Membrane filtration: filter the desalted liqu...

Embodiment 2

[0042] A method for preparing high-purity stachyose with industrial chromatographic separation technology, comprising the steps of:

[0043] (1) Juicing: According to the ratio of mass ratio of 1:4, add water to the dry caddis worm and extract at 70°C for 1.0 hour, squeeze the juice, take the residue, add water twice the mass of the residue and extract at 70°C for 1.0 hour Re-squeezing, mixing the squeezed juice twice to make sugar liquid;

[0044] (2) Impurity removal: the sugar solution was heated to 80° C., heated for 20 minutes, filtered, and the precipitate was removed. The clarifier sodium hydroxide was added to the filtrate at a ratio of 8 g: 1 L, and allowed to settle for 60 minutes. supernatant;

[0045] (3) According to the ratio of 1L: 7g, add activated carbon to the supernatant, heat to 80°C, decolorize for 50 minutes, and filter to remove the activated carbon;

[0046] (4) Desalination: The decolorized liquid is lowered to 20°C, and the conductivity is reduced t...

Embodiment 3

[0052] A method for preparing high-purity stachyose with industrial chromatographic separation technology, comprising the steps of:

[0053] (1) Juicing: According to the ratio of mass ratio of 1:3, add water to the dry silver white strips and extract at 50°C for 1.5 hours, squeeze the juice, take the residue, add water 1 times the mass of the residue, and extract at 50°C for 1.5 hours. Squeeze, mix twice squeezed juice to be sugar liquid;

[0054] (2) Impurity removal: the sugar solution was heated to 50° C., heated for 30 minutes, filtered, and the precipitate was removed. The clarifying agent sodium hydroxide was added to the filtrate at a ratio of 5 g: 1 L, and allowed to settle for 90 minutes. supernatant;

[0055] (3) Decolorization: according to the ratio of 1L: 4g, add activated carbon to the supernatant, heat to 60°C, decolorize for 60 minutes, and filter to remove the activated carbon;

[0056] (4) Desalination: The decolorized liquid is lowered to 30°C, and the co...

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Abstract

The invention discloses a method for preparing high-purity stachyose by using a plant chromatographic separation technology, comprising the following steps: (1) squeezing juice: leaching dry Chinese ortichoke or silvery strips, squeezing juice, extracting residues, leaching and re-squeezing by adding water, and mixing juice twice and taking the juice as sugar liquid; (2) removing impurities; (3) decolorizing; (4) desalting; (5) filtering by a film; (6) concentrating; (7) separating a chromatogram: selecting porous gel resin as an industrial chromatogram fixing phrase, and separating sugar content in Brix sugar liquid of 40-60 DEG C by using a size exclusion principle; (8) concentrating and drying spray to obtain high-purity stachyose. The method applies a size exclusion chromatographic technology to purify functional oligosaccharide and can successfully obtain the high-purity stachyose from natural plants and provide a technical platform for purifying other kinds of functional oligosaccharide; the separated purity is higher than 90 percent (a radio of stachyose to total sugar), and the stachyose has higher yield and lower loss ratio.

Description

technical field [0001] The invention relates to a method for preparing stachyose, in particular to a method for preparing high-purity stachyose by using industrial chromatographic separation technology. Background technique [0002] Stachyose is a functional oligosaccharide that naturally exists in the Lamiaceae stachyus plant and can significantly promote the proliferation of beneficial bacteria in the human intestinal tract. It is known as "natural super strong bifidus factor". Stachyose has a clear structure, molecular formula C 24 h 42 o 21 , with a molecular weight of 666.59, it is composed of fructose, glucose, galactose, and galactose, and its simple structure is expressed as fructose-glucose-galactose-galactose. [0003] Stachyose product is a new product developed in the past 10 years, it is a kind of sugar mixture with stachyose as the main component. The technology of extracting stachyose products from raw materials such as caddis worms is mature, and the stac...

Claims

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Application Information

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IPC IPC(8): C07H3/06C07H1/08B01D15/08
Inventor 张金泽周志桥曾明林静刘炎桥
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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