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Method for producing lactic acid by using glycerol

A technology of glycerin and lactic acid, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of reducing production costs, saving costs, and broadening the range of choices

Inactive Publication Date: 2008-09-03
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been no reports of lactic acid fermentation using raw materials other than sugary raw materials

Method used

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  • Method for producing lactic acid by using glycerol
  • Method for producing lactic acid by using glycerol

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0015] (1) Fermentation method: 5L mechanical stirring fermentation tank, batch fermentation with air

[0016] (2) Bacteria: Escherichia coli

[0017] (3) Culture medium: glycerol produced by chemical method

[0018]

[0019] (4) Fermentation process:

[0020] A. Seed culture: Escherichia coli was inserted into the seed medium containing 20g / L glycerol (250ml Erlenmeyer flask, liquid volume 100ml), the culture temperature was 37°C, the shaker speed was 150rpm, and aerobic culture was carried out for 24h.

[0021] B. Fermentation culture: a 5L fermenter was used for fermentation culture, the liquid volume was 4L, the culture temperature was 37°C, and the pH value was controlled by sodium hydroxide to 6.5. The seed liquid was inserted into the initial fermentation medium containing 50g / L glycerol according to the inoculum amount of 5%. During the fermentation process, glycerin was not added, and microaerobic fermentation was adopted, 0.5vvm air was introduced, and the rotat...

example 2

[0025] (1) Fermentation method: 500mL triangular flask, shaker micro-aerobic fermentation

[0026] (2) Strains: Enterococcus faecalis

[0027] (3) Culture medium: glycerol produced by chemical method,

[0028]

[0029] (4) Fermentation process:

[0030] A. Seed culture: Inoculate Enterococcus faecalis into a seed medium containing 20g / L glycerol (250ml Erlenmeyer flask, liquid volume: 100ml), culture at 30°C, shaker speed 150rpm, aerobic culture for 24h.

[0031] B. Fermentation culture: insert the seed liquid into the initial fermentation medium containing 30g / L glycerol according to the inoculation amount of 1%, in a 500ml Erlenmeyer flask, with a liquid volume of 200ml, at a culture temperature of 37°C, at a shaker speed of 150rpm, and aerobic fermentation for 72 hours .

[0032] (5) Fermentation result:

[0033] At the end of 72hr fermentation, the concentration of lactic acid in the fermentation broth was 13.65g / l. The mass yield of lactic acid is 0.58, and the pr...

example 3

[0035] (1) Fermentation method: 5L mechanical stirring fermentation tank, air flow and batch fermentation

[0036] (2) Bacteria: Klebsiella

[0037] (3) Culture medium: Glycerol is the by-product glycerol obtained in the biodiesel production process, and a part of it is used as a fermentation substrate with an initial concentration of 30 g / l, and the other part is used for fed-batch feeding. Other ingredients are the same as Example 1

[0038] (4) Fermentation process:

[0039] A. Seed culture: insert Klebsiella into the seed medium containing 20g / L biodiesel by-product glycerin (250ml Erlenmeyer flask, liquid volume 100ml), culture temperature 37 ℃, shaker speed 150rpm, aerobic Cultivate for 24h.

[0040] B. Fermentation culture: a 5L fermenter was used for the fermentation culture, the liquid volume was 4L, the culture temperature was 41°C, and the pH value was controlled to 6.0 with sodium hydroxide. The seed liquid was inserted into the initial fermentation medium cont...

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Abstract

The invention is a method for producing lactic acid for a substrate by glycerin, belonging to the field of biochemical technology. The method comprises the steps that: strains containing glycerin use genes and lactic acid synthetic genes are inoculated to a glycerin-containing seed culture medium; seed liquid is added to an initial fermentation medium containing 30-80g / L glycerin for anaerobic fermentation or micro-aerobic fermentation under 37 DEG C to 55 DEG C; the pH value is adjusted by fed batch 3-4M aqueous slkali or portion-wise addition of solid CaCO<3>, when the production strength of the lactic acid is less than 0.3-0.5g / (1h); the glycerin is stopped fed batching, the fermentation is continued until the residual glycerin concentration decreased to 5g / L. The method has the advantages that: the by product glycerin in the biodiesel production process is reasonably used, and transferred to high-added-value lactic acid; the method can also be used for coupling production of biodiesel and lactic acid, by which high-added-value biodiesel and lactic acid are produced with low-cost raw material, the utilization rate of raw material is improved, and the production cost is lowered.

Description

technical field [0001] The invention belongs to the field of biochemical technology, and particularly provides a method for producing lactic acid using glycerol as a substrate. Background technique [0002] Lactic acid, whose scientific name is α-hydroxypropionic acid, can be divided into D-type, L-type and DL-type according to its optical activity. Lactic acid is an important organic acid that can be widely used in food, pharmaceuticals, leather, textiles, cigarettes, printing and dyeing and other industrial fields. Its main products are sour agents, flavoring agents, preservatives, tanning agents, plant growth regulator Agents and chiral drugs, etc. In addition, lactic acid is still being vigorously developed in the field of application, the production of polylactic acid (PLA), polylactic acid is non-toxic, non-irritating, and has good biocompatibility. It is a biodegradable and absorbable , high-strength, and plastically processed polymer materials. The practical medical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/56C12R1/22C12R1/19C12R1/01
Inventor 刘德华孙燕欧先金
Owner TSINGHUA UNIV
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