Electrochemical DNA biosensor for detecting BCR/ABL fusion gene of chronic myeloid leukemia (CML)

A chronic granulocyte and biosensor technology, applied in the direction of electrochemical variables of materials, determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of long detection period, low sensitivity and high price, and achieve short detection period. , the effect of high sensitivity

Inactive Publication Date: 2010-12-29
林新华 +4
View PDF0 Cites 19 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the embodiments of the present invention is to provide an electrochemical DNA sensor for detecting the BCR/A...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Electrochemical DNA biosensor for detecting BCR/ABL fusion gene of chronic myeloid leukemia (CML)
  • Electrochemical DNA biosensor for detecting BCR/ABL fusion gene of chronic myeloid leukemia (CML)
  • Electrochemical DNA biosensor for detecting BCR/ABL fusion gene of chronic myeloid leukemia (CML)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention clearer, the present invention will be further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0021] The electrochemical DNA sensor for detecting the BCR / ABL fusion gene of chronic myeloid leukemia provided by the embodiment of the present invention, the BCR / ABL fusion gene to be detected comprises the following steps:

[0022] (1) Find the fusion site of the BCR / ABL fusion gene from the NCBI gene database, select the bases before and after the site as artificially synthesized probes, and select the gene segment with the highest mutation rate in the BCL-ABL gene as a probe , after homologous comparison, the corresponding sequence was synthesized as the specific sequence of CML (5'-N...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides an electrochemical DNA biosensor for detecting the BCR/ABL fusion gene of chronic myeloid leukemia (CML), which comprises the following steps of: (1) designing and synthesizing the specificity sequence of the CML according to the fusion locus of the universal primer sequence of the selected CML gene type to be detected; and (2) building the electrochemical DNA biosensor by combining a chemical bonding technology, a molecular hybridization technology, a locked nucleic acid probe technology with an electrochemical enzyme linked immunity technology for detecting the BCR/ABL fusion gene of the CML. The biosensor greatly strengthens the sensitivity and the specificity, thereby being capable of realizing the early diagnosis of the chronic myeloid leukemia (CML).

Description

technical field [0001] The invention relates to an electrochemical DNA biosensor for detecting chronic myelogenous leukemia BCR / ABL fusion gene Background technique [0002] Chronic myelocytic leukemia (CML) is a cell line disease caused by malignant lesions at the level of pluripotent stem cells with acquired chromosomal abnormalities, and its clinical features are significant granulocyte overproduction . Chronic myeloid leukemia is one of the malignant blood diseases that seriously endanger the health of young and middle-aged people, and it is a more common type of leukemia. Because leukemia patients do not have obvious symptoms in the early stage of the disease, it brings a certain degree of difficulty to the early diagnosis and detection of leukemia, and the level of early diagnosis will directly affect the treatment effect and quality of life of leukemia patients, its significance cannot be overstated And metaphor. [0003] At present, the clinical diagnosis methods ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68G01N27/26
Inventor 林新华刘爱林陈敬华王昆陈伟
Owner 林新华
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap