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Electrochemical DNA biosensor for detecting BCR/ABL fusion gene of chronic myeloid leukemia (CML)

A chronic granulocyte and biosensor technology, applied in the direction of electrochemical variables of materials, determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of long detection period, low sensitivity and high price, and achieve short detection period. , the effect of high sensitivity

Inactive Publication Date: 2010-12-29
林新华 +4
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Problems solved by technology

[0008] The technical problem to be solved by the embodiments of the present invention is to provide an electrochemical DNA sensor for detecting the BCR / ABL fusion gene of chronic myeloid leukemia, aiming to solve the problems of low sensitivity, long detection period and high price of existing detection methods.

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  • Electrochemical DNA biosensor for detecting BCR/ABL fusion gene of chronic myeloid leukemia (CML)
  • Electrochemical DNA biosensor for detecting BCR/ABL fusion gene of chronic myeloid leukemia (CML)
  • Electrochemical DNA biosensor for detecting BCR/ABL fusion gene of chronic myeloid leukemia (CML)

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Embodiment Construction

[0020] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention clearer, the present invention will be further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0021] The electrochemical DNA sensor for detecting the BCR / ABL fusion gene of chronic myeloid leukemia provided by the embodiment of the present invention, the BCR / ABL fusion gene to be detected comprises the following steps:

[0022] (1) Find the fusion site of the BCR / ABL fusion gene from the NCBI gene database, select the bases before and after the site as artificially synthesized probes, and select the gene segment with the highest mutation rate in the BCL-ABL gene as a probe , after homologous comparison, the corresponding sequence was synthesized as the specific sequence of CML (5'-N...

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Abstract

The invention provides an electrochemical DNA biosensor for detecting the BCR / ABL fusion gene of chronic myeloid leukemia (CML), which comprises the following steps of: (1) designing and synthesizing the specificity sequence of the CML according to the fusion locus of the universal primer sequence of the selected CML gene type to be detected; and (2) building the electrochemical DNA biosensor by combining a chemical bonding technology, a molecular hybridization technology, a locked nucleic acid probe technology with an electrochemical enzyme linked immunity technology for detecting the BCR / ABL fusion gene of the CML. The biosensor greatly strengthens the sensitivity and the specificity, thereby being capable of realizing the early diagnosis of the chronic myeloid leukemia (CML).

Description

technical field [0001] The invention relates to an electrochemical DNA biosensor for detecting chronic myelogenous leukemia BCR / ABL fusion gene Background technique [0002] Chronic myelocytic leukemia (CML) is a cell line disease caused by malignant lesions at the level of pluripotent stem cells with acquired chromosomal abnormalities, and its clinical features are significant granulocyte overproduction . Chronic myeloid leukemia is one of the malignant blood diseases that seriously endanger the health of young and middle-aged people, and it is a more common type of leukemia. Because leukemia patients do not have obvious symptoms in the early stage of the disease, it brings a certain degree of difficulty to the early diagnosis and detection of leukemia, and the level of early diagnosis will directly affect the treatment effect and quality of life of leukemia patients, its significance cannot be overstated And metaphor. [0003] At present, the clinical diagnosis methods ...

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Application Information

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IPC IPC(8): C12Q1/68G01N27/26
Inventor 林新华刘爱林陈敬华王昆陈伟
Owner 林新华
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