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Surface plasma resonance probe with silver-gold core-satellite structure and preparation method thereof

A technology of surface plasmon and satellite structure, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of high recurrence rate of lung cancer, easy metastasis, and low concentration of effective drugs in tumor sites, and achieve obvious blue shift phenomenon and high detection sensitivity Effect

Inactive Publication Date: 2013-09-18
NANJING UNIV OF POSTS & TELECOMM
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Problems solved by technology

At present, the treatment methods for lung cancer are limited to drug therapy, radiotherapy and clinical surgery. These conventional treatment methods have non-specific distribution of anticancer drugs in the patient's body, low effective drug concentration at the tumor site, and limited monitoring methods for treatment effects. And the delivery efficiency of drugs to the target site is poor, and the recurrence rate after resection of advanced lung cancer is high, and it is easy to transfer. Therefore, it is imminent to develop a highly sensitive and effective method for early detection of high-risk groups of tumors.

Method used

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  • Surface plasma resonance probe with silver-gold core-satellite structure and preparation method thereof
  • Surface plasma resonance probe with silver-gold core-satellite structure and preparation method thereof
  • Surface plasma resonance probe with silver-gold core-satellite structure and preparation method thereof

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Embodiment 1

[0027] Embodiment 1~6 each group distribution ratio and performance parameter are as table 1:

[0028] Table 1 Each performance parameter of embodiment 1~6

[0029]

[0030] It can be seen from Table 1 that the surface plasmon resonance scattering spectra of all examples combined with miRNA-21 (1 uM) have obvious blue shift phenomenon, and the spectrum of Example 5 has a blue shift of 30 at the binding amount of 1 ul. nm, indicating that the surface plasmon resonance probe still has excellent detection performance in the presence of trace markers, and the detection sensitivity reaches 10 nM.

[0031] Embodiment 1~6 adopts identical preparation method, and concrete steps are as follows:

[0032] 1) Preparation of nano-silver cubes

[0033] First, 3-8 mM glucose solution and 50-80 mM hexadecyltrimethylammonium bromide solution were quickly added to quantitative silver ammonia solution 10-15 mM at room temperature. The concentration ratio of alkyltrimethylammonium bromide s...

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Abstract

The invention discloses a surface plasma resonance probe with a silver-gold core-satellite structure and a preparation method thereof. The preparation method comprises the steps of: firstly, sequentially and quickly adding a glucose solution and a hexadecyl trimethyl ammonium bromide solution into a quantitative silver ammonia solution for reacting to prepare a nano silver cube solution; and then, standing a clean glass substrate in the nano silver cube solution to prepare a nano silver cube glass substrate; fixing single stranded DNA molecules on the prepared nano silver cube glass substrate; hybridizing the single stranded DNA with a miRNA molecule; and finally, preparing gold nanoparticles and decorating the gold nanoparticles at one end of the miRNA molecule to prepare the surface plasma resonance probe with a silver-gold core-satellite structure and capable of being applied in early detection of lung cancer. The particle diameter of the prepared surface plasma resonance probe is 50-70nm, the scattered spectrum peak is located at 450-650nm and the detection sensitivity is as high as 10nM.

Description

technical field [0001] The invention relates to a surface plasmon resonance probe, in particular to a silver-gold "core-satellite" structure surface plasmon resonance probe for early detection of lung cancer and a preparation method thereof. Background technique [0002] Malignant tumors are a major killer of human beings. Lung cancer is the most common primary malignant tumor of the lung, and it is currently the number one cause of cancer death in the world. At present, the treatment methods for lung cancer are limited to drug therapy, radiotherapy and clinical surgery. These conventional treatment methods have non-specific distribution of anticancer drugs in the patient's body, low effective drug concentration at the tumor site, and limited monitoring methods for treatment effects. And the delivery efficiency of the drug to the target site is poor, and the recurrence rate of advanced lung cancer after resection is high, and it is easy to transfer. Therefore, it is imminent...

Claims

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Application Information

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IPC IPC(8): G01N21/25
Inventor 张磊汪联辉张颖黄维
Owner NANJING UNIV OF POSTS & TELECOMM
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