Composite gene chip and method for detection of fourteen common pathogenic bacteria

A technology of gene chip and pathogenic bacteria, applied in the field of compound gene chip for detecting fourteen common pathogenic bacteria

Inactive Publication Date: 2013-01-02
冯家望 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the large variety of microbial toxins and the existence of many derivative compounds with similar structures, the existing methods have great limitations in terms of specificity, sensitivity, scope of application, detection cost, etc.

Method used

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  • Composite gene chip and method for detection of fourteen common pathogenic bacteria
  • Composite gene chip and method for detection of fourteen common pathogenic bacteria
  • Composite gene chip and method for detection of fourteen common pathogenic bacteria

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Embodiment Construction

[0109]The present invention is aimed at Staphylococcus aureus, Salmonella, Campylobacter jejuni, Yersinia enterocolitica, Vibrio parahaemolyticus, Vibrio cholerae, enterohemorrhagic Escherichia coli O157:H7, Bacillus cereus , Clostridium perfringens, Shigella, Listeria monocytogenes, Clostridium botulinum, Aspergillus flavus, and Aspergillus parasiticus to design a composite gene chip and provide Method for chip detection.

[0110] 1. Design of target gene probes and primers

[0111] Through gene analysis of the above-mentioned fourteen kinds of common pathogenic bacteria, the specific identification target genes of the above-mentioned fourteen kinds of common pathogenic bacteria were obtained, and corresponding primers and probes were designed for these target genes. See Table 1 for relevant information, and Table 2 for primers and target gene information.

[0112] Table 1 Information about oligonucleotide probes for detecting fourteen common pathogenic bacteria

[0113] ...

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Abstract

The invention relates to a gene chip and a method; aiming at specific identification target genes of fourteen common pathogenic bacteria, the invention designs a corresponding primer and probe, and constructs a composite gene chip; the primer is marked by biotin, then is used for amplification of a target gene fragment of the substance to be detected by a PCR method; the amplification product is hybridized with the probe on the composite gene chip; through dual specific detection of the primer and the probe, the compound deposits on the composite gene surface, and specific molecules is hybridized to be converted into a visual signal; therefore, various toxigenic microorganisms can be detected. The invention can detect 14 common pathogenic bacteria, is high in detection flux, strong in specificity, good in sensitivity, rapid and effective.

Description

【Technical field】 [0001] The invention relates to a gene chip, in particular to a composite gene chip for detecting 14 kinds of common pathogenic bacteria and a detection method using the composite gene chip. 【Background technique】 [0002] Biotoxins, also known as natural toxins, refer to toxic chemical substances from biological sources that cannot be replicated independently, including animal toxins, plant toxins, and microbial toxins. Among them, microbial toxins are mainly secreted during the growth of toxin-producing microorganisms and released from the body after death, and can be divided into bacterial toxins and mycotoxins. Common bacterial toxins include Staphylococcus aureus toxin, enterotoxigenic Escherichia coli enterotoxin, Shiga-like toxin, Shiga toxin, cholera toxin, hemolytic toxin, etc. Common mycotoxins include aflatoxins and fumonisins. Rapid and accurate detection of many of the above-mentioned microbial toxins is the premise and key to effectively pre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C40B40/06C12R1/445C12R1/42C12R1/63C12R1/19C12R1/145C12R1/085C12R1/67C12R1/66C12R1/01
CPCY02A50/30
Inventor 冯家望王小玉邝筱珊游淑珠胡松楠成晓维唐食明
Owner 冯家望
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