Composite gene chip and method for detection of fourteen common pathogenic bacteria

Abstract

The invention relates to a gene chip and a method; aiming at specific identification target genes of fourteen common pathogenic bacteria, the invention designs a corresponding primer and probe, and constructs a composite gene chip; the primer is marked by biotin, then is used for amplification of a target gene fragment of the substance to be detected by a PCR method; the amplification product is hybridized with the probe on the composite gene chip; through dual specific detection of the primer and the probe, the compound deposits on the composite gene surface, and specific molecules is hybridized to be converted into a visual signal; therefore, various toxigenic microorganisms can be detected. The invention can detect 14 common pathogenic bacteria, is high in detection flux, strong in specificity, good in sensitivity, rapid and effective.

Description

【Technical field】 [0001] The invention relates to a gene chip, in particular to a composite gene chip for detecting 14 kinds of common pathogenic bacteria and a detection method using the composite gene chip. 【Background technique】 [0002] Biotoxins, also known as natural toxins, refer to toxic chemical substances from biological sources that cannot be replicated independently, including animal toxins, plant toxins, and microbial toxins. Among them, microbial toxins are mainly secreted during the growth of toxin-producing microorganisms and released from the body after death, and can be divided into bacterial toxins and mycotoxins. Common bacterial toxins include Staphylococcus aureus toxin, enterotoxigenic Escherichia coli enterotoxin, Shiga-like toxin, Shiga toxin, cholera toxin, hemolytic toxin, etc. Common mycotoxins include aflatoxins and fumonisins. Rapid and accurate detection of many of the above-mentioned microbial toxins is the premise and key to effectively pre...

AI Technical Summary

Problems solved by technology

Due to the large variety of microbial toxins and the existence of many derivative compounds with similar structures, the existing methods have great limitations in terms of specificity, sensitivity, scope of application, detection cost, etc.

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