Preparation method and application of electrochemical sensor capable of simultaneously detecting two acute leukemia markers

An acute leukemia and marker technology, applied in the field of clinical analysis and bioelectrochemical sensing, can solve the problems of low accuracy and difficult judgment, reduce detection cost, save detection time, and promote early diagnosis and typing. effect of development

Active Publication Date: 2014-07-23
QINGDAO UNIV
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Problems solved by technology

Traditional diagnostic methods mostly rely on the changes in tumor tissue or cell morphology, but the judgment is difficult and the accuracy rate is low

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  • Preparation method and application of electrochemical sensor capable of simultaneously detecting two acute leukemia markers

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Embodiment Construction

[0028] With reference to the accompanying drawings, a method for preparing an electrochemical sensor for simultaneous detection of two acute leukemia markers comprises the following steps:

[0029] (1) Add 1 μmol / L lysozyme reporter probe (5'-mercapto-C6-GTGCAGAGCTAAGTAACTCTGCAC-methylene blue-3') and 1 μmol / L γ-interferon reporter probe (5'-mercapto-C6-TTCAACCAACTTTTTTTTTTCAACACCCAT-ferrocene -3') in 50 μL solution containing 0.5 μL 10 mmol / L tris(2-chloroethyl) phosphate (TCEP) (20 mmol / L Tris-HCl, pH7.4, 140 mmol / L NaCl, 20 mmol / L MgCl2, 20 mmol / L L KCl) at room temperature for 2 h to open the disulfide bond. Then add 1 μmol / L lysozyme aptamer (5'-ATC AGG GCT AAA GAG TGC AGA GTT ACT TAG-3') and 1 μmol / L interferon aptamer (5'-GGG GTT GGT TGT GTT GGG TGT TGT GT-3'), the lysozyme reporter probe and the lysozyme aptamer form a lysozyme reporter probe-aptamer double-stranded structure, and the γ-interferon reporter probe and the γ-interferon aptamer form a γ-interferon reporte...

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Abstract

The invention discloses a preparation method of an electrochemical sensor capable of simultaneously detecting two acute leukemia markers. The method comprises the following steps: respectively standing a lysozyme report probe and a gamma-interferon report probe in a liquid containing trichloroethyl phosphate (TCEP), so as to open a disulfide bond and respectively form a double-chain structure together with a lysozyme aptamer and a gamma-interferon aptamer; preprocessing a gold electrode, and immersing the processed gold electrode into the pre-processed mixed liquid of lysozyme report probe-aptamer double chains and gamma-interferon report probe-aptamer double chains; standing at room temperature over the night, and then cleaning by using secondary distilled water and a cleanout fluid; immersing the electrode into the liquid containing MCH to seal the electrode, and then cleaning the secondary distilled water and the cleanout fluid; and taking the electrode processed by the above steps as a work electrode to be connected on a chemical work station together with a reference electrode and a counter electrode, so as to obtain the electrochemical sensor. The electrochemical sensor can be applied to simultaneous detection of two acute leukemia markers, namely lysozyme and gamma-interferon.

Description

technical field [0001] The invention relates to a preparation method and application of an electrochemical sensor for simultaneously detecting two acute leukemia markers, belonging to the technical fields of clinical analysis and bioelectrochemical sensing. Background technique [0002] In today's society, cancer is one of the diseases that seriously threaten human health. The main reason for the high mortality rate of cancer patients is that early diagnosis cannot be carried out. If early diagnosis and timely treatment are available, 1 / 3 of cancers can be cured. The determination of serum tumor markers plays an important role in the early diagnosis of cancer. Therefore, the establishment of an accurate and rapid analytical method and corresponding equipment for the detection of tumor markers in serum has attracted great attention. [0003] Acute leukemia is the most common malignant tumor in children and young people. It is extremely harmful to children's health. Its earl...

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Application Information

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IPC IPC(8): G01N27/26G01N27/327
Inventor 王宗花赵凯夏建飞宋岱珉张菲菲夏霖迟德玲李延辉夏延致夏临华
Owner QINGDAO UNIV
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