The present invention provides a method of avoiding reusing a test strip which is used for measuring the analyte or the indicator like glucose in physiological liquid like blood, tissue fluid or urine. The present invention also relates to the method of avoiding reusing the test strip which comprises an integrated lancet or a skin puncture device like a needle, a blade or other edge tools. Some kinds of medical devices, such as a glucose test strip, are expected to be used for one test and then are disposed. Because a plurality of chemical reagents in the test strip are not fit for measuring the glucose for second time, the requirement is needed frequently. Some users possibly test the used test strips accidentally. If a glucose meter regulator is used for trying to measure the glucose and outputting a result, which can possibly become the potential problem, therefore, a required one-off test strip and a meter regulator are provided with the appointed method used for avoiding reusing the tested test strip.