The invention belongs to the field of nuclear magnetic resonance imaging data processing, and relates to a movie nuclear magnetic resonance image sequence motion field estimation method based on a fractional order differential. The method aims to solve the problems that the method of enhancing and directly building a light stream equation through integer order differential images in the prior art is used for estimating a movie nuclear magnetic resonance image sequence motion field, grain details of the images are lost, an estimation result is influenced by illumination changes, the rotation unchanging performance does not exist, the noise resisting performance is poor, and precision based on movie nuclear magnetic resonance image sequence motion field estimation is low. The method mainly comprises the steps that firstly, grain enhancing is carried out on a movie nuclear magnetic resonance image through the fractional order differential; secondly, monogenic signals of the image are extracted through Riesz transformation, namely, the monogenic phase, the monogenic direction and the monogenic amplitude; thirdly, the light stream equation is built through the phase vector of the monogenic signals; fourthly, the movie nuclear magnetic resonance image sequence motion field is estimated through the light stream equation. The method is used for estimating the motion of an imaging object by the movie nuclear magnetic resonance image.