Method for screening probiotic combination based on multiple pathogenic bacteria of prawn diseases

Abstract

The invention discloses a method for screening a probiotic combination based on multiple pathogenic bacteria of prawn diseases. The method is characterized by comprising the following steps: respectively acquiring intestinal bacterial community composition and abundance information of both healthy prawns at different time nodes and ill prawns at corresponding time points, and removing prawn age indication strains; screening bacterial groups which are not planted in the intestinal tracts of the prawns in an initial healthy state, are linearly increased along with the deterioration of prawn diseases and have abundance significantly higher than the abundance of healthy prawns at corresponding time points, defining the bacterial groups as multi-pathogenic bacteria, and determining the varieties of the multi-pathogenic bacteria; screening out strains with delta B smaller than -0.5 and an ASCH-score greater than 1.5 as direct antagonistic strains of multi-pathogenic bacteria, and defining the strains as probiotics; and taking a ratio of the relative abundance of a single probiotic to the sum of the relative abundance of the probiotics as the individual number percentage of the corresponding probiotics, and finally determining the combination and proportions of the probiotics. The method has the advantages of higher accuracy and better stability.

Description

technical field [0001] The invention relates to a method for screening probiotics for aquaculture animals, in particular to a method for screening a combination of probiotics based on the multi-pathogenic bacteria of prawn diseases. Background technique [0002] With the continuous development of my country's shrimp aquaculture industry, the aquaculture mode has changed from earth pond aquaculture to intensive, high-density greenhouse and recirculating aquaculture. This transformation leads to the increase of stressors and the decline of shrimp immunity, which makes the problem of shrimp farming diseases increasingly prominent. Shrimp diseases have the characteristics of rapid onset and high mortality, but currently there is no particularly effective treatment method. The loss caused by shrimp diseases in my country is as high as tens of billions of yuan every year. Antibiotics are still widely used in the prevention and treatment of shrimp diseases. The abuse of antibiot...

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