Similarity and intermiscibility principle for guiding microorganism compatibility and compatibility method for improving function and efficiency of exogenously added microorganisms
A technology of microbial strains and microbial colonies, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of ignoring the antagonism of the functional relationship of exogenous microorganisms
Active Publication Date: 2019-11-26
PEKING UNIV
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AI Technical Summary
Problems solved by technology
At present, the commonly used compatibility method is to randomly combine microbial strains. The screening and combination of strains is a completely random process, ignoring the functional relationship between exogenous microorganisms and the possible differences between them and endogenous microorganisms in the target environment. Antagonism
Method used
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Experimental program
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Effect test
Embodiment 1
[0057] Embodiment 1, establishment of "similar and compatible" compatibility method
[0058] 1. Analysis of community structure in the target environment and screening of main dominant species
[0059] The analysis method flow is as follows figure 1 shown.
[0060] 1. Select the oil-contaminated soil in the oil sludge tank of Shengli Oilfield, after pretreatment (remove surface debris and then scoop up the soil above 5cm), put the soil in a sterile bag and store it in a 4°C incubator to the laboratory, extract Soil Genomic DNA.
[0061] 2. Using the soil genomic DNA obtained in step 1 as a template, perform PCR amplification to obtain archaeal colony 16S rDNA and bacterial colony 16S rDNA respectively. The amplification system and thermal cycle program of archaeal 16S rDNA are shown in Table 1. The bacterial 16S rDNA amplification system and thermal cycle program are shown in Table 2.
[0062] Table 1 Amplification system and thermal cycle program of archaeal 16S rDNA
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Abstract
The invention discloses a similarity and intermiscibility principle for guiding microorganism compatibility, and a compatibility method for improving function and efficiency of exogenously added microorganisms. The similarity and intermiscibility compatibility method provided by the invention comprises the following steps: firstly, analyzing a community structure in a target environment, and screening out main dominant communities in the community structure; screening out bacterial groups with obviously increased relative abundance, namely competitive dominance, by combining with an analysis result of biological activation; and finally, with a 16SrDNA sequence as a reference, screening a plurality of strains which simultaneously have stronger functions and are similar to target indigenousdominant communities or activated competitive dominant communities in phylogenetic development from a strain resource library for compatibility, and then culturing, mixing and adding the microorganisms into a target environment. The method is of great significance to petroleum pollution treatment.
Description
technical field [0001] The invention relates to a "similar and compatible" principle guiding microbial compatibility and a compatibility method for improving the function and efficiency of exogenously added microorganisms. Background technique [0002] Microorganisms do not exist as individuals in nature, but in the form of communities. Natural environment, including soil environment (coexistence of animals and microorganisms), water environment (coexistence of animals, plants and microorganisms) and reservoir environment (the oil layer environment itself is very complex, and crude oil itself is also composed of alkanes, aromatic hydrocarbons, non-hydrocarbons and asphaltenes Composition of the mixture) and so on have complex and diverse characteristics. It is difficult for a single species of microorganisms to adapt to these environments. At the same time, due to the strong preference of microorganisms for the selection of carbon sources, it is difficult for a single micr...
Claims
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Patent Timeline
Login to View More IPC IPC(8): C12N1/20B09C1/10C12R1/01C12R1/05
CPCB09C1/10C12N1/20
Inventor吴晓磊汤岳琴聂勇王健伟
OwnerPEKING UNIV