Bacterial strain and flocculating agent for bacterial fermentative solution pretreatment as well as preparation methods and application methods of bacterial strain and flocculating agent

A technology of bacterial fermentation and application methods, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve problems such as difficult and efficient separation, high energy consumption, and increased cost, and achieve simple and huge extraction and preparation methods Promote the application value and the effect of simple fermentation process

Active Publication Date: 2013-09-04
CENT SOUTH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, for smaller bacteria and fermented liquid with higher viscosity, it is difficult to achieve efficient separation by conventional separation methods, and the cost is often increased due to high energy consumption

Method used

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  • Bacterial strain and flocculating agent for bacterial fermentative solution pretreatment as well as preparation methods and application methods of bacterial strain and flocculating agent
  • Bacterial strain and flocculating agent for bacterial fermentative solution pretreatment as well as preparation methods and application methods of bacterial strain and flocculating agent

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Embodiment 1

[0021] 1) Preparation of strains

[0022] Pannonibacter phragmitus B7 (CCTCCNO: M2013025) strain was sampled and isolated from the activated sludge of printing and dyeing wastewater plants. After 72 hours of culture on LB medium, it formed light yellow and viscous colonies. Through physiological and biochemical experiments, it was shown that the bacteria were Gram-negative bacteria. The bacterium can secrete and express extracellular polymers, and the polymers can be used as flocculants in the pretreatment of fermentation broth. So far, there is no report on the use of P.phragmitetus strains to obtain microbial flocculants. The bacterium has been preserved in the China Center for Type Culture Collection, the preservation number is: CCTCCNO:M2013025, and the preservation address is: Wuhan University, Wuhan, China.

[0023] 2) Extraction and preparation of microbial flocculant

[0024] The composition formula (g / L) of fermentation medium is: glucose 20-40, (NH 4 ) 2 SO 4 0...

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Abstract

The invention provides a bacterial strain and a flocculating agent for bacterial fermentative solution pretreatment as well as preparation methods and application methods of the bacterial strain and the flocculating agent. The flocculating agent is prepared by fermenting, culturing and extracting a microbial bacterial strain Pannonibacter phragmitetus B7 (CCTCC NO: M2013025), and then is used for bacterial fermentative solution pretreatment. The fermentation process of the microbial flocculating agent is simple; the flocculating agent is mainly secreted outside cells; and the extraction and preparation method of the flocculating agent is simple, low in cost and suitable for mass production. The acting pH value of the microbial flocculating agent is 2-13; and the flocculating agent is suitable for the flocculating and settling pretreatment of the bacterial fermentative solution and has an enormous popularization and application value.

Description

technical field [0001] The present invention relates to novel microorganisms, flocculant, preparation method and application method thereof for the pretreatment of bacterial fermentation broth, specifically refers to the use of microbial flocculant to produce bacteria Pannonibacter phragmitetus B7 (it has been preserved in the China Typical Culture Collection Center, and the preservation number is: CCTCCNO: M2013025) produces flocculants and uses the microbial flocculants for the treatment of bacterial fermentation broth. Background technique [0002] After the fermentation is finished, the fermentation liquid not only contains the target product, but also contains a large amount of bacteria and various metabolites. Usually, it is necessary to separate the bacteria and suspended solids from the soluble components first. For larger cells and suspended particles, conventional filtration or centrifugation methods can be used. However, for smaller bacteria and fermented liquid...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04C12R1/125C12R1/19C12R1/01
Inventor周洪波田健郑甲王玉光
OwnerCENT SOUTH UNIV