Phytobacterium plantarum MPs-68 capable of adsorbing micro-plastics and degrading uric acid and application of phytobacterium plantarum MPs-68

Abstract

The invention relates to the technical field of microorganisms, in particular to plant lactobacillus MPs-68 capable of adsorbing microplastics and degrading uric acid and application of the plant lactobacillus MPs-68. The provided plant lactobacillus MPs-68 capable of adsorbing microplastics and degrading uric acid is a marine-derived natural strain, the preservation number is CGMCC No.31976, the plant lactobacillus MPs-68 has a remarkable adsorption effect on microplastics polystyrene and polyethylene, has the function of degrading uric acid, and is high in oxidation resistance, gastric acid resistance, cholate resistance and intestinal colonization capacity, and the plant lactobacillus MPs-68 can be used for adsorbing microplastics and degrading uric acid. The method has important application value in the fields of food or health care products for assisting adsorption of micro-plastics and reduction of uric acid and the like. The invention also provides a microbial agent, a probiotic product, a functional food, a fermentation product, a microecological preparation, a microplastic adsorbent and a microplastic adsorbent compounded with the lactobacillus brevis MPA01 (the preservation number is CGMCC No.31977) containing the phytobacterium plantarum MPs-68 capable of adsorbing the microplastics and degrading the uric acid, and the microplastic adsorbent has the effects of adsorbing the microplastics, degrading the uric acid and the like.

Description

Technical Field

[0001] The present invention relates to the field of microbial technology, and in particular to a Lactobacillus plantarum MPs-68 capable of absorbing microplastics and degrading uric acid and an application thereof. Background Art

[0002] Microplastics (MPs) usually refer to tiny plastic particles with a diameter of less than 5 mm, mainly including polystyrene (PS), polyethylene (PE) and polypropylene (PP). As MPs are widely used in human daily life, MPs have entered the ocean, land and freshwater systems in various ways, and then enriched in organisms with the transmission of the food chain. Due to the poor biodegradability and insufficient recycling effect of MPs, MPs have caused extensive pollution to the environment. In addition, researchers from the National Center for Environmental Health / Agency for Toxic Substances and Disease Registry (NCEH / ATSDR) have also proposed that MPs have potential hazards to human health. Based on this, how to effectively reduce the harm of microplastic pollution to humans, animals, plants and water resources has become a hot topic of current research. Chinese patent CN117187111A discloses a strain of Lactobacillus plantarum DT77 and its application in the preparation of products with microplastic adsorption and removal functions, but the strain only has outstanding performance in adsorbing PS fluorescent microspheres, and its antioxidant capacity is mediocre. More importantly, the strain does not have the ability to degrade uric acid.

[0003] Hyperuricemia (HUA) caused by purine metabolism disorder is a common metabolic disease characterized by elevated serum uric acid (UA) levels. Hyperuricemia, hypertension, hyperglycemia, and hyperlipidemia are collectively referred to as "four high" metabolic diseases. The key to treating hyperuricemia is to inhibit xanthine oxidase and reduce uric acid concentration. In current treatment methods, in addition to drug treatment, strict dietary control is generally required to minimize the intake of exogenous purine substances. However, patients have low compliance with the treatment plan that requires strict dietary control while taking medication. Therefore, how to use lactic acid bacteria to participate in the prevention and treatment of hyperuricemia has become one of the current hot topics. Chinese patent CN115786187A discloses a plant lactobacillus that can degrade uric acid, a probiotic composition, and its application. The strain has the ability to efficiently degrade uric acid, with an in vitro degradation effect of 30%. It also has good acid resistance and good tolerance to gastrointestinal fluid, but does not have the ability to adsorb microplastics.

[0004] Therefore, finding a human-suitable strain that can both effectively adsorb MPs and degrade uric acid remains an urgent problem to be solved in the current field. Summary of the invention

[0005] In order to obtain a human-suitable strain that can effectively adsorb MPs and degrade uric acid, the present invention provides a plant lactobacillus MPs-68 that can adsorb microplastics and degrade uric acid, the Latin name is Lactiplantibacillus plantarum The deposit number is CGMCC No.31976, and it was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on September 14, 2024. The address of the deposit unit is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0006] The plant lactobacillus MPs-68 with the ability to adsorb microplastics and degrade uric acid was isolated from a sample of sweet osmanthus fish roe in the sea area of ​​Xiamen City, Fujian Province. It has a significant adsorption effect on microplastics polystyrene (PS) and polyethylene (PE), has good inhibitory ability on xanthine oxidase, has the ability to degrade uric acid, has strong antioxidant ability, has a high free radical scavenging rate for 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH), is resistant to gastric acid and bile salts, and has good intestinal colonization ability.

[0007] The present invention also provides a Lactobacillus plantarum MPs-68 bacterial agent, whose components include the Lactobacillus plantarum MPs-68 capable of adsorbing microplastics and degrading uric acid as described above.

[0008] Furthermore, the number of viable Lactobacillus plantarum MPs-68 in the Lactobacillus plantarum MPs-68 bacterial agent is ≥ 1×10 11 CFU / g or ≥1×10 11 CFU / mL.

[0009] The present invention also provides a Lactobacillus plantarum MPs-68 probiotic product, whose components include the Lactobacillus plantarum MPs-68 having the ability to adsorb microplastics and degrade uric acid as described above.

[0010] The present invention also provides a Lactobacillus plantarum MPs-68 functional food, the components of which include the Lactobacillus plantarum MPs-68 capable of absorbing microplastics and degrading uric acid as described above.

[0011] Furthermore, the functional food includes any one of microplastic adsorbing functional food, uric acid degrading functional food, antioxidant functional food, acid-resistant functional food and bile-resistant functional food, or a combination of any two or more thereof.

[0012] The present invention also provides a Lactobacillus plantarum MPs-68 fermentation product, whose components include the Lactobacillus plantarum MPs-68 capable of adsorbing microplastics and degrading uric acid as described above.

[0013] The present invention also provides a microecological preparation, the components of which include the Lactobacillus plantarum MPs-68 capable of adsorbing microplastics and degrading uric acid as described above.

[0014] The present invention also provides a microplastic adsorbent, the components of which include Lactobacillus plantarum MPs-68 or a composite bacteria of Lactobacillus plantarum MPs-68 and Lactobacillus curvatum MPA01; Wherein, the Lactobacillus plantarum MPs-68 is the Lactobacillus plantarum MPs-68 with the ability to adsorb microplastics and degrade uric acid as described above, and the Lactobacillus curvatum MPA01 is deposited with the General Microbiology Center of the China Microbiological Culture Collection Administration on September 14, 2024, and the address of the depository is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing; The number of viable bacteria of Lactobacillus plantarum MPs-68 is ≥ 1×10 9 CFU / g or ≥1×10 9 CFU / mL, the viable count of the composite bacteria is ≥1×10 9 CFU / g or ≥1×10 9 CFU / mL.

[0015] Furthermore, the present invention also provides the use of the microplastic adsorbent as described above in adsorbing polystyrene microplastics and / or polyethylene microplastics.

[0016] The Lactobacillus plantarum MPs-68 provided by the present invention has the following beneficial effects: The Lactobacillus plantarum MPs-68 with the ability to adsorb microplastics and degrade uric acid provided by the present invention is isolated from a sample of sweet osmanthus fish roe in the sea area of ​​Xiamen City, Fujian Province. It is a natural strain of marine origin and has high food safety.

[0017] The Lactobacillus plantarum MPs-68 with the ability to adsorb microplastics and degrade uric acid provided by the present invention has a significant adsorption effect on microplastics polystyrene (PS) and polyethylene (PE), and can be used to adsorb polystyrene microplastics or polyethylene microplastics or mixed microplastics of polystyrene and polyethylene, breaking through the dilemma of the single adsorption object of microplastic adsorption functional bacteria in the current field.

[0018] The Lactobacillus plantarum MPs-68 with the ability to adsorb microplastics and degrade uric acid provided by the present invention also has good inhibitory ability against xanthine oxidase and has obvious ability to degrade uric acid. It has important application value in the fields of auxiliary adsorption of microplastics and lowering uric acid, as well as in the fields of food or health products.

[0019] The Lactobacillus plantarum MPs-68 provided by the present invention that has the ability to adsorb microplastics and degrade uric acid also has a very high DPPH free radical scavenging rate and strong antioxidant capacity.

[0020] The Lactobacillus plantarum MPs-68 capable of adsorbing microplastics and degrading uric acid provided by the present invention is resistant to gastric acid and bile salts, has good tolerance to gastric juice and intestinal juice, and has good intestinal colonization ability, which is conducive to its preparation as a probiotic product.

[0021] The Lactobacillus plantarum MPs-68 bacterial agent, probiotic product, functional food, fermented product and microecological preparation provided by the present invention contain Lactobacillus plantarum MPs-68 which has the function of adsorbing microplastics and degrading uric acid. The Lactobacillus plantarum MPs-68 is a natural bacterial species of marine origin, has high food safety, is excellent in the adsorption capacity for microplastics PS and PE and the ability to degrade uric acid, has strong antioxidant capacity, is resistant to gastric acid and bile salts, and has high intestinal colonization efficiency.

[0022] The microplastic adsorbent provided by the present invention comprises components including Lactobacillus plantarum MPs-68 capable of adsorbing microplastics and degrading uric acid, or a composite bacteria including Lactobacillus plantarum MPs-68 capable of adsorbing microplastics and degrading uric acid and Lactobacillus curvatus MPA01. The Lactobacillus plantarum MPs-68 has significant adsorption effects on both microplastics PS and PE, among which the adsorption effect on microplastics PS is more significant. In addition, after being combined with Lactobacillus curvatus MPA01, the microplastic adsorption rate is further improved. In particular, the adsorption of microplastics PE has a significant synergistic effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0024] Figure 1 This is a diagram showing the adsorption effect of microplastics PS by Lactobacillus plantarum MPs-68, which has the ability to adsorb microplastics and degrade uric acid, provided in Example 1 of the present invention; Figure 2 This is a diagram showing the adsorption effect of Lactobacillus plantarum MPs-68 on microplastics PE provided in Example 1 of the present invention, which has the ability to adsorb microplastics and degrade uric acid; Figure 3 A colony morphology diagram of Lactobacillus plantarum MPs-68 capable of adsorbing microplastics and degrading uric acid provided in Example 2 of the present invention; Figure 4 This is a Gram staining display of Lactobacillus plantarum MPs-68 capable of adsorbing microplastics and degrading uric acid provided in Example 2 of the present invention; Figure 5Schematic diagram of the phylogenetic tree based on 16S rRNA sequence of Lactobacillus plantarum MPs-68 with the ability to adsorb microplastics and degrade uric acid provided in Example 2 of the present invention. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments, and the technical features designed in different implementations of the present invention described below can be combined with each other as long as they do not conflict with each other. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] The present invention provides a plant lactobacillus MPs-68 capable of absorbing microplastics and degrading uric acid, the Latin name of which is Lactiplantibacillus plantarum The deposit number is CGMCC No.31976, and it was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on September 14, 2024. The address of the deposit unit is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0027] Strain source: The Lactobacillus plantarum MPs-68 was isolated from a sweet-scented osmanthus fish roe sample in the sea area of ​​Xiamen City, Fujian Province. The DNA genome of the strain was extracted and PCR amplification was performed. The PCR amplification product was sequenced by 16S rRNA. Through Blast sequence alignment, the 16S rRNA gene sequence of the strain was highly homologous to that of Lactobacillus plantarum, and it was named Lactobacillus plantarum MPs-68.

[0028] Colony morphology: round, milky white, smooth, raised, with neat edges and opaque on MRS solid culture medium.

[0029] Physiological and biochemical characteristics of the strain: A single colony of the Lactobacillus plantarum MPs-68 was picked for Gram staining and catalase test. The Lactobacillus plantarum MPs-68 was a Gram-positive bacterium with a short rod-shaped cell shape, negative for catalase and oxidase, and no spores.

[0030] Physiological and biochemical functions of the strain: The Lactobacillus plantarum MPs-68 is a natural strain of marine origin with high food safety; it has a significant adsorption effect on microplastics polystyrene (PS) and polyethylene (PE); it has a good inhibitory effect on xanthine oxidase and has the ability to degrade uric acid; it has a high DPPH free radical scavenging rate and strong antioxidant capacity; it is resistant to gastric acid and bile salts, and has good intestinal colonization ability.

[0031] Strain isolation process: Take the sweet osmanthus fish roe sample from the sea area of ​​Xiamen, Fujian Province, place it in a sterile homogenizing bag, add 30mL of 0.85% physiological saline, and beat until completely mixed to obtain a sample solution; draw 1mL of the above sample solution for a 10-fold series of gradient dilutions, and draw 10 dilutions at a dilution factor of 10. 3 , 10 4 , 10 5 200 μL of each sample was spread on a MRS solid culture medium plate containing 2.5% CaCO3 and inverted for 48 h at 37°C; colonies with good growth and large calcium dissolution zone were picked from the culture plate and repeatedly isolated and purified by plate streak separation method until a single colony was obtained. The isolated strain was named MPs-68 and stored in a bacteria bank at -80°C in glycerol tubes; Among them, the formula of MRS solid culture medium is: 20.0 g glucose, 15.0 g agar powder, 10.0 g tryptone, 10.0 g beef extract, 5.0 g yeast extract powder, 5.0 g anhydrous sodium acetate, 2.0 g dipotassium hydrogen phosphate, 2.0 g ammonium citrate, 0.5 g magnesium sulfate, 0.25 g manganese sulfate monohydrate, 1.0 mL Tween-80, 1 L deionized water, pH = 6.5 (MRS liquid culture medium is obtained by removing 15.0 g agar powder).

[0032] Example 1 Screening of strain MPs-68 1.1 Determination of adsorption capacity of microplastics polystyrene (PS) Lactic acid bacteria isolated from marine samples and frozen in glycerol tubes were activated for two generations in the bacterial library and inoculated into MRS liquid medium at a 3% (v / v) inoculation volume. The culture was cultured at 37°C for 24 h, and the fermentation broth was centrifuged at 4°C and 5000 rpm for 10 min. The supernatant was discarded, and 500 μL of sterile PBS buffer was added to wash twice. The bacteria were resuspended in sterile PBS buffer and the concentration of the bacterial solution was adjusted to 1×10 9 CFU / mL.

[0033] Experimental group: Add 100 μL of the bacterial suspension to each 1.5 mL EP tube, then add 900 μL of PS fluorescent microsphere working solution and mix thoroughly; Blank control group: add 100 μL sterile PBS buffer to a 1.5 mL EP tube, then add 900 μL PS fluorescent microsphere working solution and mix thoroughly; The PS fluorescent microsphere working solution is monodisperse fluorescent microspheres sold by Aladdin Reagents, with a concentration of 0.16 mg / mL and a particle size of 5 μm.

[0034] The experimental group and the blank control group were placed on a shaker and incubated at 37°C, 300 rpm, and protected from light for 4 h. After the incubation, the incubation solution of the experimental group and the blank control group was taken and centrifuged at 2000 rpm for 10 min. The incubation solution was observed. Figure 1 As shown, numbered is the experimental group, numbered is the blank control group. In the blank control group, the PS fluorescent microspheres at the bottom of the EP tube did not self-aggregate, while in the experimental group, a large number of adsorbed agglomerated flocs of strain MPs-68 and PS fluorescent microspheres appeared at the bottom of the EP tube with strain MPs-68 bacterial suspension added.

[0035] 100 μL of the supernatant after centrifugation was taken into a 96-well ELISA plate, and the fluorescence intensity was measured by an ELISA instrument. The adsorption rate was calculated according to the adsorption rate calculation formula (1) based on the fluorescence intensity value. The data results are shown in Table 1. Among them, the parameters of the microplate reader are: excitation wavelength is 488 nm, detection wavelength is 518 nm; Adsorption rate calculation formula (1):

[0036] A: Fluorescence value of blank control group; B: Fluorescence values ​​of the experimental group.

[0037] Table 1 Results of determination of PS adsorption rate of microplastics

[0038] According to the data in Table 1, the strain MPs-68 extracted, separated, purified and cultured from the sweet osmanthus fish roe sample has an adsorption rate of 62.14% for microplastic PS, which is the highest microplastic PS adsorption rate compared with other strains in the experimental group.

[0039] Based on the above-mentioned macroscopic flocculation phenomenon of the incubation solution and the results of the microplastic PS adsorption rate determination, it can be determined that the strain MPs-68 has a significant adsorption effect on microplastic PS. Based on this, the strain MPs-68 was further tested for its microplastic polyethylene (PE) adsorption capacity.

[0040] 1.2 Determination of adsorption capacity of microplastic polyethylene (PE) Experimental group: Add 100 μL of the bacterial suspension to a 1.5 mL EP tube, then add 900 μL of PE fluorescent microsphere working solution and mix thoroughly; Blank control group: add 100 μL sterile PBS buffer to a 1.5 mL EP tube, then add 900 μL PE fluorescent microsphere working solution and mix thoroughly; Among them, the PE fluorescent microsphere working solution is the fluorescent microsphere working solution sold by Zhongke Keyou, with a concentration of 0.16 mg / mL and a particle size of 5 μm.

[0041] The experimental group and the blank control group were placed on a shaker at 37°C and 300 rpm for 4 h in the dark. After the incubation, the incubation solution of the experimental group and the blank control group was taken and centrifuged at 2000 rpm for 10 min. The incubation solution was observed. Figure 2 As shown, No. 1 is the experimental group, and No. 2 is the blank control group. In the blank control group, the PE fluorescent microspheres at the bottom of the EP tube did not self-aggregate, while in the experimental group, a large number of adsorbed and aggregated flocs of strain MPs-68 and PS fluorescent microspheres appeared at the bottom of the EP tube.

[0042] 100 μL of the supernatant after centrifugation was taken from each well of a 96-well ELISA plate, and the fluorescence intensity was measured using an ELISA reader. The adsorption rate was calculated according to the fluorescence intensity value according to the adsorption rate calculation formula (1). The parameters of the ELISA reader were as follows: the excitation wavelength was 488 nm, and the detection wavelength was 520 nm.

[0043] According to calculations, the adsorption rate of strain MPs-68 on microplastic PE is 48.28%. Figure 5 From the incubation liquid flocculation result diagram shown, it can be seen that the strain MPs-68 also has a significant adsorption effect on microplastic PE.

[0044] 1.3 Determination of the adsorption capacity of strain MPs-68 and strain MPA01 for microplastics PS and PE It should be noted that strain MPA01 is a Lactobacillus curvatum, also known as Latilactobacillus curvatus The deposit number is CGMCC No.31977, and it was deposited in the General Microbiology Center of China Culture Collection Administration on September 14, 2024. This strain has the ability to adsorb microplastics.

[0045] The strains MPs-68 and MPA01 were activated for two generations, inoculated into MRS liquid medium at a 3% (v / v) inoculation rate, and cultured at 37°C for 24 h. The fermentation broth was centrifuged at 4°C and 5000 rpm for 10 min, the supernatant was discarded, and 500 μL of sterile PBS buffer was added to wash twice. The bacterial precipitates of strains MPs-68 and MPA01 were resuspended in sterile PBS buffer to adjust the concentration of the composite bacterial solution to 1×10 9 CFU / mL.

[0046] The composite bacterial suspensions of strains MPs-68 and MPA01 were tested according to the above-mentioned method for determining the adsorption capacity of microplastics PS and PE. The adsorption rate was calculated according to the fluorescence intensity value according to the adsorption rate calculation formula (1). The data results are shown in Table 2: Table 2 Determination of adsorption rate of microplastics PS and PE by strain MPs-68 and strain MPA01

[0047] According to the data in Table 2, the adsorption rate of microplastic PS by the composite bacteria of strains MPs-68 and MPA01 was 66.25% ( p >0.05), the adsorption rate of PE is 52.70% ( p <0.05), compared with the adsorption rate of 62.14% and 48.28% of microplastics PS and PE by strain MPs-68 alone, there was a significant improvement, indicating that the combination of strain MPs-68 and strain MPA01 has an improving effect on the adsorption rate of microplastics PS and PE, especially, it has a significant synergistic effect on the adsorption of microplastics PE.

[0048] In summary, the strain MPs-68 provided by the present invention has an adsorption rate of 62.14% for microplastics PS and an adsorption rate of 48.28% for microplastics PE. It has significant adsorption effects on both microplastics PS and PE, among which the adsorption effect on microplastics PS is more significant. In addition, the strain MPs-68 is compounded with Lactobacillus curvatus MPA01, and the adsorption rate of the composite bacteria on microplastics PS is 66.25% ( p >0.05), the adsorption rate of microplastic PE was 52.70% ( p <0.05), it can be seen that the compounding produces a synergistic effect, among which the synergistic effect on the adsorption of microplastics PE is more significant.

[0049] Example 2 Identification of strain MPs-68 2.1 Colony morphology identification The strain MPs-68 glycerol-preserved bacteria were taken out from the bacteria library and inoculated into MRS liquid culture medium at a 2% (v / v) inoculation rate for strain activation. The strain was cultured in a shaking incubator at 37°C and 180 rpm for 24 h, and then streaked on an MRS solid culture medium plate. The plate was inverted and cultured at 37°C for 24 h to obtain a single colony.

[0050] Colony morphology reference Figure 3 As shown, the single colony of the strain MPs-68 is round, milky white, smooth and raised on the surface, with neat edges and is opaque.

[0051] 2.2 Physiological and biochemical tests The above-mentioned single colonies with good growth were selected for Gram staining and catalase test. The test results were compared with the "Berger's Manual of Systematic Bacteriology, Eighth Edition" for preliminary identification of the strain. Specifically, the Gram staining microscopy of the strain MPs-68 was referenced Figure 4As shown, Gram stain is purple and positive, the cell shape is short rod-shaped, catalase and oxidase are negative, and there is no spore formation.

[0052] 2.3 16S rRNA gene sequence identification (1) Extraction of strain DNA Take 2 mL of the strain culture medium into a centrifuge tube, centrifuge at 12000 rpm for 1 min, remove the supernatant, and retain the bacteria; Add 200 μL of 20 mg / mL lysozyme to the centrifuge tube and treat at 37°C for more than 30 min; Add 200 μL of solution A to the centrifuge tube, oscillate thoroughly or use a pipette to repeatedly blow up and down to fully suspend the bacteria, add 20 μL of 10 mg / mL RNaseA to the suspension, mix thoroughly by inversion, and place at room temperature for 15-30 min; Add 20 μL of 10 mg / mL proteinase K to the centrifuge tube, mix thoroughly, and digest at 55°C for 30-60 min. During the digestion period, invert the centrifuge tube several times to mix until the sample is completely digested to obtain a clear and viscous bacterial solution. Add 200 μL of solution B to the centrifuge tube and mix thoroughly by inverting. If a white precipitate appears during this period, place it at 75°C for 15-30 min. The precipitate will disappear and you can continue the operation. Add 200 μL of anhydrous ethanol to the centrifuge tube and mix thoroughly. Flocculent precipitation may appear during this period, which does not affect the extraction of strain DNA. Add the solution and flocculent precipitation to the adsorption column and let it stand for 2 min. Centrifuge at 12000 rpm for 2 min, discard the waste liquid, and place the adsorption column in a collection tube; Add 600 μL of rinse solution to the adsorption column, centrifuge at 12000 rpm for 1 min, discard the waste liquid, and place the adsorption column in a collection tube; Add 600 μL of rinse solution to the adsorption column again, centrifuge at 12000 rpm for 1 min, discard the waste liquid, and place the adsorption column in a collection tube; Centrifuge at 12000 rpm for 2 min, leave the adsorption column open at room temperature for 15 min, and remove the residual rinse solution in the adsorption column to prevent the residual rinse solution from affecting subsequent enzyme digestion, PCR and other experimental operations; Place the adsorption column in a clean centrifuge tube, drop 100 μL of the eluent preheated in a 65°C water bath into the center of the adsorption membrane, place at room temperature for 5 min, and centrifuge at 12,000 rpm for 1 min. The eluate obtained by centrifugation was suspended and added to the adsorption column, placed at room temperature for 2 minutes, and centrifuged at 12000 rpm for 2 minutes to obtain high-quality strain DNA.

[0053] Among them, the kit is a bacterial genomic DNA extraction kit sold by Tiangen Biochemical Technology (Beijing) Co., Ltd.; Lysozyme buffer: 20 mmol / L Tris (pH=8.0), 2 mmol / L Na2-EDTA, 1.2% TritonX-100.

[0054] (2) PCR amplification of 16S rRNA gene using strain DNA as template The strain DNA is amplified by PCR, and the PCR amplification product is sequenced by 16S rRNA; Among them, the amplification primers used were universal primers 27F (5′-AGAGTTTGATCCTGGCTCAG-3′) and 1492R (5′-TACGGCTACCTTGTTACGACTT-3′); The PCR reaction system was: DNA 2 μL, 27F 2 μL, 1492R 2 μL, Premix Ex Taq 25 μL, ddH2O 19 μL; The PCR reaction conditions were as follows: pre-denaturation at 94°C for 3 min; denaturation at 94°C for 30 s, annealing at 55°C for 30 s, and extension at 72°C for 1 min, 30 cycles; and extension at 72°C for 5 min.

[0055] The 16S rRNA sequencing company is Fuzhou Qingke Biotechnology Co., Ltd.

[0056] The sequencing results were searched for similar sequences in the NCBI database using Blast software, and the strain MPs-68 sequence was compared with the 16S rRNA gene sequence of related species obtained from the gene bank. The phylogenetic tree was constructed using Mega7.0 software. Figure 5 The gene sequence is as follows: 27F: 5'-AGAGTTTGATCCTGGCTCAG-3' (SEQ ID NO. 1); 1492R: 5'-TACGGCTACCTTGTTACGACTT-3' (SEQ ID NO. 2); 16S rRNA gene sequence determination results:

[0057] Based on the comprehensive analysis of the colony morphology, cell morphology, physiological and biochemical characteristics and 16S rRNA gene sequence identification results of the strain MPs-68, the strain MPs-68 was identified as Lactobacillus plantarum ( Lactiplantibacillus plantarum ).

[0058] Example 3 Determination of the ability of Lactobacillus plantarum MPs-68 to degrade uric acid 3.1 Determination of xanthine oxidase inhibition activity The glycerol-preserved strains of Lactobacillus plantarum MPs-68 were taken out from the bacterial library, activated for 2 generations, cultured at 37°C for 24 h, centrifuged at 12,000 rpm, the supernatant discarded, and 500 μL PBS buffer (pH = 7.0) was added to resuspend the bacteria as the sample solution to be tested.

[0059] The reaction was carried out in a PBS buffer system at pH = 7.0. The amount of reagent added for each test item is shown in Table 3: Table 3 Xanthine oxidase inhibition activity assay system configuration table

[0060] Take the test process of test item A as an example: add 125 μL of the sample solution to be tested, 100 μL of 0.1 U / mL xanthine oxidase solution and 575 μL of PBS buffer into the test tube, shake for 30 s, react at 37°C for 10 min, then add 200 μL of 2 mmol / L xanthine solution, shake for 30 s, react at 37°C for 10 min, centrifuge, take the supernatant and measure the absorbance at 295 nm, repeat 3 times.

[0061] The absorbance value of each test item at 295 nm was calculated according to the xanthine oxidase inhibition rate calculation formula (2). The data results are shown in Table 4:

[0062] Table 4 Determination of xanthine oxidase inhibition activity of Lactobacillus plantarum MPs-68

[0063] According to the data in Table 4, the inhibition rate of Lactobacillus plantarum MPs-68 on xanthine oxidase was (67.44±0.07)%, which had a significant inhibitory effect.

[0064] 3.2 Determination of uric acid degradation ability Lactobacillus plantarum MPs-68 was activated for 2 generations and inoculated into MRS liquid medium supplemented with 700 μmol / L uric acid at an inoculum volume of 3% (v / v). A blank control group was set up as MRS liquid medium containing 700 μmol / L uric acid without inoculation of the strain. The cells were cultured at 37°C for 24 h, centrifuged at 12,000 rpm, and the supernatant was used as the test solution; According to the Nanjing Jiancheng Uric Acid Detection Kit Operating System Instructions shown in Table 5, each reaction reagent was added, mixed, and the absorbance value A at 510 nm was measured. The initial uric acid content was calculated according to the uric acid concentration calculation formula (3), and the data are summarized in Table 6; After incubation at 37°C for 10 min, the absorbance value A at 510 nm was measured, and the residual uric acid content was calculated according to the uric acid concentration calculation formula (3). The data are summarized in Table 6. The degradation rate of uric acid by Lactobacillus plantarum MPs-68 was calculated according to the uric acid degradation rate calculation formula (4), and the data are summarized in Table 6; in:

[0065] A 空白 : absorbance value of blank group; A 标准 : absorbance value of standard group; A 待测 : absorbance value of the test liquid group; C 校准品 : Calibrator concentration, 400 μmol / L.

[0066]

[0067] UA 初始 : Initial uric acid concentration, μmol / L; UA 剩余 : Residual uric acid concentration, μmol / L.

[0068] Table 5 Nanjing Jiancheng Uric Acid Detection Kit Operating System Instructions

[0069] Table 6 Results of the determination of uric acid degradation ability of Lactobacillus plantarum MPs-68

[0070] According to the data in Table 6, the degradation rate of uric acid by Lactobacillus plantarum MPs-68 is 74.59%, which has a significant effect on degrading uric acid.

[0071] Example 4 Determination of Antioxidant Capacity of Lactobacillus plantarum MPs-68 Lactobacillus plantarum MPs-68 was activated for 2 generations and inoculated into MRS liquid medium at a 3% (v / v) inoculation volume. The culture was incubated at 37°C for 24 h, centrifuged at 4000 rpm for 10 min, the supernatant was discarded, 500 μL of sterile PBS buffer was added to wash twice, and then sterile PBS buffer was added to resuspend the bacteria to adjust the bacterial solution concentration to 1×10 9 CFU / mL.

[0072] Experimental group: Take 500 μL of bacterial suspension, add 500 μL of 0.2 mmol / L DPPH ethanol solution, and mix thoroughly; Control group: Take 500 μL of sterile PBS buffer, add 500 μL of 0.2 mmol / L DPPH ethanol solution, and mix thoroughly; Blank group: Take 500 μL of bacterial suspension, add 500 μL of anhydrous ethanol solution, and mix thoroughly.

[0073] The experimental group, control group and blank group were placed on a shaker and oscillated for 30 min at 30°C in the dark. After the oscillation reaction, the reaction solution was centrifuged at 4000 rpm for 10 min.

[0074] Take 100 μL of supernatant and measure the absorbance at 517 nm with an enzyme marker. Repeat twice and calculate according to the DPPH free radical scavenging rate formula (5). The data are summarized in Table 7:

[0075] A: Fluorescence value of experimental group; B: Fluorescence value of blank group; C: Fluorescence value of the control group.

[0076] Table 7 Antioxidant capacity test results of Lactobacillus plantarum MPs-68

[0077] According to the data in Table 7, the scavenging rate of DPPH free radicals by Lactobacillus plantarum MPs-68 was (96.57±1.4)%, and its antioxidant capacity was very significant.

[0078] Example 5 Determination of the digestion resistance of Lactobacillus plantarum MPs-68 in simulated gastric and intestinal fluids in vitro Lactobacillus plantarum MPs-68 was activated for 2 generations and inoculated into MRS liquid culture medium at a 3% (v / v) inoculation amount. The culture was cultured at 37°C for 24 h, and the culture was centrifuged at 4°C and 8000 rpm for 15 min. The supernatant was discarded and the bacteria were collected.

[0079] Wash the cells twice with 0.85% sterile saline, and resuspend the cells with 0.85% sterile saline to adjust the concentration of the bacterial solution to 1×10 11 CFU / mL, and obtain the bacterial suspension of Lactobacillus plantarum MPs-68 for later use.

[0080] 5.1 Simulated gastric acid resistance test Preparation of simulated gastric fluid: Prepare pepsin (p7000) into a pepsin solution with a concentration of 2 g / L using 0.85% sterile saline, adjust the pH to 3.0 using 4 mol / L hydrochloric acid solution, and then filter and sterilize using a 0.22 μm microporous filter membrane for later use.

[0081] Pipette 1 mL of the bacterial suspension, add it to 9 mL of the simulated gastric fluid, mix thoroughly, take 1 mL of the mixture and dilute it 10 times to a certain gradient, oscillate evenly, take 1 mL of the diluted mixture, and determine the initial viable count by the pour plate method.

[0082] The remaining diluted mixture was incubated at 37°C for 2 h, and the number of viable bacteria was determined using the same method. The survival rate of strains in simulated gastric fluid was calculated according to formula (6), and the data are summarized in Table 8.

[0083]

[0084] N0: initial viable bacterial count after treatment with simulated gastric fluid, CFU / mL; N1: The number of viable bacteria after 2 h of incubation in simulated gastric fluid, CFU / mL.

[0085] 5.2 Simulated intestinal fluid bile salt tolerance test Preparation of simulated intestinal fluid: Use 0.85% sterile saline to prepare trypsin (USP (P7545)) into a trypsin solution with a concentration of 1 mg / mL, then add 0.2% ox bile salt, adjust the pH to 8.0 with 1 mol / L sodium hydroxide solution, and then filter and sterilize with a 0.22 μm microporous filter membrane for use.

[0086] 1 mL of the MPs-68 mixture incubated in simulated gastric fluid for 2 h was taken and added to 9 mL of the simulated intestinal fluid. The mixture was mixed thoroughly and incubated again at 37 °C for 1 h. 1 mL of the mixture was taken and diluted 10-fold to a certain gradient. The mixture was oscillated evenly. 1 mL of the diluted mixture was taken and the initial viable bacterial count was determined by the pour plate method. The survival rate of the strain in simulated intestinal fluid was calculated according to formula (7). The data are summarized in Table 8.

[0087]

[0088] N1: number of viable bacteria after 2 h of incubation in simulated gastric fluid, CFU / mL; N2: number of viable bacteria after 1 h of incubation in simulated intestinal fluid, CFU / mL.

[0089] Table 8 Results of in vitro test on the digestion resistance of Lactobacillus plantarum MPs-68 in simulated gastric and intestinal fluids

[0090] According to the data in Table 8, Lactobacillus plantarum MPs-68 grew very well in simulated gastric fluid, and the survival rate of the strain was 99.23% after 2 hours of incubation, indicating that after Lactobacillus plantarum MPs-68 entered the gastric fluid environment, the lower pH environment in the gastric fluid would only cause a small number of bacteria to die, and most of the bacteria could survive. It can be seen that Lactobacillus plantarum MPs-68 has a strong tolerance to acid and pepsin and can survive well in the simulated gastric fluid environment.

[0091] After incubation in the simulated gastric fluid environment for 2 hours, Lactobacillus plantarum MPs-68 entered the intestinal fluid environment. After incubation for 1 hour, the strain survival rate was 96.26%, indicating that after Lactobacillus plantarum MPs-68 entered the intestinal fluid environment, the high concentration of bile salts in the intestinal fluid would only kill a part of the bacteria, while a considerable number of bacteria could tolerate and survive. It can be seen that Lactobacillus plantarum MPs-68 can tolerate the high bile salt environment in the intestine and has the ability to tolerate trypsin, and can survive well in the simulated intestinal fluid environment.

[0092] In summary, the Lactobacillus plantarum MPs-68 provided by the present invention grows well after a series of digestions in simulated gastric juice and intestinal juice, and can survive well in the intestinal environment. The ability of the strain to enter the intestine and survive and colonize in the intestine is a prerequisite for its function. It can be seen that the Lactobacillus plantarum MPs-68 provided by the present invention has a high intestinal application value.

[0093] Example 6 Lactobacillus plantarum MPs-68 bacterial agent The present invention provides a Lactobacillus plantarum MPs-68 bacterial agent, which is prepared according to the following steps: Lactobacillus plantarum MPs-68 was activated and inoculated at a 3% (v / v) inoculum into MRS liquid culture medium sterilized at 121°C for 15 min. 12% skim milk, 2.0% glucose, 1.5% peptone, 0.6% yeast extract and the remainder water were added to the total mass of the culture medium to adjust the pH to 6.8.

[0094] The cells were cultured at 37°C for 48 h, centrifuged at 4°C and 6000 rpm for 20 min, the supernatant was discarded, and phosphate buffer (pH = 7.2) was added to wash 2 to 4 times to obtain bacterial sludge.

[0095] The bacterial mud and the protective agent are mixed and emulsified in a mass ratio of 1:1; wherein the protective agent includes 150 g / L crystalline trehalose, 40 mL / L glycerol, 30 g / L isomaltooligosaccharide, 25 g / L fructooligosaccharide and 10 g / L sodium L-glutamate.

[0096] The emulsion was pre-frozen at -40°C for 2 h and then freeze-dried. After freeze-drying, the bacterial survival rate of Lactobacillus plantarum MPs-68 was calculated according to the bacterial survival rate formula (8).

[0097]

[0098] N: number of viable bacteria after reconstitution of freeze-dried bacterial powder, CFU / mL; N0: number of viable bacteria in the emulsion before freeze-drying, CFU / mL.

[0099] It can be calculated that the survival rate of the Lactobacillus plantarum MPs-68 provided by the present invention after freeze-drying is 96.8%, and the activity retention degree of the strain is excellent.

[0100] Example 7 Lactobacillus plantarum MPs-68 probiotic product The present invention provides a probiotic product of Lactobacillus plantarum MPs-68, which is any one of a compressed candy, a solid beverage, a beverage, a granule, a capsule, a tablet, a pill and an oral liquid, and its components include Lactobacillus plantarum MPs-68 capable of absorbing microplastics and degrading uric acid and ingredients; Among them, the ingredients can be any one of prebiotics, fillers, acidulants, solvents, propellants, solubilizers, cosolvents, emulsifiers, colorants, adhesives, disintegrants, lubricants, wetting agents, osmotic pressure regulators, stabilizers, glidants, flavoring agents, preservatives, suspending agents, coating materials, fragrances, anti-adhesive agents, integrators, penetration enhancers, pH regulators, buffers, plasticizers, surfactants, defoamers, thickeners, inclusion agents, humectants, absorbents, diluents, flocculants, deflocculating agents and filter aids, or any combination of any two or more of them.

[0101] 7.1 Lactobacillus plantarum tablet candy In this embodiment, a Lactobacillus plantarum MPs-68 tablet candy is provided, which is prepared according to the following steps: Preparation of active bacterial powder: activating Lactobacillus plantarum MPs-68 capable of absorbing microplastics and degrading uric acid, culturing it in MRS liquid culture medium, collecting and washing the bacteria, adding auxiliary materials, and drying to obtain active bacterial powder; Take 5 parts of active bacteria powder, 60 parts of sorbitol, 12 parts of whole milk powder, 8 parts of fermented kiwi powder, 2 parts of isomaltooligosaccharide, 2 parts of magnesium stearate, 0.5 parts of vitamin C, 2 parts of galacto-oligosaccharide, 2 parts of fructo-oligosaccharide, and 2 parts of oligo-glucose by weight, mix well to obtain pre-made powder; The prefabricated powder is tableted and packaged using a candy tableting device to obtain a Lactobacillus plantarum MPs-68 tablet candy, wherein the number of viable bacteria of the Lactobacillus plantarum MPs-68 is not less than 1×10 11 CFU / g.

[0102] 7.2 Lactobacillus plantarum probiotic solid beverage In this embodiment, a probiotic solid beverage of Lactobacillus plantarum MPs-68 is also provided, which is prepared according to the following steps: Preparation of active bacterial powder: activating Lactobacillus plantarum MPs-68 capable of absorbing microplastics and degrading uric acid, culturing it in MRS liquid culture medium, collecting and washing the bacteria, adding auxiliary materials, and drying to obtain active bacterial powder; Take 20% active bacteria powder, 15% bonito peptide, 10% erythritol, 15% skim milk powder, 15% fermented kiwi powder, 10% maltodextrin, 5% fructooligosaccharide, 5% galacto-oligosaccharide, and 5% isomaltooligosaccharide by mass fraction, mix well, and package to obtain a Lactobacillus plantarum MPs-68 probiotic solid beverage; Wherein, the viable count of Lactobacillus plantarum MPs-68 is not less than 1×10 10 CFU / g.

[0103] It should be noted that the above-mentioned concept is to mix the active bacterial powder of Lactobacillus plantarum MPs-68 with the ability to adsorb microplastics and degrade uric acid or other forms of Lactobacillus plantarum MPs-68 with the ability to adsorb microplastics and degrade uric acid with other ingredients to prepare a probiotic product. The selection and ratio of the ingredients can be adaptively adjusted, including but not limited to maltodextrin, fructooligosaccharides, galacto-oligosaccharides, etc. in the embodiment scheme.

[0104] Example 8 Lactobacillus plantarum MPs-68 fermentation product The present invention provides a Lactobacillus plantarum MPs-68 fermentation product, which is prepared according to the following steps: Preparation of active bacterial powder: activating Lactobacillus plantarum MPs-68 capable of absorbing microplastics and degrading uric acid, culturing it in MRS liquid culture medium, collecting and washing the bacteria, adding auxiliary materials, and drying to obtain active bacterial powder; Weigh 2 kg of water, 0.5 kg of concentrated blueberry juice and 0.6 kg of concentrated pineapple juice, and mix them thoroughly to obtain a fermentation culture medium; Weigh 63 g of active bacterial powder, inoculate it into the fermentation culture solution, ferment at 32° C. for 48 h, and control the pH at the fermentation end point to be 3.62, thereby obtaining a fermentation product of Lactobacillus plantarum MPs-68; Wherein, the viable count of Lactobacillus plantarum MPs-68 is ≥ 1×10 11 CFU / g or 1×10 11 CFU / mL.

[0105] It should be noted that the above-mentioned concept is to add active bacterial powder of Lactobacillus plantarum MPs-68 that has the ability to adsorb microplastics and degrade uric acid or other forms of Lactobacillus plantarum MPs-68 that has the ability to adsorb microplastics and degrade uric acid to the fermentation system to obtain a fermented product. The selection and ratio of each component of the fermentation culture medium in the fermentation system can be adaptively adjusted, including but not limited to concentrated blueberry juice, concentrated pineapple juice, etc. in the embodiment scheme.

[0106] Example 9 Lactobacillus plantarum MPs-68 microecological preparation The present invention provides a microecological preparation, the components of which include active bacterial powder of Lactobacillus plantarum MPs-68 capable of absorbing microplastics and degrading uric acid and / or fermentation liquid of Lactobacillus plantarum MPs-68 capable of absorbing microplastics and degrading uric acid; The active bacterial powder of Lactobacillus plantarum MPs-68 capable of absorbing microplastics and degrading uric acid is vacuum freeze-dried bacterial powder and / or spray-dried bacterial powder, and the number of viable bacteria is ≥ 1×10 11 CFU / g; The Lactobacillus plantarum MPs-68 fermentation broth capable of absorbing microplastics and degrading uric acid includes any one of a fermentation supernatant, a cell-free extract, and bacterial cell metabolites, or a combination of any two or more thereof.

[0107] Example 10 Microplastic Adsorbent The present invention provides a microplastic adsorbent, whose components include Lactobacillus plantarum MPs-68 which has the function of adsorbing microplastics and degrading uric acid, or a composite bacteria including Lactobacillus plantarum MPs-68 which has the function of adsorbing microplastics and degrading uric acid and Lactobacillus curvatus MPA01.

[0108] 10.1 Lactobacillus plantarum MPs-68 microplastic adsorbent In this embodiment, a Lactobacillus plantarum MPs-68 microplastic adsorbent is provided, which is prepared according to the following steps: A single colony of Lactobacillus plantarum MPs-68 was selected and inoculated into MRS liquid culture medium and cultured at 37°C for 24 h to obtain an activated solution; The activated solution was inoculated into MRS liquid medium at a rate of 3% (v / v) and cultured at 37°C for 20 h to obtain the primary seed solution; The first-level seed solution was inoculated into MRS liquid medium at an inoculum volume of 3% (v / v), and cultured at 37°C for 20 h to obtain the second-level seed solution; The secondary seed liquid was inoculated into MRS liquid medium at an inoculum volume of 3% (v / v), and cultured at 37°C for 20 hours to obtain bacterial liquid; The bacterial solution was centrifuged at 6000 rpm for 10 min, and the precipitate was collected; The precipitate was washed twice with sterile PBS buffer (pH = 7.4), centrifuged again at 6000 rpm for 10 min, the supernatant was discarded, and the bacteria were collected; A protective agent solution containing 100 g / L skim milk powder, 30 g / L crystalline trehalose, 20 g / L oligofructose and 10 g / L sodium glutamate is emulsified and mixed with the Lactobacillus plantarum MPs-68 bacteria in a weight ratio of 1:1, and freeze-dried to obtain a Lactobacillus plantarum MPs-68 microplastic adsorbent; Among them, the number of viable Lactobacillus plantarum MPs-68 was ≥ 1×10 11 CFU / g.

[0109] 10.2 Composite microplastic adsorbent of Lactobacillus plantarum MPs-68 and Lactobacillus curvatum MPA01 In this embodiment, a composite microplastic adsorbent of Lactobacillus plantarum MPs-68 and Lactobacillus curvatum MPA01 is also provided, which is prepared according to the following steps: Single colonies of Lactobacillus plantarum MPs-68 and Lactobacillus curvatus MPA01 were selected and inoculated into MRS liquid culture medium, and cultured at 37°C for 24 h to obtain activation solution. The activated solution was inoculated into MRS liquid culture medium at an inoculum volume of 3% (v / v) and cultured at 37°C for 20 h to obtain the primary seed solution; The first-level seed solution was inoculated into MRS liquid medium at an inoculum volume of 3% (v / v) and cultured at 37°C for 20 h to obtain the second-level seed solution; The secondary seed solution was inoculated into the same MRS liquid medium at a 3% (v / v) inoculation rate and cultured at 37°C for 20 h to obtain a composite bacterial solution. The composite bacterial solution was centrifuged at 6000 rpm for 10 min to collect the precipitate; The precipitate was washed twice with sterile PBS buffer (pH = 7.4), centrifuged again at 6000 rpm for 10 min, the supernatant was discarded, and the composite bacterial cells were collected; A protective agent solution containing 100 g / L skim milk powder, 30 g / L crystalline trehalose, 20 g / L fructooligosaccharide and 10 g / L sodium glutamate was emulsified and mixed with the composite bacterial cells in a weight ratio of 1:1, and freeze-dried to obtain a composite microplastic adsorbent of Lactobacillus plantarum MPs-68 and Lactobacillus curvatus MPA01; Among them, the number of viable bacteria of the composite bacteria is ≥ 1×10 11 CFU / g.

[0110] It should be noted that in the present embodiment, the number of live bacteria of Lactobacillus plantarum MPs-68: the number of live bacteria of Lactobacillus curvatus MPA01 in the composite bacteria is 1:1. Those skilled in the art can adaptively adjust the ratio according to the functional effects of Lactobacillus plantarum MPs-68 and Lactobacillus curvatus MPA01 provided by the present invention to meet corresponding needs.

[0111] The Lactobacillus plantarum MPs-68 with the ability to adsorb microplastics and degrade uric acid provided by the present invention is separated and screened from sweet osmanthus fish roe samples aseptically collected by marine fishermen in Xiamen City, Fujian Province. It is a natural strain of marine origin, has high food safety, and has a significant adsorption effect on microplastics PS and PE, with adsorption rates of 62.14% and 48.28%, respectively. After being combined with Lactobacillus curvatus MPA01, the microplastic adsorption capacity can be further improved, and the adsorption rates of PS and PE reach 66.25% and 52.70%, respectively. In addition, it also has the ability to degrade uric acid, with a degradation rate of 74.59%, and the degradation effect is significant. The strain has a DPPH free radical scavenging rate of (96.57±1.4)%, has significant antioxidant ability, is resistant to gastric acid and bile salts, can effectively colonize in the intestine, and has significant commercial value.

[0112] The Lactobacillus plantarum MPs-68 with the ability to adsorb microplastics and degrade uric acid provided by the present invention can be used as a raw material component of probiotic products, functional foods, fermented products, etc., but its efficacy includes but is not limited to the adsorption of microplastics, lowering uric acid and anti-oxidation, and all the efficacy that can be beneficial to the human body is acceptable. Among them, the product form includes solid preparations such as powders and tablets, or liquid preparations suitable for oral administration by humans.

[0113] It should be noted that, although the term "functional food" is used more frequently in this article, the term "food" is broad, including human food and drink. In certain embodiments, the food product is suitable for and designed for human consumption. The use of these terms is only for the purpose of more conveniently describing and explaining the essence of the present invention, and does not exclude the possibility of using other terms; interpreting them as any additional limitation is contrary to the spirit of the present invention.

[0114] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein by equivalents. However, these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A Lactobacillus plantarum MPs-68 capable of absorbing microplastics and degrading uric acid, characterized in that: The deposit number is CGMCC No.31976.

2. A plant lactobacillus MPs-68 bacterial agent, characterized in that: Its components include the Lactobacillus plantarum MPs-68 with the ability to adsorb microplastics and degrade uric acid as described in claim 1.

3. The plant lactobacillus MPs-68 bacterial agent according to claim 2, characterized in that: The number of viable bacteria of Lactobacillus plantarum MPs-68 is ≥ 1×10 11 CFU / g or ≥1×10 11 CFU / mL.

4. A probiotic product of Lactobacillus plantarum MPs-68, characterized in that: Its components include the Lactobacillus plantarum MPs-68 with the ability to adsorb microplastics and degrade uric acid as described in claim 1.

5. A functional food of Lactobacillus plantarum MPs-68, characterized in that: Its components include the Lactobacillus plantarum MPs-68 with the ability to adsorb microplastics and degrade uric acid as described in claim 1.

6. The functional food of Lactobacillus plantarum MPs-68 according to claim 5, characterized in that: The functional food includes any one of microplastic adsorbing functional food, uric acid degrading functional food, antioxidant functional food, acid-resistant functional food and bile-resistant functional food, or a combination of any two or more thereof.

7. A fermentation product of Lactobacillus plantarum MPs-68, characterized in that: Its components include the Lactobacillus plantarum MPs-68 with the ability to adsorb microplastics and degrade uric acid as described in claim 1.

8. A microecological preparation, characterized in that: Its components include the Lactobacillus plantarum MPs-68 with the ability to adsorb microplastics and degrade uric acid as described in claim 1.

9. A microplastic adsorbent, characterized in that: Its components include Lactobacillus plantarum MPs-68 or a composite bacteria of Lactobacillus plantarum MPs-68 and Lactobacillus curvatum MPA01; Wherein, the Lactobacillus plantarum MPs-68 is the Lactobacillus plantarum MPs-68 capable of adsorbing microplastics and degrading uric acid as claimed in claim 1, and the Lactobacillus curvatum MPA01 is deposited with CGMCC No.31977; The number of viable bacteria of Lactobacillus plantarum MPs-68 is ≥ 1×10 9 CFU / g or ≥1×10 9 CFU / mL, the viable count of the composite bacteria is ≥1×10 9 CFU / g or ≥1×10 9 CFU / mL.

10. Use of the microplastic adsorbent according to claim 9 in the adsorption of polystyrene microplastics and / or polyethylene microplastics.

Images