Rocking dynamic culture box

A dynamic culture and box technology, applied in the field of biotechnology or reproductive medicine, can solve the problems of complex composition, incomparable embryo quality and implantation rate, and complex embryo development environment, and achieve the effect of improving pregnancy rate.

CN102807952AInactive Publication Date: 2012-12-05NANJING GENERAL HOSPITAL NANJING MILLITARY COMMAND P L A
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2012-12-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a rocking dynamic culture box. The rocking dynamic culture box comprises a box body, a sliding groove, a motor, a reciprocating motion mechanism and a motor speed adjusting device, wherein a tray is arranged in the box body; the sliding groove is formed below the tray; a sliding block matched with the sliding groove is arranged at the bottom of the tray and connected with the sliding groove in a sliding way; the sliding groove is supported by a bracket; the reciprocating motion mechanism comprises a crank and a connecting rod; one end of the connecting rod is connected with the sliding block; the other end of the connecting rod is connected with an output shaft of the motor through the crank; the motor drives the crank to rotate and pushes the sliding block to reciprocate in the sliding groove through the connecting rod; and the motor speed adjusting device is connected with the motor. According to the rocking dynamic culture box, the crank and connecting rod mechanism is driven by the motor to drive the tray to reciprocate with a low frequency in a small range, so that an oocyte or embryo for in-vitro culture is positioned in a dynamic culture environment, so as to simulate the rocking state of the oocyte or embryo in uterus. Therefore, the embryo development quality can be remarkably promoted and the later embryo implantation can be promoted, so as to increase the pregnancy rate.
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Description

technical field

[0001] The invention relates to the field of biotechnology or reproductive medicine, in particular to an incubator capable of dynamically cultivating oocytes or embryos. Background technique

[0002] In animal embryo engineering research or human assisted reproductive technology, in vitro maturation of oocytes, fertilization or in vitro culture of pre-implantation embryos is a routine operation in the laboratory, mainly carried out in an incubator.

[0003] To improve the in vitro development quality of gametes or embryos, laboratory research mainly focuses on the optimization of medium components, addition of cytokines, appropriate oxygen concentration, etc. With the in-depth research of the laboratory research group, the in vitro culture system of embryos or gametes is becoming more and more perfect. Various culture conditions (gas phase, temperature) try to completely simulate the internal environment of the female reproductive system (uterus or fallopian ...

Examples

Embodiment 1

[0027] figure 1 It is a rocking dynamic incubator, comprising a box body 1 with a box door 101, a tray 2, a chute 4, a motor 7, a reciprocating movement mechanism and a motor speed regulating device 8 in the box body.

[0028] The chute 4 is arranged below the tray 2, and the bottom of the tray is provided with a slide block 3 that is compatible with the chute 4. The tray and the slide block can be an integral piece or separate pieces fixed to each other. Slide block 3 is contained in the chute and can slide in the chute. Chute 4 is supported by the frame. Tray 2 can reciprocate back and forth above the chute.

[0029] The motor speed regulating device 8 adopts an electronic speed regulating device, and the electronic speed regulating device is connected with the motor 7 . The speed of the output shaft 11 of the motor can be adjusted by the electronic speed regulating device.

[0030] The reciprocating mechanism is a crank-link mechanism. One end of the connecting rod 5 is...

Embodiment 2

[0041] The surface of the tray 2 is provided with strip-shaped slots, and the bottom of the culture dish used is also provided with strip-shaped convex lines matching the strip-shaped grooves.

[0042] The motor speed regulating device 8 adopts a mechanical speed regulating device, and the output shaft of the motor is connected with the crank linkage through the mechanical speed regulating device. The rotational speed of the motor output shaft 11 can be adjusted through the mechanical speed regulating device.

[0043] Other structures of this embodiment are the same as those of Embodiment 1.