Sperm screening and in vitro fertilization bionic device based on micro fluidic technology

A microfluidic technology, in vitro fertilization technology, applied in the field of microfluidic devices, can solve problems such as the success rate of test-tube baby technology is difficult to exceed 50%, embryo failure or abortion, embryo damage, etc., to achieve convenient application and low production cost , The effect of reducing the miscarriage rate

Pending Publication Date: 2017-12-15
NANJING JINGJING BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nevertheless, many operations in the process of assisted reproductive technology may cause damage to the embryo, which will eventually lead to the embryo not developing or miscarriage, which is also the reason why the success rate of IVF technology is difficult to exceed 50%.

Method used

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  • Sperm screening and in vitro fertilization bionic device based on micro fluidic technology
  • Sperm screening and in vitro fertilization bionic device based on micro fluidic technology
  • Sperm screening and in vitro fertilization bionic device based on micro fluidic technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Such as Figure 1-2 The shown bionic device for sperm screening and in vitro fertilization based on microfluidic technology includes a bionic device body 100, which is composed of a glass slide layer, a silica gel pipeline layer (SU-8 silica gel layer) and polydimethylsiloxane Oxane (PDMS) layer, the middle part of the silica gel pipeline layer is provided with a central pool 1, and the outside of the central pool 1 is provided with an inlet pool 2, a first outlet pool 3 and a second outlet pool 4, and the central pool 1 and The inlet pool 2, the first outlet pool 3 and the second outlet pool 4 communicate through the first pipeline 5, the second pipeline 6 and the third pipeline 7 respectively, and on the first pipeline 5, the second pipeline 6 and the third pipeline 7 A first sample loading port 8, a second sample loading port 9 and a third sample loading port 10 are also provided respectively.

[0058] Wherein, the central pool 1 is added with sperm culture fluid; t...

Embodiment 2

[0063] In the bionic device for sperm screening and in vitro fertilization based on microfluidic technology in Example 1, the sources of liquid in each small pool are as follows:

[0064] Semen samples may be of human or other animal origin;

[0065] The cervical mucus is derived from a male spouse, a female animal or is artificial cervical mucus, and the artificial cervical mucus includes egg white; the sperm culture fluid includes commercial Earle's fluid and BWW fluid; and the oviduct fluid includes HTF fluid and MHTF fluid; The follicle culture fluid includes commercial KSOM culture fluid and CZB culture fluid; the cumulus complex is obtained from a male partner or a female animal, and is obtained from the ampulla of oviduct.

Embodiment 3

[0067] Artificial cervical mucus can be obtained by diluting egg white with normal saline 1:2-1:5.

[0068] The sperm culture solution can be prepared by itself and consists of the following components: 0.721g of NaCl, 0.247g of MgSO4·7H2O, 1g of fructose, 3.581g of Na2HPO4, 0.136g of KH2PO4, and 1000ml of purified water.

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Abstract

The invention provides a sperm screening and in vitro fertilization bionic device based on micro fluidic technology. The sperm screening and in vitro fertilization bionic device based on micro fluidic technology comprises a bionic device main body; the bionic device main body is composed of a glass sheet layer, a silica gel pipeline layer, and a polydimethylsiloxane layer; a center cell is arranged at the middle part of the silica gel pipeline layer; an inlet cell, a first outlet cell, and a second outlet cell are arranged outside the center cell; the center cell is communicated with the inlet cell, the first outlet cell, and the second outlet cell via a first pipeline, a second pipeline, and a third pipeline respectively; the first pipeline is provided with a first sample adding port; the second pipeline is provided with a second sample adding port; and the third pipeline is provided with a third sample adding port. The sperm screening and in vitro fertilization bionic device is used for stimulating complete natural impregnation process, unfavorable influences of operation on gametes and embryos are avoided, and polyspermy is avoided; high quality embryos at stimulated natural impregnation states can be obtained, in vitro fertilization success rate is increased greatly, the potential causes of sterility can be identified, and a direction is provided for clinical treatment.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a microfluidic device, in particular to a bionic device for sperm screening and in vitro fertilization based on microfluidic technology and a preparation method for the bionic device for sperm screening and in vitro fertilization based on microfluidic technology and working methods. Background technique [0002] The worldwide incidence of infertility is about 15% to 20%, and there is an increasing trend year by year. According to the prediction of WHO, in the near future, reproductive health problems will be the third major disease threatening human health after cardiovascular and cerebrovascular diseases and tumors. The increase in the incidence of infertility and the liberalization of the national "two-child" policy have greatly promoted the development of assisted reproductive technology. The first generation of test-tube baby technology - in vitro fertilization-embryo transfer te...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00
CPCC12M21/06C12M23/16
Inventor 陆金春
Owner NANJING JINGJING BIOTECHNOLOGY CO LTD
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