In vitro maturation culture method of denuded oocytes of mice

Abstract

The invention discloses an in vitro maturation culture method of denuded oocytes of mice. The method comprises the following steps: (1) performing in vitro maturation culture on the denuded oocytes; (2) performing parthenogenetic activation on matured denuded oocytes; (3) performing in vitro fertilization on the matured denuded oocytes. According to the method, an environment for artificially simulating natural maturation of oocytes can be created, so that the denuded oocytes can efficiently maturate and grow in vitro and can be subjected to in vitro fertilization and embryonic development as same as normally growing oocytes after in vitro maturation culture. The practice proves that the in vitro maturation culture efficiency of the denuded oocytes of the system is high, the system is mature and stable, and the embryonic development quality of the denuded oocytes is high after in vitro natural fertilization, so that the application and popularization of the denuded oocytes in reproductive health of human beings and genetic breeding of modern livestock can be greatly facilitated. In addition, the operation of obtaining blastulae from the denuded oocytes is simplified and the denuded oocytes serving as ovum sources can be conveniently put into actual production.

Description

Technical field [0001] The invention relates to an in vitro maturation culture method of mouse naked eggs. Background technique [0002] In normal reproductive development, a large number of cumulus cells are wrapped around the oocyte. During natural ovulation, the oocytes and the surrounding cumulus cells are discharged into the fallopian tube to wait for the combination with sperm. In embryo engineering operations or assisted reproductive technology applications, a large number of cumulus cells are wrapped around the oocytes with high maturation efficiency, and the mature oocytes have the function of naturally combining with sperm to form an embryo. However, in the oocytes directly extracted from the ovary in clinical applications, more than half of the oocytes are not completely surrounded by cumulus cells. This type of oocytes has low maturation efficiency, and even if they can mature, they will not have it after maturation. The ability to combine with sperm is often directl...

AI Technical Summary

Problems solved by technology

Previously, in the in vitro maturation of naked eggs in the world, the conventional culture system can lead to the maturation and development of oocytes, but mature oocytes do not have the ability to fertilize with sperm, and cannot further develop in vitro to achieve uterine implantation blastocyst stage