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Umbilical cord mesenchymal stem cell technology for reproductive health

A stem cell and stem cell technology, applied in animal cells, vertebrate cells, bone/connective tissue cells, etc., can solve the problems of tumorigenicity, application limitation of embryonic stem cells, immune rejection, etc. Toxic and adverse effects

Inactive Publication Date: 2013-08-14
孙勇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Embryonic stem cells are totipotent and can differentiate into cells derived from various germ layers, but there are problems such as immune rejection, tumorigenicity and ethics, so the application of embryonic stem cells is limited

Method used

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Examples

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specific Embodiment approach

[0012] The present embodiment will specifically illustrate the umbilical cord mesenchymal stem cell technology for reproductive health care of the present invention. The umbilical cord is the tissue that connects the placenta and the fetus. It is cut off when the fetus is born. It contains a large number of mesenchymal stem cells and has strong proliferative capacity. It does not need to be matched for transplantation. The operating sequence of the present invention is as follows:

[0013] (1) Stem cell collection and processing, signing the maternal umbilical cord and umbilical cord blood donation consent form, obtaining maternal umbilical cord blood and umbilical cord under sterile conditions in the delivery room, and separating the umbilical cord blood in a 100-level laminar flow hood in the GMP workshop within 36 hours: retain appropriate The samples were subjected to quality inspection (HBsAg, HCV-Ab, TP-Ab, HIV1 / 2-Ab, CMV-IgM), centrifuged at 700 rpm for 10 minutes to re...

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Abstract

The invention provides a technology of isolating and culturing umbilical cord mesenchymal stem cells from umbilical cords of volunteers for reproductive health, relates to the fields of basic medicine and reproductive health medical science. Stem cells are isolated and purified from umbilical cord tissues of a volunteer, and cultured for multiplication in a particular culture system. When the total stem cells reach a certain number, the stem cells are used for maintenance of the reproductive system through intravenous infusion. The mesenchymal stem cells can be used for maintenance of reproductive system and treatment of dysfunction, and have the advantages that the source is rich, the cells are stored in kind and can be recovered or cultured and amplified when necessary, the in vitro amplification ability is high, tissue typing is not needed for transplantation, and the graft-versus-host disease (GVHD) and cytomegalovirus (CMV) infection do not occur. The umbilical cord mesenchymal stem cell technology for health care or treatment has no toxic side effects and adverse reactions.

Description

technical field [0001] The invention utilizes umbilical cord tissue to culture and expand stem cells that can be used for reproductive health care, and relates to the fields of basic medicine and reproductive health care science. Background technique [0002] In recent years, the importance of reproductive health is receiving increasing attention, as reproductive system activities not only affect the current society, but also directly affect the future of human society. Therefore, the health of reproductive system is no longer simply a subject in the field of medicine, but an eternal theme of the survival, development and progress of human society. For a long time, people often attribute the health of the reproductive system to gynecology, but in fact, the health of the reproductive system is not only for women, the health of the male reproductive system is also very important. The health of the reproductive system refers not only to the absence of abnormalities and disease...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A61K35/44A61P15/00A61K35/51
Inventor 孙勇
Owner 孙勇
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