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Sphingomonas capable of degrading triphenyl phosphate and domestication method and application thereof

A technology of sphingomonas and triphenyl phosphate, which is applied in the field of sphingomonas and its domestication, can solve the problems of low efficiency and achieve low cost and good degradation effect

Active Publication Date: 2019-09-20
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the microbial degradation of OPFRs is often not efficient in practical applications, so the screening and application of highly efficient OPFRs degrading bacteria has certain research significance

Method used

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  • Sphingomonas capable of degrading triphenyl phosphate and domestication method and application thereof
  • Sphingomonas capable of degrading triphenyl phosphate and domestication method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 1. Domestication and screening of degrading strains

[0049] (1) Add triphenyl phosphate-contaminated soil (taken from electronic waste-contaminated soil in Guiyu Town, Guangdong, where the content of triphenyl phosphate is 4260-1710000ng / g) into 20mL inorganic salt medium (sterilized) , cultivated in a constant temperature shaker at a temperature of 30° C. and a rotation speed of 160 rpm for 4 hours, then took it out, stood it for stratification, and obtained a supernatant. Each liter of inorganic salt medium includes: 1g / L NH 4 NO 3 , 1.5g / L KH 2 PO 4 , 3g / LK 2 HPO 4 , 2mL / L trace element solution, 1000mL distilled water, pH 7.0. The trace element solution is a solution obtained by adding trace element compounds into water and mixing them uniformly, wherein in the trace element solution, the concentration of each substance is: 4g / L MgSO 4 , 4g / LZnSO 4 , 1g / L CuSO 4 , 1g / L MnSO 4 , 1g / LFeSO 4 ·7H 2 O, 1g / LCaCl 2 ; the same below.

[0050] (2) Take 1 mL of...

Embodiment 2

[0059] Example 2 Degradation Analysis of Sphingomonas to Triphenyl Phosphate (Effect Verification)

[0060] (1) Inoculate Sphingomonas sp.GY-1 into a 500mL Erlenmeyer flask containing 200mL of sterilized enrichment medium, and place it in a constant temperature shaker at a temperature of 30°C and a rotational speed of 160rpm, cultivated for 24 hours, to obtain enriched cultured bacterial solution; each liter of enriched medium includes: 3g / L beef extract, 10g / L peptone, 5g / L NaCl, 1000mL distilled water, pH 7.0.

[0061] (2) Put the enriched cultured bacterial solution (20mL) in a 50mL sterilized centrifuge tube, centrifuge at 6000g for 5min, collect the bacteria, wash repeatedly with sterile normal saline and centrifuge for 3 times, and finally add normal saline to prepare into a certain concentration (OD 600 =0.6) of the bacterial suspension, 1mL of the configured bacterial suspension was added to the sterilized degradation medium (triphenyl phosphate degradation medium) co...

Embodiment 3

[0081] Example 3 is the same as Example 2, except that during the enrichment culture process, the pH of the enrichment medium is 6.5, and the culture conditions are as follows: the temperature is 20° C., the rotation speed of the shaker is 120 rpm, and the culture time is 16 hours. Finally, the degradation rate of triphenyl phosphate by Sphingomonas was determined to be 92.7%.

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Abstract

The invention discloses a sphingomonas capable of degrading triphenyl phosphate and a domestication method and an application thereof. The Sphingomonas is preserved in the Guangdong Provincial Collection of Microorganisms and Cultures on April 19, 2019, and a preservation number is GDMCC No: 60633. The domestication method of the strain comprises the following steps: using a gradient concentration method to perform domestication of the triphenyl phosphate degrading bacteria, and separating and purifying the material to obtain the Sphingomonas sp. GY-1. The strain has the good degradation effect on triphenyl phosphate, and the use of the bacteria to treat polluted water bodies is low in cost. The Sphingomonas sp.GY-1provided by the present invention has the degradation rate on triphenyl phosphate to 98.77% after 5d, and the practical application value is high, which can provide a reference for solving the pollution control problem of an organic phosphorus flame retardant in water.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental organic pollutants, and in particular relates to a sphingomonas capable of degrading triphenyl phosphate and its domestication method and application. Background technique [0002] Organophosphate flame retardants (organophosphorus flame retardants, OPFRs) are a new class of flame retardants, which have become substitutes for traditional flame retardants such as polybrominated diphenyl ethers and polychlorinated biphenyls due to their low corrosion, easy metabolism and high flame retardant efficiency. Taste. Today, the global flame retardant market demand is increasing at an annual growth rate of 5%, and China and other developing countries have a growing demand for OPFRs. [0003] Triphenyl phosphate (TPHP) is a representative class of flame retardants for OPFRs and is widely used in unsaturated polyester resins, polyvinyl chloride, thermoplastics, casting resins an...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/36C02F3/34C02F101/30C12R1/01
CPCC12N1/20C12N1/36C02F3/34C02F2101/30C12R2001/01C12N1/205
Inventor 尹华杨苑钰金利珠
Owner SOUTH CHINA UNIV OF TECH
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