Novel strain sphingosine gingivalis ZUTE03 for producing coenzyme Q10 and uses thereof

A technology of ZUTE03 and Sphingomonas, applied in bacteria, fermentation and other directions, can solve the problems of large loss of extraction solvent, cumbersome steps, time-consuming and laborious, and achieve the effects of saving extraction time, improving efficiency and reducing consumption.

Inactive Publication Date: 2008-05-14
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

The method of extracting coenzyme Q10 from fermented bacteria is mostly an extraction method after saponification of alcohol and alkali, the steps are cumbersome, time-consuming and laborious, and the loss of extraction solvent is large

Method used

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  • Novel strain sphingosine gingivalis ZUTE03 for producing coenzyme Q10 and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Isolation, identification and fermentation of coenzyme Q produced by Sphingomonas sp. ZUTE03 10 characteristic

[0022] 1. Isolation of strains:

[0023] (1) Collect samples

[0024] After collecting soil samples from the Jiubao section of Hangzhou along the Qiantang River, take 10 g and add 90 mL of sterile water to make a soil suspension.

[0025] (2) Strain isolation

[0026] Take 1 mL of bacterial suspension and dilute 10 5 times, streaked on the selective plate, the composition of the selective medium (g / L) is: isoprene 0.5ml, (NH 4 ) 2 SO 4 1.0g, KH 2 PO 4 4.5g, Na 2 HPO 4 12H 2 O 21.6g, agar 15-20g, water 1000ml, pH7.0.

[0027] According to the conventional method for the isolation of pure microorganisms, the above isolation culture was placed at 28° C. for 2 to 3 days. Pick multiple single colonies, inoculate them on the slant medium, and save them by number. Fermentability test was carried out in the liquid culture medium again, and ...

Embodiment 2

[0057] Example 2: Extraction of coenzyme Q from sphingomonas (Spingomonas sp.) ZUTE03 fermentation cells 10 Process optimization and simplification

[0058] Sphingomonas sp. (Spingomonas sp.) ZUTE03 was inoculated in optimized post-fermentation medium (glucose 15g, (NH 4 ) 2 SO 4 10g, KH 2 PO 4 0.5g, Na 2 HPO 4 1.5g, MgSO 4 0.5g, water 1000ml pH7.0), culture temperature 25℃, 200r / min shaker culture time 36h, take the fermentation cells, collect the fermentation liquid, centrifuge at 15000r / min for 15min, pour off the supernatant, take the cells, and use Fully washed with distilled water, and centrifuged to obtain the fermented cells.

[0059] Transfer the bacteria into a 150ml round-bottomed flask, and use the traditional saponification extraction method (specific method: transfer 2g of wet bacteria into the container, add 0.7g of pyrogallic acid, 2.5g of KOH, 19ml of methanol, 7ml of distilled water, and mix well. Reflux in a 90°C water bath for 30 minutes, quickl...

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Abstract

The invention provides a new strain Sphingomonas sp.ZUTE03 that can produce coenzyme Q10 and is preserved in China Center for Type Culture Collection (CCTCC) on June 18, 2007, and the preservation number is CCTCC No. M207084. When the leavening of the invention is used for preparing the coenzyme Q10, a new technology of synchronization saponification extraction is adopted, which can save manpower, reagent and time with convenience and can improve the efficiency of the extraction technique for the coenzyme Q10 under the condition of nearly no damage of products.

Description

(1) Technical field [0001] The invention relates to a strain producing coenzyme Q 10 The new strain—Sphingomonas ZUTE03 and its application belong to the field of microbial pharmacy. (2) Background technology [0002] coenzyme Q 10 (CoenzymeQ 10 , CoQ 10 ) is widely present in animals, plants and microorganisms, and is an important hydrogen transporter in the respiratory chain of biological cells, as well as a good biochemical drug. It has the functions of anti-oxidation, eliminating free radicals, and improving the body's immunity. It has been widely used in the treatment of various heart diseases, diabetes, cancer, acute and chronic hepatitis, Parkinson's disease and other diseases. Preparation of Coenzyme Q 10 The methods mainly include animal cell extraction method, tobacco leaf solanesol semi-synthesis method, complete synthesis method, microbial fermentation method or plant cell culture method. Compared with other methods, the microbial fermentation method has th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/26
Inventor 钟卫鸿柳华贵金凌邱乐泉吴石金陈建孟孙柯丹方建军
Owner ZHEJIANG UNIV OF TECH
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