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Dendrobium candidum endophyte with growth promotion function and use thereof

A technology of Dendrobium officinale and uses is applied in the field of endophytic bacteria of natural medicinal plants, which can solve the problems of less research on endophytic bacteria and no research report on tissue culture of Dendrobium officinale, and achieve the effect of shortening production time.

Inactive Publication Date: 2010-12-22
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] At present, there are many international studies on the endophytic fungi of Orchidaceae plants, but there are few studies on endophytic bacteria, and there is no research report on the use of endophytic bacteria in Dendrobium candidum to promote the growth of Dendrobium candidum tissue culture.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, the isolation culture method of Sphingomonas CCTCC NO: M 2010041, carries out the following steps successively:

[0030] 1. Collect the root samples of wild healthy Dendrobium officinale plants in Tonglu, Zhejiang, wash them thoroughly with tap water, and treat them with 25KHz ultrasonic waves for 5 minutes;

[0031] 2. Under aseptic conditions, wash the test material obtained in step 1 twice with sterile water, and then use 75% alcohol and saturated bleach solution to disinfect the surface of the test material successively, so as to kill the test material. microorganisms on the surface of materials;

[0032] 3. In a sterile environment, rinse the test material obtained in step 2 with sterile water for 3 times, take 100 μl of the sterile water rinse produced in the last rinse, and inoculate it in the basal NA medium, at 30°C After culturing for 24 hours, observe whether there are colonies. Based on this, verify whether this disinfection method completely...

Embodiment 2

[0040] Embodiment 2, the cultivation seed production method of Sphingomonas CCTCC NO:M 2010041, carries out the following steps successively:

[0041] 1. Transfer the Sphingomonas CCTCC NO: M 2010041 to the slant of the basic NA medium, and cultivate it at 30°C for 2 days; get the slant seeds;

[0042] The slant formula of the basic NA medium is: peptone 10g, beef extract 3g, NaCl 5g, agar 18g and H 2 O1000ml, pH=7.4~7.6.

[0043] 2. Use an inoculation loop to pick 0.5g of the slant strain obtained in step 1, inoculate it into a 250ml Erlenmeyer flask containing 100ml of LB liquid medium, and culture it at 28°C for 30h at a speed of 200r / m to obtain Sphingomonas CCTCC NO: M2010041 fermentation stock solution;

[0044] The formula of LB liquid medium is: peptone 10g, yeast extract 10g, NaCl 5g and H 2 O1000ml, pH=7.4~7.6.

Embodiment 3

[0045] Embodiment 3, sphingomonas CCTCC NO: M 2010041 promotes the growth of Dendrobium candidum tissue culture seedling growth experiment:

[0046] The following experiments all use the tissue-cultured seedlings of Dendrobium candidum that have been cultivated in the improved tissue culture medium of Dendrobium candidum as the experimental objects.

[0047] The formula of the improved Dendrobium candidum tissue culture medium is: adopt 1 / 2MS as basic medium, add banana juice 10% / L, agar 7g / L, sucrose 20g / L, adjust pH value to 5.8 with 1mol / L NaOH . That is: add 0.1L banana juice, 7g agar and 20g sucrose to every L of 1 / 2MS, and adjust the pH value to 5.8.

[0048] Experiment 1. Sphingomonas CCTCC NO: M 2010041 fermentation stock solution on growth promotion of Dendrobium officinale tissue culture seedlings 1:

[0049] Utilize the Sphingomonas CCTCC NO:M 2010041 fermented stock solution obtained in the above-mentioned Example 2, dilute the fermented stock solution with steri...

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PUM

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Abstract

The invention discloses a sphingomonas sp. strain with preservation name of Sphingomonas (SH1), preserved in China Centre for Type Culture Collection (CCTCC) in Wuhan University, China, wherein the preservation date thereof is February 4, 2010 with preservation number thereof being CCTCC No: M 2010041. The invention also discloses a use of the sphingomonas sp. strain applied to promoting growth of dendrobium candidum, comprising the following specific uses: inoculating raw fermenting liquid or fermented liquid of the sphingomonas sp. strain CCTCC No: M 2010041 into a root of a dendrobium candidum seedling so as to promote the growth of the dendrobium candidum seedling.

Description

technical field [0001] The invention relates to a natural medicinal plant endophytic bacterium-Dendrobium officinale endophytic bacterium and its application. Background technique [0002] Dendrobium officinale is the second largest genus in the Orchidaceae (Orchidaceae) plants, Dendrobium SW. Nourishing yin and clearing heat, promoting body fluid and benefiting stomach, moistening lung and relieving cough, improving eyesight and strengthening body, analgesic and anti-inflammation; modern pharmacological research shows that dendrobium also has anti-tumor, anti-aging, enhancing body immunity, dilating blood vessels and anti-platelet aggregation and other effects. Due to its great medicinal value, it has become the lifeblood of raw materials for pharmaceutical factories and health care product processing enterprises that use Dendrobium. However, the growth conditions of Dendrobium officinale are very harsh, so the natural output is extremely rare, and due to long-term over-ha...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P21/00C12R1/01
Inventor 胡秀芳赵凯鹏俞婕张珊珊董飞陈集双
Owner ZHEJIANG SCI-TECH UNIV
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