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A kind of production method of Euonymus peach leaf regeneration plant

A technology of euonymus peach leaf and regenerated plants, which is applied in the field of plant tissue culture, can solve the problems of strong time requirements for sowing and propagation seeds collection, high management requirements, complicated pretreatment, etc., and achieves improved reproduction coefficient and survival rate, simple operation method, The effect of short cultivation period

Active Publication Date: 2018-05-18
金树林生态科技(南京)有限公司
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Problems solved by technology

[0003] The propagation of Euonymus peach leaf generally adopts methods such as sowing, cutting, and division propagation. However, due to the limitation of the number of seed gardens and the output, there are very few high-quality seedlings obtained, and the propagation speed is relatively slow. The time required for sowing and propagating seeds is very strong. The post-pretreatment is complicated and the post-management requirements are high. It is difficult to use the method of seed propagation to cultivate seedlings on a large scale in production. How to use the method of plant tissue culture to select high-quality seedlings has become the focus of attention in the process of Euonymus peach leaf propagation

Method used

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  • A kind of production method of Euonymus peach leaf regeneration plant
  • A kind of production method of Euonymus peach leaf regeneration plant
  • A kind of production method of Euonymus peach leaf regeneration plant

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Experimental program
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Effect test

Embodiment Construction

[0020] 1. Selection of Propagation Material

[0021] The test materials were taken from the biennial leaves of Euonymus persicae at the experimental station of Jinling Institute of Science and Technology.

[0022] 2. Disinfection of Propagation Material

[0023] Rinse the material with running water for 1 hour to remove surface impurities. On the aseptic operation bench, first rinse with sterile water for 3 times, then disinfect with 75% absolute ethanol for 30 seconds, then rinse with sterile water for 3 times, and then use 0.1% Mercury chloride disinfection treatment for 5-7 minutes, and finally rinsed with sterilized sterile water for 4 times; see attached figure 1 .

[0024] 3. Callus Induction

[0025] On the ultra-clean workbench, cut the sterilized leaves into 6mm squares and inoculate them on the callus induction medium, with the back of the leaves closely attached to the medium, inoculate 3-4 pieces per bottle, and induce light conditions of 14h / d , light intensit...

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Abstract

The invention belongs to the technical field of plant tissue culture, and particularly relates to a production method of a Euonymus bungeanus leaf regenerated plant. The production method comprises the following steps: selection and disinfection of explant, induction of callus, induction of adventitious bud and induction of root. The method has the advantages of short culture period, fewer variations, artificially controllable culture conditions and simple operating process, and lays the foundation for seed breeding, genetic transformation, breed improvement and other researches of Euonymus bungeanus as an excellent scientific research material, thereby providing guarantees for large-area production of Euonymus bungeanus tissue culture seedlings.

Description

a technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for producing regenerated plants of Euonymus persicae leaves. Two technical background [0002] Because of its good wood material, high oil content in seeds, high ornamental value, and large area of ​​plantation, Euonymus has become one of the most potential garden tree species in the 21st century to improve the living environment of human beings and meet the needs of human life. [0003] The propagation of Euonymus peach leaf generally adopts methods such as sowing, cutting, and division propagation. However, due to the limitation of the number of seed gardens and the output, there are very few high-quality seedlings obtained, and the propagation speed is relatively slow. The time required for sowing and propagating seeds is very strong. The post-pretreatment is complicated and the post-management requirements are high. It is difficult to use ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 马艳程馨慧尚静翟亚娇肖木株
Owner 金树林生态科技(南京)有限公司
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