Intelligent constant-temperature culture system and method for algae

Abstract

The invention discloses an intelligent constant-temperature culture system for algae. The system comprises an intelligent control unit, a constant-temperature circulating water unit and an algae culture unit, the intelligent control unit is used for intelligently regulating and controlling the temperature of the constant-temperature circulating water unit, so that the temperature of water entering the algae culture unit is constant, and meanwhile, various water quality parameter probes are arranged in the algae culture unit and are used for transmitting real-time detection parameters in the algae culture stage back to the intelligent control unit for data storage and real-time display. The device is reasonable in structural design and excellent in function, and does not cause large cold and hot impact on a culture sample in a constant temperature process to affect the growth of the culture sample. In addition, parameters such as air supply time and flow can be intelligently adjusted and set in combination with the real-time growth parameter state of the algae, it is ensured that the optimal air supply effect is achieved, and the cellular morphological structure is not affected or growth is inhibited while the algae culture growth rate is increased.

Description

Technical Field

[0001] The present invention relates to the technical field of intelligent constant temperature cultivation of algae, and in particular to an intelligent constant temperature cultivation system and method for algae. Background Art

[0002] The algae culture unit is mainly used for the cultivation of algae plants, providing controllable growth conditions such as temperature, humidity, and light for algae plants from growth to maturity. It can also be used in research on life sciences, cell microorganisms, molecular genetics, etc. It is an ideal auxiliary equipment commonly used in scientific research and production in many fields such as bioengineering, medicine, agronomy, aquaculture, and animal husbandry. Summary of the invention

[0003] The present invention provides an intelligent constant temperature cultivation system for algae, comprising an intelligent control unit, a constant temperature circulating water unit, and an algae cultivation unit; the intelligent control unit is used to intelligently control the temperature of the constant temperature circulating water unit so that the temperature of water entering the algae cultivation unit is constant, and at the same time, the algae cultivation unit is built with various water quality parameter probes, which are used to transmit real-time detection parameters of the algae cultivation stage back to the intelligent control unit for data storage and real-time display.

[0004] Furthermore, the intelligent control unit includes a touch display, an intelligent air supply device, and an electrical compartment; The touch display controls and adjusts the temperature of the constant temperature circulating water unit through the PLC master control unit in the electrical compartment to ensure that the water temperature entering the algae cultivation unit through the circulating water inlet is constant; at the same time, the touch display receives the pH, conductivity, dissolved oxygen, temperature, and light parameters monitored in real time by various probes in the algae cultivation unit, and the collector in the electrical compartment collects and transmits the monitored parameters in real time to the touch display to generate a parameter change curve; the touch display controls and operates the PLC master control unit in the electrical compartment to adjust the intelligent gas replenishment device to replenish the carbon dioxide mixed gas to the algae cultivation unit; The intelligent air replenishment device includes a gas mixer, an air pump, a carbon dioxide cylinder, and a 6-way gas distribution valve for mixed gas; the gas mixer is connected to the air pump and the carbon dioxide cylinder, and the intelligent control unit sets the ventilation flow and ventilation time to control the operation of the air pump. The air is compressed by the air pump, mixed with carbon dioxide and introduced into the gas mixer; the mixed gas is transported to the 6-way gas distribution valve for mixed gas through the mixed gas outlet interface under the action of the air pump pressure, and connected to different algae culture vessels as needed.

[0005] Furthermore, the constant temperature circulating water unit comprises a constant temperature unit, a constant temperature water tank, and a circulating water device; The constant temperature unit compresses the refrigerant into high-temperature and high-pressure gas by the compressor according to the temperature set by the intelligent control unit, enters the condenser to condense into liquid, passes through the drying filter to remove impurities, and then flows into the evaporator. The liquid refrigerant quickly vaporizes and absorbs heat, and the temperature of the constant temperature water tank is adjusted to ensure constant; The constant temperature water tank is equipped with a heating rod and a water level sensor. When the temperature is lower than the set value, the heating rod operates. When the temperature is higher than the set value, the heating rod is in standby mode. The water level sensor is used to accurately measure the water level and provide real-time feedback to the touch display. When the water level is lower than the minimum value, the intelligent control unit reminds manual water replenishment. The circulating water device is composed of a water pump and a circulating pipe body. The water pump transports circulating water with constant temperature to the algae cultivation unit through the circulating pipe body to keep the temperature in the algae constant temperature pool stable.

[0006] Furthermore, the algae cultivation unit includes an algae constant temperature pool, an algae incubator, a combined LED multi-color light panel, and a monitoring probe; The algae constant temperature pool is a transparent water tank made of acrylic sheet, with a built-in algae incubator, and a circulating water device to keep the temperature constant; The algae culture device comprises a plurality of detachable algae culture test tubes, and a built-in ventilation pipe for connecting a 6-way gas distribution valve to realize intelligent gas replenishment; The monitoring probes include a temperature probe, a pH probe, a conductivity probe, a dissolved oxygen probe and / or a light intensity probe, which transmit the monitoring parameters to the touch display in real time; The combined LED multi-color light panel is placed inside the algae constant temperature pool. The dimmer controls the LED light source intensity and adjusts the light source type. The lamp beads distributed in the LED matrix provide light of different colors for algae cultivation.

[0007] The present invention also discloses an operation method of the above algae intelligent constant temperature culture system, which is operated according to the following steps: 1. Turn on the power switch to put the device into operation / standby state; 2. Inject coolant into the constant temperature water tank of the equipment to the required water level; 3. Inject culture solution into the algae culture vessel until the amount required for the experiment; 4. The inlet / outlet of the thermostatic unit is connected to the inlet / outlet of the thermostatic pool; 5. Set the constant temperature water tank temperature on the touch display; 6. Set the required light intensity and type through the dimmer; 7. Turn on the circulating water system; 8. Start the equipment and observe the operating status; 9. The intelligent air supply device is turned on, the interval time is set, and the ratio of air and carbon dioxide is adjusted; 10. The pH probe, conductivity probe, dissolved oxygen probe, temperature probe and / or light probe are inserted into the algae culture vessel to monitor and collect values.

[0008] The beneficial effects of the present invention are: 1. Constant temperature: This equipment uses an external cold source to cool the algae culture device through a constant temperature water tank to maintain a constant temperature. First, the equipment uses a constant temperature water tank to reduce the coolant to the required temperature, and then circulates the cooling water to the algae constant temperature pool through a circulating water pump. The advantage of this approach is that it will not cause a large cold and heat shock to the culture sample and affect its growth.

[0009] 2. Intelligent gas replenishment: When the intelligent control unit determines that gas replenishment is needed, the gas replenishment device automatically starts. The air pump works to increase the pressure and flow of the mixed gas, and the carbon dioxide cylinder releases the corresponding gas. After being mixed by the gas mixer, the mixed gas is accurately passed into each algae culture device through the 6-way gas valve to improve the growth efficiency of algae. The advantage is that different algae have different sensitivities to carbon dioxide concentrations. The system can combine the real-time growth parameter status of algae to intelligently adjust and set parameters such as the time and flow of gas replenishment to ensure that the most suitable gas replenishment effect is achieved, which promotes the growth rate of algae culture without affecting the cell morphology or inhibiting its growth. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 This is the hardware composition diagram of the algae intelligent constant temperature cultivation system; Figure 2 This is the electrical control diagram of the algae intelligent constant temperature cultivation system; Figure 3 This is a diagram of the internal device of the algae cultivation unit; Figure 4 The growth status diagrams of Microcystis aeruginosa cultured using the system of the present invention on the 1st (upper left), 5th (upper right), 10th (lower left), and 15th (lower right) days; Figure 5 The figure is a comparison diagram of the changes in the density of algae cells cultured using the system of the present invention and a traditional culture box. DETAILED DESCRIPTION

[0011] The present invention will be further described below in conjunction with the embodiments.

[0012] Example An intelligent constant temperature algae cultivation system and method, comprising an intelligent control unit, a constant temperature circulating water unit, and an algae cultivation unit. The temperature of the constant temperature circulating water unit is intelligently controlled by the intelligent control unit, so that the water temperature entering the algae cultivation unit is constant. At the same time, the algae cultivation unit is built with various water quality parameter probes, and the real-time detection parameters of the algae cultivation stage are transmitted back to the intelligent control unit for data storage and real-time display. The intelligent control unit includes a touch display, an intelligent gas replenishment device, and an electrical compartment. The touch display controls and adjusts the temperature of the constant temperature circulating water unit through the PLC general control unit in the electrical compartment to ensure that the water temperature entering the algae cultivation unit through the circulating water inlet is constant, thereby promoting the growth and cultivation of algae. At the same time, the touch display receives the pH, conductivity, dissolved oxygen, temperature, light and other parameters monitored in real time by various probes in the algae cultivation unit, and the collector in the electrical compartment collects and transmits the monitoring parameters in real time, and transmits them to the touch display to generate a parameter change curve. The touch display controls the PLC general control unit in the electrical compartment to adjust the intelligent gas replenishment device to supplement the carbon dioxide mixed gas to the algae cultivation unit. The intelligent air replenishment device includes a gas mixer, an air pump, a carbon dioxide gas cylinder, and a 6-way gas distributor for mixed gas. The gas mixer is connected to the air pump and the carbon dioxide gas cylinder. The intelligent control unit sets the ventilation flow and ventilation time, controls the operation of the air pump, and the air is compressed by the air pump, mixed with carbon dioxide in a specific proportion and introduced into the gas mixer. Under the action of the air pump pressure, the mixed gas is transported to the 6-way gas distributor for mixed gas through the mixed gas outlet interface, and connected to different algae culture vessels as needed. The constant temperature circulating water unit is composed of a constant temperature unit, a constant temperature water tank, and a circulating water device. The constant temperature unit compresses the refrigerant into a high-temperature and high-pressure gas by a compressor according to the temperature set by the intelligent control unit, enters the condenser to condense into a liquid state, passes through a drying filter to remove impurities, and then flows into the evaporator. The liquid refrigerant quickly vaporizes and absorbs heat, and is adjusted to ensure that the temperature of the constant temperature water tank is constant. The constant temperature water tank is equipped with a heating rod and a water level sensor. When the temperature is lower than the set value, the heating rod operates. When the temperature is higher than the set value, the heating rod is in standby mode. The water level sensor can accurately measure the water level and feed it back to the touch display in real time. When the water level is lower than the minimum value, the intelligent control unit reminds manual water replenishment. The circulating water device is composed of a water pump and a circulating pipe body. The water pump transports the circulating water with constant temperature to the algae cultivation unit through the circulating pipe body to maintain the stability of the temperature in the algae constant temperature pool. The algae cultivation unit is composed of an algae constant temperature pool, an algae incubator, a combined LED multi-color light board, and various monitoring probes. The algae constant temperature pool is a transparent water tank made of acrylic sheet, with a built-in algae incubator, and the temperature is kept constant by the circulating water device. The algae incubator contains multiple algae culture tubes, which can be disassembled at will. The built-in ventilation pipe is used to connect the 6-way air distribution valve to realize intelligent air replenishment during the algae cultivation process.The various monitoring probes are placed in the algae culture device as needed, including temperature probe, pH probe, conductivity probe, dissolved oxygen probe, and light intensity probe, and transmit the monitoring parameters to the touch display in real time. The combined LED multi-color light board is placed inside the algae constant temperature pool, and the dimmer controls the LED light source intensity and adjusts the light source type. The LED matrix-distributed lamp beads provide blue light / red light / green light / white light / yellow light / purple light for algae culture.

[0013] The above algae intelligent constant temperature culture system is operated according to the following steps: 1. Turn on the power switch to put the device into operation / standby state; 2. Inject coolant into the constant temperature water tank of the equipment to the required water level; 3. Inject culture solution into the algae culture vessel until the amount required for the experiment; 4. The inlet / outlet of the thermostatic unit is connected to the inlet / outlet of the thermostatic pool; 5. Set the constant temperature water tank temperature on the touch display; 6. Set the required light intensity and type through the dimmer; 7. Turn on the circulating water system; 8. Start the equipment and observe the operating status; 9. The intelligent air supply device is turned on, the interval time is set, and the ratio of air and carbon dioxide is adjusted; 10. PH, conductivity, dissolved oxygen, temperature, light and other probes are inserted into the algae culture vessel to monitor and collect values.

[0014] This equipment uses an external cold source to cool the algae culture device through a constant temperature water tank to maintain a constant temperature. First, the equipment uses a constant temperature water tank to reduce the coolant to the required temperature, and then circulates the cooling to the algae constant temperature pool through a circulating water pump. The advantage of this approach is that it will not cause a large cold and hot shock to the culture sample and affect its growth. When the intelligent control unit determines that gas replenishment is needed, the gas replenishment device automatically starts. The air pump works to increase the pressure and flow of the mixed gas, and the carbon dioxide cylinder releases the corresponding gas. After mixing in the gas mixer, the mixed gas is accurately passed into each algae culture device through a 6-way gas valve to improve the growth efficiency of the algae. The advantage is that different algae have different sensitivity to carbon dioxide concentration. The system can combine the real-time growth parameter status of the algae to intelligently adjust the time, flow rate and other parameters of the gas replenishment to ensure that the most suitable gas replenishment effect is achieved, which promotes the growth rate of algae culture without affecting the cell morphology or growth inhibition.

[0015] In addition, the traditional incubator heats the temperature in the culture bottle by adjusting the air temperature, which is air contact. This device uses an external cold source to cool the algae culture device through a constant temperature water tank to maintain a constant temperature, which will not cause a large cold and hot shock to the culture sample and is conducive to the growth of algae.

[0016] The same type of microalgae was selected and the same nutrient conditions were used. The initial algae density (5×10 4 cells / mL), and algae culture experiments were carried out in this device and in a traditional incubator. The results showed that the cell density of microalgae in this device was 1367±46×10 4 cells / mL, the traditional incubator is 745±42×10 4 cells / mL. During the entire culture period, the average specific growth rate of the microalgae in this device was 0.400 day -1 , while the traditional incubator is 0.357 day -1 .like Figure 5 shown.

[0017] The technical solution provided by the present invention is introduced in detail above. For those skilled in the art, according to the concept of the embodiments of the present invention, there may be changes in the specific implementation method and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. An intelligent constant temperature culture system for algae, characterized in that: It includes an intelligent control unit, a constant temperature circulating water unit, and an algae cultivation unit; the intelligent control unit is used to intelligently control the temperature of the constant temperature circulating water unit so that the water temperature entering the algae cultivation unit is constant. At the same time, the algae cultivation unit is built with various water quality parameter probes, which are used to transmit the real-time detection parameters of the algae cultivation stage back to the intelligent control unit for data storage and real-time display.

2. The algae intelligent constant temperature culture system according to claim 1, characterized in that: The intelligent control unit includes a touch display, an intelligent air replenishment device, and an electrical compartment; The touch display controls and adjusts the temperature of the constant temperature circulating water unit through the PLC master control unit in the electrical compartment to ensure that the water temperature entering the algae cultivation unit through the circulating water inlet is constant; at the same time, the touch display receives the pH, conductivity, dissolved oxygen, temperature, and light parameters monitored in real time by various probes in the algae cultivation unit, and the collector in the electrical compartment collects and transmits the monitored parameters in real time to the touch display to generate a parameter change curve; the touch display controls and operates the PLC master control unit in the electrical compartment to adjust the intelligent gas replenishment device to replenish the carbon dioxide mixed gas to the algae cultivation unit; The intelligent air replenishment device includes a gas mixer, an air pump, a carbon dioxide cylinder, and a 6-way gas distribution valve for mixed gas; the gas mixer is connected to the air pump and the carbon dioxide cylinder, and the intelligent control unit sets the ventilation flow and ventilation time to control the operation of the air pump. The air is compressed by the air pump, mixed with carbon dioxide and introduced into the gas mixer; the mixed gas is transported to the 6-way gas distribution valve for mixed gas through the mixed gas outlet interface under the action of the air pump pressure, and connected to different algae culture vessels as needed.

3. The algae intelligent constant temperature culture system according to claim 1, characterized in that: The constant temperature circulating water unit includes a constant temperature unit, a constant temperature water tank, and a circulating water device; The constant temperature unit compresses the refrigerant into high-temperature and high-pressure gas by the compressor according to the temperature set by the intelligent control unit, enters the condenser to condense into liquid, passes through the drying filter to remove impurities, and then flows into the evaporator. The liquid refrigerant quickly vaporizes and absorbs heat, and the temperature of the constant temperature water tank is adjusted to ensure constant; The constant temperature water tank is equipped with a heating rod and a water level sensor. When the temperature is lower than the set value, the heating rod operates. When the temperature is higher than the set value, the heating rod is in standby mode. The water level sensor is used to accurately measure the water level and provide real-time feedback to the touch display. When the water level is lower than the minimum value, the intelligent control unit reminds manual water replenishment. The circulating water device is composed of a water pump and a circulating pipe body. The water pump transports circulating water with constant temperature to the algae cultivation unit through the circulating pipe body to keep the temperature in the algae constant temperature pool stable.

4. The algae intelligent constant temperature culture system according to claim 1, characterized in that: The algae cultivation unit includes an algae constant temperature pool, an algae incubator, a combined LED multi-color light panel, and a monitoring probe; The algae constant temperature pool is a transparent water tank made of acrylic sheet, with a built-in algae incubator, and a circulating water device to keep the temperature constant; The algae culture device comprises a plurality of detachable algae culture test tubes, and a built-in ventilation pipe for connecting a 6-way gas distribution valve to realize intelligent gas replenishment; The monitoring probes include a temperature probe, a pH probe, a conductivity probe, a dissolved oxygen probe and / or a light intensity probe, which transmit the monitoring parameters to the touch display in real time; The combined LED multi-color light panel is placed inside the algae constant temperature pool. The dimmer controls the LED light source intensity and adjusts the light source type. The lamp beads distributed in the LED matrix provide light of different colors for algae cultivation.

5. The method for operating the intelligent constant temperature culture system for algae according to any one of claims 1 to 4, characterized in that: Follow these steps:

1. Turn on the power switch to put the device into operation / standby state; 2. Inject coolant into the constant temperature water tank of the equipment to the required water level; 3. Inject culture solution into the algae culture vessel until the amount required for the experiment; 4. The inlet / outlet of the thermostatic unit is connected to the inlet / outlet of the thermostatic pool; 5. Set the constant temperature water tank temperature on the touch display; 6. Set the required light intensity and type through the dimmer; 7. Turn on the circulating water system; 8. Start the equipment and observe the operating status; 9. The intelligent air supply device is turned on, the interval time is set, and the ratio of air and carbon dioxide is adjusted; 10. The pH probe, conductivity probe, dissolved oxygen probe, temperature probe and / or light probe are inserted into the algae culture vessel to monitor and collect values.

Images