Salt-tolerant growth-promoting bacterium strain D5-2 and application thereof

A technology of promoting growth bacteria and strains, applied in the field of microorganisms, to achieve the effects of reducing use, improving salt tolerance, and increasing plant type

Active Publication Date: 2019-08-06
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research work on PGPR improving saline soil and promoting plant salt tolerance is still in its infancy, mainly focusing on Pseudomonas spp., Bacillus spp., etc., and their role in adjusting plant Some mechanistic discussions have been made on the balance of endogenous hormones, ion homeostasis, anti-oxidation and photosynthesis
However, there are few introductions about the salt-tolerant rhizosphere growth-promoting bacteria of tomato

Method used

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  • Salt-tolerant growth-promoting bacterium strain D5-2 and application thereof
  • Salt-tolerant growth-promoting bacterium strain D5-2 and application thereof
  • Salt-tolerant growth-promoting bacterium strain D5-2 and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0015] Example 1 Isolation, screening and identification of salt-tolerant growth-promoting bacterial strain D5-2

[0016] 1. Isolation and screening of salt-tolerant growth-promoting bacteria strain D5-2:

[0017] From the Zhangjiagang greenhouse, the soil samples were collected from the rhizosphere soil of the pine cauliflower, and 2.0g of the rhizosphere soil samples of the pine cauliflower (with the plant root system) were weighed and placed in a conical flask with 100mL of double distilled water, sealed with a sealing film, and placed in a 200r Shake at a constant temperature of 30°C for 24 hours in a shaker / min to obtain a soil suspension. Take 1mL of soil suspension and transfer it to 100mL LB liquid medium containing 2% NaCl, incubate at a constant temperature of 30°C at 200r / min for 24 hours, and then transfer it again to 4% NaCl containing In the LB culture medium, repeat the above steps until the NaCl content in the LB culture medium reaches 6%. The resulting bacte...

Embodiment 2

[0027] Example 2 Salt-tolerant growth-promoting bacterial strain D5-2 to tomato salt-tolerant growth-promoting test

[0028] Preparation of bacterial agent containing salt-tolerant growth-promoting bacteria strain D5-2: inoculate D5-2 in LB liquid medium, culture at 180rpm, 30°C until OD600 is 1, then centrifuge the bacterial solution at 6000rpm for 10min, discard The supernatant was reinjected into an equal volume of dd water, shaken and resuspended, centrifuged at 6000rpm for 10 minutes, the supernatant was discarded, and repeated twice, and the bacterial agent was obtained after resuspension.

[0029] Experimental design: select plump tomato seeds of the same size and sterilize the surface with 5% NaClO solution for 10 minutes, rinse the seeds repeatedly with deionized water for several times, soak the seeds in deionized water for 24 hours, and then place them in a culture medium covered with moist gauze. Germinate on a dish at 27°C for 2 days. The seeds with the same bud ...

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Abstract

The invention relates to a salt-tolerant growth-promoting bacterium strain D5-2. The salt-tolerant growth-promoting bacterium strain D5-2 is preserved in the China General Microbiological Culture Collection Center on the preservation date of 5 May 2019 with the preservation number being CGMCC NO.17706, and the 16S rDNA nucleotide sequence of the salt-tolerant growth-promoting bacterium strain D5-2is as shown in SEQID NO.1. When the strain D5-2 is inoculated, under salt stress, the salt-tolerant growth promoting capacity of solanum lycopersicum can be notably improved. The strain is prepared into a microbial inoculum, and then the microbial inoculum is injected into rhizosphere soil of solanum lycopersicum seedlings, so that the plant type, the plant height and the stem thickness of the solanum lycopersicum can be obviously enlarged, the dry matter accumulation of the solanum lycopersicum seedlings can be notably promoted, the microbial inoculum has promoting effects on the root length, the surface area, the root volume and the number of root tips of the solanum lycopersicum seedlings, a salt injury phenomenon is alleviated, the growth of the solanum lycopersicum seedlings is promoted, and increase of salt tolerance of the solanum lycopersicum is facilitated.

Description

technical field [0001] The invention relates to a salt-tolerant growth-promoting bacterial strain D5-2 and its application, belonging to the technical field of microorganisms. Background technique [0002] Tomato (Solanum lycopersicum) is the most planted vegetable crop of Solanaceae after potato in the world, and it is also an important model plant of succulent fruit and Solanaceae (Salvalaggio et al., 2017; Zhang Tianpeng and Yang Xinghong, 2018). Welcome, there are more than 3,000 tomato varieties reported worldwide, and global tomato production is facing serious challenges from biotic and abiotic stresses. Salinity is a major environmental stress in agriculture that inhibits crop growth and reduces food yields to limit the productivity of major food crops worldwide (Kaushal et al., 2016; Li Lulu et al., 2019). Tomatoes are moderately salt sensitive and are mainly grown in warm and arid regions of the world where soils tend to be relatively salinity. With the increasing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 赵海燕陈军郑青松兰汝佳韩鹰吴馨怡
Owner NANJING AGRICULTURAL UNIVERSITY
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