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A kind of salt-tolerant growth-promoting bacterial strain b9 and its application

A technology that promotes growth and salt tolerance, applied in the field of microorganisms, to achieve the effects of improving salt tolerance, increasing plant type, and slowing down the phenomenon of salt damage

Active Publication Date: 2022-03-11
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research work on PGPR improving saline soil and promoting plant salt tolerance is still in its infancy, mainly focusing on Pseudomonas spp., Bacillus spp., etc., and their role in adjusting plant Some mechanistic discussions have been made on the balance of endogenous hormones, ion homeostasis, anti-oxidation and photosynthesis
However, there are few introductions about the salt-tolerant rhizosphere growth-promoting bacteria of tomato

Method used

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  • A kind of salt-tolerant growth-promoting bacterial strain b9 and its application
  • A kind of salt-tolerant growth-promoting bacterial strain b9 and its application
  • A kind of salt-tolerant growth-promoting bacterial strain b9 and its application

Examples

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Effect test

Embodiment 1

[0015] Example 1 Isolation, screening and identification of salt-tolerant growth-promoting bacterial strain B9

[0016] 1. Isolation and screening of salt-tolerant growth-promoting bacteria strain B9:

[0017] Weigh 2.0g of Salicornia rhizosphere soil sample (with plant root system) and place it in a conical flask filled with 100mL of double distilled water, seal it with a parafilm, shake it in a shaker at 200r / min at 30°C for 24h at a constant temperature, that is, Get a soil suspension. Take 1mL of soil suspension and transfer it to 100mL LB liquid medium containing 2% NaCl, culture at 30°C at 200 r / min for 24 hours, then transfer again to 4% NaCl at an inoculum size of 1:100 Cultured in the LB medium for 24 hours, and repeated the above steps until the NaCl content in the LB culture medium reached 6%. The resulting bacterial culture was 10 6 Dilute and spread, and pick a single colony with good growth to obtain the salt-tolerant growth-promoting bacteria strain B9.

[0...

Embodiment 2

[0026] Example 2 Salt-tolerant and growth-promoting test of salt-tolerant growth-promoting bacteria strain B9 on tomato

[0027] Preparation of tomato salt-tolerant growth promoter: B9 was inoculated in LB liquid medium, cultivated at 180rpm and 30°C until OD600 was 1 (i.e. 10 8 CFU / mL), to obtain the bacterial solution, centrifuge the bacterial solution at a speed of 6000rpm for 10min, discard the supernatant, then inject an equal volume of dd water, shake and resuspend, centrifuge at a speed of 6000rpm for 10min, discard the supernatant, repeat 2 After resuspension, the tomato salt tolerance growth promoter is obtained.

[0028]Experimental design: select plump tomato seeds of the same size and sterilize the surface with 5% NaClO solution for 10 minutes, rinse the seeds repeatedly with deionized water for several times, soak the seeds in deionized water for 24 hours, and then place them in a culture medium covered with moist gauze. Germinate on a dish at 27°C for 2 days. ...

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Abstract

The present invention relates to a salt-tolerant growth-promoting bacteria strain B9, which is preserved in the General Microbiology Center of the China Microorganism Culture Collection and Administration Commission with a preservation date of May 5, 2019 and a preservation number of CGMCC NO.17708. The 16S rDNA nucleotide sequence of the salt-tolerant growth-promoting bacteria strain B9 is shown in SEQ ID NO.1. The salt-tolerant growth-promoting bacteria strain B9 can significantly improve the salt-tolerant growth-promoting ability of tomato under salt stress. The strain is made into a tomato salt-tolerant growth-promoting agent, and then injected into the rhizosphere soil of tomato seedlings, which can significantly increase the tomato growth-promoting ability. Plant type, plant height and stem diameter significantly promote the accumulation of dry matter in tomato seedlings, and promote the root length, surface area, root volume, and root tip number of tomato seedlings, slow down the phenomenon of salt damage, and promote tomato seedlings. The growth of tomato helps to improve the salt tolerance of tomatoes and reduce the use of chemical fertilizers.

Description

technical field [0001] The invention relates to a salt-tolerant growth-promoting bacterial strain B9 and an application thereof, belonging to the technical field of microorganisms. Background technique [0002] Tomato (Solanum lycopersicum) is the most planted vegetable crop of Solanaceae after potato in the world, and it is also an important model plant of succulent fruit and Solanaceae (Salvalaggio et al., 2017; Zhang Tianpeng and Yang Xinghong, 2018). Welcome, there are more than 3,000 tomato varieties reported worldwide, and global tomato production is facing serious challenges from biotic and abiotic stresses. Salinity is a major environmental stress in agriculture that inhibits crop growth and reduces food yields to limit the productivity of major food crops worldwide (Kaushal et al., 2016; Li Lulu et al., 2019). Tomatoes are moderately salt sensitive and are mainly grown in warm and arid regions of the world where soils tend to be relatively salinity. With the incre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/27A01P21/00C12R1/38
CPCC12N1/20A01N63/00C12R2001/38C12N1/205
Inventor 郑青松兰汝佳陈军赵海燕韩鹰范翠枝
Owner NANJING AGRICULTURAL UNIVERSITY
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