A kind of salt-tolerant growth-promoting bacterial strain d5-2 and its application

A technology of growth-promoting bacteria and strains, applied in the field of microorganisms, to achieve the effects of promoting root tip number, improving salt-tolerant growth-promoting ability, and improving salt-tolerance

Active Publication Date: 2021-09-24
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the research work on PGPR improving saline soil and promoting plant salt tolerance is still in its infancy, mainly focusing on Pseudomonas spp., Bacillus spp., etc., and their role in adjusting plant Some mechanistic discussions have been made on the balance of endogenous hormones, ion homeostasis, anti-oxidation and photosynthesis
However, there are few introductions about the salt-tolerant rhizosphere growth-promoting bacteria of tomato

Method used

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  • A kind of salt-tolerant growth-promoting bacterial strain d5-2 and its application
  • A kind of salt-tolerant growth-promoting bacterial strain d5-2 and its application
  • A kind of salt-tolerant growth-promoting bacterial strain d5-2 and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0015] Example 1 Isolation, screening and identification of salt-tolerant growth-promoting bacterial strain D5-2

[0016] 1. Isolation and screening of salt-tolerant growth-promoting bacteria strain D5-2:

[0017] From the Zhangjiagang greenhouse, the soil samples were collected from the rhizosphere soil of the pine cauliflower, and 2.0g of the rhizosphere soil samples of the pine cauliflower (with the plant root system) were weighed and placed in a conical flask with 100mL of double distilled water, sealed with a sealing film, and placed in a 200r Shake at a constant temperature of 30°C for 24 hours in a shaker / min to obtain a soil suspension. Take 1mL of soil suspension and transfer it to 100mL LB liquid medium containing 2% NaCl, incubate at a constant temperature of 30°C at 200r / min for 24 hours, and then transfer it again to 4% NaCl containing In the LB culture medium, repeat the above steps until the NaCl content in the LB culture medium reaches 6%. The resulting bacte...

Embodiment 2

[0027] Example 2 Salt-tolerant growth-promoting bacterial strain D5-2 to tomato salt-tolerant growth-promoting test

[0028] Preparation of bacterial agent containing salt-tolerant growth-promoting bacteria strain D5-2: inoculate D5-2 in LB liquid medium, culture at 180rpm, 30°C until OD600 is 1, then centrifuge the bacterial solution at 6000rpm for 10min, discard The supernatant was reinjected into an equal volume of dd water, shaken and resuspended, centrifuged at 6000rpm for 10 minutes, the supernatant was discarded, and repeated twice, and the bacterial agent was obtained after resuspension.

[0029] Experimental design: select plump tomato seeds of the same size and sterilize the surface with 5% NaClO solution for 10 minutes, rinse the seeds repeatedly with deionized water for several times, soak the seeds in deionized water for 24 hours, and then place them in a culture medium covered with moist gauze. Germinate on a dish at 27°C for 2 days. The seeds with the same bud ...

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Abstract

The present invention relates to a salt-tolerant growth-promoting bacteria strain D5‑2, which is preserved in the General Microbiology Center of China Microbial Strain Preservation Management Committee. The preservation date is May 5, 2019, and the preservation number is CGMCC NO.17706. The 16S rDNA nucleotide sequence of the salt-promoting bacterial strain D5‑2 is shown in SEQ ID NO.1. The inoculation of strain D5‑2 can significantly improve the salt-tolerant growth-promoting ability of tomato under salt stress. The strain is made into a bacterial agent, and then injected into the root soil of tomato seedlings, which can significantly increase the plant type, plant height and stem of tomato. Coarse, significantly promotes the dry matter accumulation of tomato seedlings, and promotes the root length, surface area, root volume, and root tip number of tomato seedlings, slows down the phenomenon of salt damage, thereby promoting the growth of tomato seedlings, and helping to improve Salt tolerance of tomato.

Description

technical field [0001] The invention relates to a salt-tolerant growth-promoting bacterial strain D5-2 and its application, belonging to the technical field of microorganisms. Background technique [0002] Tomato (Solanum lycopersicum) is the most planted vegetable crop of Solanaceae after potato in the world, and it is also an important model plant of succulent fruit and Solanaceae (Salvalaggio et al., 2017; Zhang Tianpeng and Yang Xinghong, 2018). Welcome, there are more than 3,000 tomato varieties reported worldwide, and global tomato production is facing severe challenges from biotic and abiotic stresses. Salinity is a major environmental stress in agriculture that inhibits crop growth and reduces food yields to limit the productivity of major food crops worldwide (Kaushal et al., 2016; Li Lulu et al., 2019). Tomatoes are moderately salt sensitive and are mainly grown in warm and arid regions of the world where soils tend to be relatively salinity. With the increasing ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P21/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 赵海燕陈军郑青松兰汝佳韩鹰吴馨怡
Owner NANJING AGRICULTURAL UNIVERSITY
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