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A Microbacillus sinensis strain sm8 and its application in salt-tolerant growth promotion

A technology of Microbacterium sinensis and Microbacteria, applied in the direction of microorganism-based methods, applications, microorganisms, etc., can solve the problems of poor adaptability and poor effect of related microorganisms, so as to promote crop growth, increase germination rate and growth rate, and promote significant effect

Active Publication Date: 2022-07-12
INST OF SOIL & FERTILIZER XINJIANG ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although a large number of plant growth-promoting bacteria have been discovered at home and abroad, due to the arid environment and diverse salinity in Xinjiang, there are problems of poor adaptability and poor effect of related microorganisms.

Method used

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  • A Microbacillus sinensis strain sm8 and its application in salt-tolerant growth promotion
  • A Microbacillus sinensis strain sm8 and its application in salt-tolerant growth promotion
  • A Microbacillus sinensis strain sm8 and its application in salt-tolerant growth promotion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Isolation, Screening, Purification and Preservation of Microbacillus sinensis SM8 CGMCC No.15757 Strain

[0031] The rhizosphere soil samples of halophytes in Heshuo area of ​​Xinjiang were collected, and the microorganisms were isolated and purified by gradient dilution method. Weigh 10 g of soil sample, put it into 100 ml of sterile water (add glass beads), shake at 200 rpm, 30° C. for 20 min to fully disperse the sample. After serial dilution, dilute with sterile water to prepare 10 -2 -10 -5 sample suspension diluent. Take 100 μL of the dilutions of each concentration, spread them on a TSA solid medium plate containing 2% NaCl by conventional coating method, and place them in a 30° C. constant temperature incubator for cultivation. After 48 hours, after the colonies have grown on the plate, a single colony on the plate is picked for purification and culture. For purified single colonies ( figure 1 ), transferred to the slant of TSA medium containing 2...

Embodiment 2

[0032] Example 2 Identification of Microbacillus sinensis SM8 CGMCC No.15757 strain

[0033] Sequence determination and analysis of 16S rDNA of a new strain of Microbacillus sinensis CGMCC No.15757:

[0034] (1) Extraction of PCR template DNA:

[0035] The purified strain IS7 was inoculated into 2% NaCl TSA medium, cultured on a shaker at 30°C for 2 days, the bacteria were collected, and the total genomic DNA was extracted using a DNA extraction kit.

[0036] (2) PCR amplification

[0037] Use specific primers:

[0038] 27F:5'-AGAGTTTGATCCTGGCTCAG-3',

[0039] 1492R:5'-GGTTACCTTGTTACGACTT-3';

[0040] The total volume of the PCR reaction system was 25 μL, and the PCR amplification conditions were as follows: 94 °C for 5 min; 94 °C for 45 s, 56 °C for 45 s, 72 °C for 45 s, 30 cycles; 72 °C for 10 min.

[0041] (3) Sequence determination

[0042] The PCR amplification product was sequenced after electrophoresis detection and purification, and a 16S r DNA sequence with a le...

Embodiment 3

[0045] Example 3 Detection of salt tolerance and growth-promoting ability of Microbacillus sinensis SM8 CGMCC No.15757 strain

[0046] After adding 10% NaCl to the TSA medium, the plate was poured and inoculated with Microbacillus sinensis SM8 CGMCC No.15757. Incubate at 30°C, and observe the growth of the bacteria for 2-5d.

[0047] Microbacillus sinensis SM8 CGMCC No. 15757 was inoculated on phosphate solubilizing (inorganic phosphorus and organic phosphorus) bacterial medium at 30°C, and the size of the transparent circle was observed after culturing for one week.

[0048] Microbacillus sinensis SM8 CGMCC No. 15757 was inoculated into TSA liquid medium containing L-tryptophan (100mg / L) and 2% NaCl, and after culturing on a shaking table (30°C, 180r / min) for 1 d, 50 μL of bacteria were taken. The suspension was dropped onto a white ceramic plate, and an equal volume of Salkowski colorimetric solution (50 mL of 35% HClO) was added at the same time. 4 +1mL 0.5mol / L FeCl 3 )...

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Abstract

The invention provides a new strain of Microbacter sinensis SM8 CGMCC No.15757, which has the characteristics of growing under both low temperature and high temperature conditions, is resistant to high salt and high pH, ​​and can convert insoluble phosphorus in soil to be directly absorbed by plants , the utilization of available phosphorus, and has the ability to synthesize indole acetic acid, produce ACC deaminase, and play a role in promoting plant growth. The use of related inoculants can significantly improve the germination rate and growth rate of cotton under salt stress, and have obvious salt tolerance and growth-promoting effects. It can not only reduce the amount of chemical fertilizers, but also save farmers' expenses, increase economic income, and have good ecological and social benefits.

Description

Technical field: [0001] The invention relates to a Microbacillus sinensis SM8 with salt-tolerant growth-promoting effect and its application in improving the salt stress tolerance of crops and promoting the growth of crops in seedling stage, belonging to the technical field of microbial strains and application thereof. Background technique: [0002] Saline-alkali soil is a general term for soils with different degrees of salinization and alkalization. Among them, the soil with a soluble salt content of more than 0.6-2% in the topsoil layer is called saline soil; there are many exchangeable sodium ions in the soil colloid (alkaline degree of 15% or 20%), which is an alkaline reaction (pH 8.5-11.0 ) soils are called alkaline soils. According to incomplete statistics of UNESCO and FAO, the area of ​​saline-alkali land in the world is 950 million hectares. Among them, my country accounts for more than 10% of the world, and the area of ​​saline-alkali land reaches 99 million he...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01G7/06C12R1/01
CPCA01G7/06C12R2001/01C12N1/205Y02A40/22
Inventor 徐万里楚敏张志东顾美英朱静唐琦勇唐光木宋素琴
Owner INST OF SOIL & FERTILIZER XINJIANG ACAD OF AGRI SCI
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