Method for collection and extraction of disease-resistant rootstock root exudate

A technology for root exudates and rootstocks, which can be applied in botanical equipment and methods, chemicals for biological control, animal repellents, etc. The operation steps are simple and easy to operate, reduce root damage, and alleviate the effect of high incidence of bacterial wilt

Inactive Publication Date: 2019-05-07
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, due to continuous cropping and intensified salinization of soil, bacterial wilt is very easy to spread in large areas under high temperature and rainy environmental conditions, often causing major agricultural economic losses. Conventional chemical agents and biological control methods are difficult to cure

Method used

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  • Method for collection and extraction of disease-resistant rootstock root exudate

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The collection and extraction of the root exudates of the rootstock "Fanzhen No. 1" are carried out as follows:

[0028] (1) 50-hole plug trays (62mm×30mm) were used for seedling cultivation of the tested species, and the seedling-raising substrate was prepared from the following raw materials in parts by weight: 15 parts of peat, 5 parts of coconut bran, 5 parts of perlite, 1 part of vermiculite and mushroom residue 15 parts of fermented product;

[0029] (2) After sowing, when the seedling grows to three leaves and one heart, it is transferred to a nutrient bowl (80mm × 65mm) for cultivation, and the substrate is consistent with the step 1, and the water and fertilizer management is carried out uniformly;

[0030] (3) After the seedlings grow to the budding stage, the root exudates are collected, and 10 strong seedlings whose growth is basically the same are taken, and the seedlings are carefully taken out from the nutrient bowl, and the roots are avoided as much as p...

Embodiment 2

[0035] The rootstock "Eggplant No. 21" is collected and extracted, and the implementation method is as follows:

[0036] (1) 50-hole plug trays (62mm×30mm) were used for seedling cultivation of the tested varieties, and the seedling-raising substrate was prepared from the following raw materials in parts by weight: 20 parts of peat, 15 parts of coconut bran, 15 parts of perlite, 5 parts of vermiculite and mushroom residue 5 parts of fermented product;

[0037] (2) After sowing, when the seedling grows to three leaves and one heart, it is transferred to a nutrient bowl (80mm × 65mm) for cultivation, and the substrate is consistent with the step 1, and the water and fertilizer management is carried out uniformly;

[0038] (3) After the seedlings grow to the budding stage, the root exudates are collected, and 10 strong seedlings whose growth is basically the same are taken, and the seedlings are carefully taken out from the nutrient bowl, and the roots are avoided as much as poss...

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Abstract

Relating to the field of disease-resistant cultivation techniques of vegetables and plant metabolite extraction, the invention discloses a method for collection and extraction of disease-resistant rootstock root exudate. The method includes: seedling raising of test solanum lycopersicum rootstock variety, hydroponic collection, extraction and concentration, thus obtaining the plant root exudate. The test solanum lycopersicum rootstock variety solanum lycopersicum rootstock No.1 and/or solanum lycopersicum rootstock No.21. The method provided by the invention is simple in operation, safe and environment-friendly, does not destroy the plant itself, and the obtained root exudate has high purity, and can inhibit the bioactivity of soil ralstonia solanacearum when applied in practical production, thus alleviating the problem of high incidence of ralstonia solanacearum in solanum lycopersicum producing areas.

Description

technical field [0001] The invention relates to the technical field of vegetable metabolite extraction and disease-resistant cultivation, in particular to a method for collecting and extracting root exudates of disease-resistant rootstocks. Background technique [0002] Bacterial wilt is a soil-borne vascular disease caused by Ralstonia solanacearum E.F.Smith (hereinafter referred to as Ralstonia solanacearum), which commonly occurs in the south of the Yangtze River in my country, especially in South China. The damage is most serious on crops. Tomato (Lycopersicon esculentum Mill.) is rich in nutrition and high in yield. It is one of the main vegetable varieties in my country. In recent years, due to continuous cropping and increased salinization of soil, bacterial wilt is very easy to spread in a large area under high temperature and rainy environmental conditions, often causing major agricultural economic losses, and conventional chemical agents and biological control meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/38A01P1/00A01G31/00
Inventor 于文进赵文宗阳燕娟钟川
Owner GUANGXI UNIV
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