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Lactobacillus fermentium and application of lactobacillus fermentium for preventing and treating plant fungal disease

A technology for fermenting lactobacillus and lactic acid bacteria, applied to lactic acid bacteria and its application field in the prevention and control of plant fungal diseases

Active Publication Date: 2015-07-01
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the extensive use of chemical fertilizers, pesticides and other agricultural chemicals can greatly increase the production of agricultural products, it also brings many adverse effects

Method used

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  • Lactobacillus fermentium and application of lactobacillus fermentium for preventing and treating plant fungal disease
  • Lactobacillus fermentium and application of lactobacillus fermentium for preventing and treating plant fungal disease
  • Lactobacillus fermentium and application of lactobacillus fermentium for preventing and treating plant fungal disease

Examples

Experimental program
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Effect test

example 1

[0032] (1) Strain culture

[0033] Lactobacillus fermentum CGMCC No.8735 was inoculated in the solid medium and cultured at 30°C for 12h. Choose a bacterium colony from the solid medium, pick the thalline, insert in the 250mL Erlenmeyer flask that the seed liquid culture medium is housed, wherein the volume of the seed liquid culture medium is 16% of the capacity of the Erlenmeyer flask (250mL Erlenmeyer flask contains Into 40mL seed liquid culture medium), rotate speed 100r / min, cultivate 10h under the condition of 30 ℃ to obtain seed liquid; take the seed liquid and put it into a 250mL Erlenmeyer flask equipped with fermentation medium, wherein the volume of fermentation medium is the capacity of the Erlenmeyer flask 16% (250mL Erlenmeyer flask filled with 40mL seed liquid medium), the volume ratio of seed liquid to fermentation medium was 1:100, the rotation speed was 120r / min, and cultured at 30°C for 28h to obtain the fermentation liquid.

[0034] The above solid medium ...

example 2

[0053] (1) Strain culture

[0054] Lactobacillus fermentum CGMCC No.8735 was inoculated in solid medium and cultured at 36°C for 18h. Select a bacterium colony from the solid medium, pick the thalline, and insert it into a 250mL conical flask equipped with a seed liquid medium, wherein the volume of the seed liquid medium is 32% of the capacity of the conical flask, and the rotating speed is 140r / min, 36 Cultivate under the condition of ℃ for 16h to obtain seed solution; take the seed solution and insert it into a 250mL Erlenmeyer flask equipped with fermentation medium, wherein the volume of the fermentation medium is 32% of the capacity of the Erlenmeyer flask, and the volume ratio of the seed solution to the fermentation medium is 1:20, rotation speed 140r / min, cultured at 36°C for 28h to obtain fermentation broth.

[0055] The above solid medium components are (g / L): peptone 10, beef extract 10, yeast extract 5, glucose 20, disodium hydrogen citrate 2, sodium acetate 5, d...

example 3

[0074] (1) Strain culture

[0075] Lactobacillus fermentum CGMCC No.8735 was inoculated in solid medium and cultured at 40°C for 24h. Select a colony from the solid medium, pick the thalline, and insert it into a 250mL Erlenmeyer flask containing the seed liquid medium, wherein the seed liquid medium is 40% of the capacity of the Erlenmeyer flask, the speed is 180r / min, 40°C Cultivate under conditions for 22 hours to obtain seed liquid; take the seed liquid and insert it into a 250mL Erlenmeyer flask equipped with a fermentation medium, wherein the volume of the fermentation medium is 40% of the capacity of the Erlenmeyer flask, and the volume ratio of the seed liquid to the fermentation medium is 1 : 10, rotating speed 160r / min, cultured at 40°C for 36h to obtain fermentation broth.

[0076] The above solid medium components are (g / L): peptone 11, beef extract 11, yeast extract 5.5, glucose 22, disodium hydrogen citrate 2.5, sodium acetate 5.5, dipotassium hydrogen phosphate...

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Abstract

The invention discloses lactobacillus fermentium and an application of lactobacillus fermentium for preventing and treating a plant fungal disease and belongs to the field of biocontrol agents. The bacterial strain is obtained by separating from a soil planted with tomatoes and the classification name is lactobacillus fermentium; the Latin name of the strain is Lactobacillus fermentium, the reference microorganism is NJHYHWGY20130877, the preservative date is 17th, January, 2014, and the accession number in the preservative center is CGMCC No.8735. The lactobacillus fermentium is fermented and cultured to obtain fermentation liquor, the fermentation liquor is condensed to prepare the lactein concentrated liquor, and the concentrated liquor is diluted and sprayed on the crop leaf surface; and the bacterial strain has good effect for inhibiting the crop fungal disease, simultaneously has the functions of promoting the crop growth and meets ecological agriculture requirement.

Description

technical field [0001] The invention belongs to the field of biological control preparations, and in particular relates to a lactic acid bacterium and its application in the prevention and treatment of plant fungal diseases. technical background [0002] In today's society, the sustainable development of agriculture has become the leading trend in most countries in the world. As the primary issue of economic and social development, agricultural issues have been put on the agenda of all countries. As a large agricultural country, China's agricultural production safety concerns the national economy, people's livelihood and social harmony and stability. Although the extensive use of chemical fertilizers, pesticides and other agricultural chemicals can greatly increase the production of agricultural products, it also brings many adverse effects. In response to this problem, green and eco-friendly biopesticides have emerged in my country one after another. Compared with traditi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04A01N63/02A01P3/00C12R1/225
CPCC12P1/04A01N63/10C12R2001/225C12N1/205
Inventor 胡永红梁萌萌杨文革刘翔曹翠翠刘邮洲
Owner NANJING UNIV OF TECH
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