The invention relates to the technical field of biological control of plant diseases, and discloses a virus-carrying low-toxic strain of Sclerotinia sclerotiorum and its application in biological control. The strain is Sclerotinia 767R45, and the preservation number is: CCTCC NO: M2020419 . Volatile substances produced by S. sclerotiorum 767R45 strain and 10% fermentation filtrate can inhibit S. sclerotiorum, Rhizoctonia solani, Botrytis cinerea, Fusarium oxysporum, Helminthes oryzae, and black shank of rape and mycelial growth of Sour rot citri. In addition, the volatile substances and fermentation filtrate produced by Sclerotinia 767R45 can inhibit the occurrence of gray mold, penicillium and green mold during storage, and the fermentation filtrate can also inhibit the occurrence of Sclerotinia sclerotiorum, and its volatile Substances can promote the growth of Arabidopsis seedlings. The invention turns plant pathogenic bacteria into beneficial biological control resources, and provides a new perspective and new means for the biological control of crop fungal diseases.