Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Aspergillus terreus strain SYAT-1 tolerant to saline and alkaline and resistant to pathogenic fungi and application of aspergillus terreus strain SYAT-1

A technology of SYAT-1 and Aspergillus terreus, applied in the field of microbiology, can solve the problem that Aspergillus terreus is not found to resist plant pathogenic fungi

Active Publication Date: 2021-08-10
JILIN UNIV
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the resistance of Aspergillus terreus to plant pathogenic fungi

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Aspergillus terreus strain SYAT-1 tolerant to saline and alkaline and resistant to pathogenic fungi and application of aspergillus terreus strain SYAT-1
  • Aspergillus terreus strain SYAT-1 tolerant to saline and alkaline and resistant to pathogenic fungi and application of aspergillus terreus strain SYAT-1
  • Aspergillus terreus strain SYAT-1 tolerant to saline and alkaline and resistant to pathogenic fungi and application of aspergillus terreus strain SYAT-1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. Isolation and identification of Aspergillus terreus strain SYAT-1

[0029] Isolation of the strains: The soil samples for the isolation of the strains were from Songyuan City, Jilin Province. Select the land with high salinization, take the soil below 2cm of the soil surface, put it into a sterilized glass bottle and bring it back to the laboratory for later use. Using the dilution coating method, the diluted and clarified soil supernatant was spread on the PDA medium, and a fungal strain was obtained through screening, isolation and purification.

[0030] Strain morphology identification: the screened Aspergillus terreus strain SYAT-1 (hereinafter referred to as: strain SYAT-1) was inoculated on PDA medium, cultured at 28°C for one week, the colony morphology was observed, and its sporulation structure and hyphae morphology were examined under a microscope. After culturing on a PDA plate at 28°C for 6 days, the bacteria formed white colonies, which were rou...

Embodiment 2

[0033] Example 2. Salt tolerance identification of bacterial strain SYAT-1

[0034] The PDA medium with gradient NaCl content was prepared, and its salt concentration was increased from 10% to 20% (saturation concentration), and each level increased by 2.5%, and PDA without addition was used as a control. An equal amount of strains were inoculated in the center of the culture medium, and cultured at 25°C and 25°C for 24 days. It was found that strain SYAT-1 could grow on 0%-17.5% NaCl PDA medium at 25°C and 28°C, but could not grow on 20% NaCl PDA medium. Wherein bacterial strain grows fastest on 0% NaCl PDA medium, along with salt concentration increases bacterial colony growth is slow (such as image 3 , Figure 4 Shown), so the strain belongs to the high salt-tolerant fungi.

Embodiment 3

[0035] Embodiment 3. bacterial strain SYAT-1 growth pH measurement

[0036] The growth time of strain SYAT-1 was different in different pH media. After 1 day of culture, colonies were grown at pH 7.0-10.0, and colonies at pH 11.0 and pH 12.0 were grown after 2 days. It can grow on the PDA medium with pH 7.0-12.0, indicating that the pH range of strain SYAT-1 is pH 7.0--12.0. The growth curves of strain SYAT-1 cultured in PDA medium with different pH for 8 days showed that the optimum pH range for its growth was 7.0-9.0.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
antibacterial rateaaaaaaaaaa
Login to View More

Abstract

The invention discloses an aspergillus terreus strain SYAT-1 resistant to saline-alkali and pathogenic fungi and an application thereof, and belongs to the technical field of microorganisms, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 9, 2020, the preservation name is aspergillus terreus SYAT-1, and the preservation number is CGMCC No.21021; the aspergillus terreus strain SYAT-1 disclosed by the invention has a salt-tolerant characteristic and can tolerate salt stress caused by sodium salt of 17.5% or below; the strain also has alkali-resistant characteristic and can tolerate alkali stress with pH below 12, and the optimal growth pH range of the strain is 7.0-9.0; and in addition, the strain has an inhibition effect on plant pathogenic fungi. The aspergillus terreus strain SYAT-1 disclosed by the invention has important application value in the aspects of improvement of saline-alkali soil and biological prevention and control of fungal diseases of crops.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a saline-alkali-resistant and anti-pathogenic fungus Aspergillus terreus strain SYAT-1 and its application. Background technique [0002] Soil salinization seriously restricts the sustainable development of agricultural production and resources and environment. my country is a big country with saline-alkali land, the area of ​​saline-alkali land ranks third in the world, and the existing saline-alkali land is 1.48 billion mu. Unreasonable human reclamation of wasteland, improper irrigation and agricultural measures, excessive use of pesticides and fertilizers, and facility soil cultivation will all lead to the formation of secondary salinization of the soil, and at the same time aggravate the occurrence of plant diseases and insect pests. Therefore, it has the function of biocontrol and can be used in The screening and research of microorganisms living in salin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/34A01P3/00C09K17/14C12R1/66C09K109/00
CPCA01N63/34C09K17/14C09K2109/00
Inventor 刘金亮王凤婷孙颖潘洪玉孙玥张祥辉
Owner JILIN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com