Macrolide composition for preventing and controlling fungal disease of crop and preparation process thereof

The technology of a composition and a fungicide is applied in the field of agricultural antibiotic preparation technology for preventing and controlling fungal diseases of crops, and can solve the problem that the diversity is far from being explored.

Inactive Publication Date: 2010-06-09
YUNNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the continuously increasing number of natural compounds shows t

Method used

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  • Macrolide composition for preventing and controlling fungal disease of crop and preparation process thereof
  • Macrolide composition for preventing and controlling fungal disease of crop and preparation process thereof
  • Macrolide composition for preventing and controlling fungal disease of crop and preparation process thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A strain that can produce fungicidal agricultural antibiotics, its scientific name is Streptomyces malaysiensis (Streptomyces malaysiensis) ECO 00002. This strain was deposited in the General Microbiology Center of the China Microbial Species Collection Management Committee on April 17, 2006, and was registered as The book number is CGMCC No.1680. The cell wall of the strain contains L,L-diaminopimelic acid, the whole cell sugar hydrolysate contains galactose and arabinose, and the cell wall is type I. The strain grows well on Gao's No. 1, glycerol-asparagine, yeast extract-malt extract, oatmeal, potato juice and nutrient agar medium; the morphological characteristics on Gao's No. 1 agar medium are: colony White, round, fluffy on the surface, with more hyphae growing on the edge; intrabasal hyphae and aerial hyphae are filamentous, irregularly branched, and generally not broken; long spore chains are formed on aerial hyphae, straight , Wave or spiral, spores are round, ...

Embodiment 2

[0062] The finished product of WYLZ-1349 prepared according to Example 1 was diluted to 50-200 mg / L, and tricyclazole (75% wettable powder), Sclerotium (40% wettable powder), prodyne (50% wettable powder) were used respectively. % Wettable powder) and chlorothalonil (75% wettable powder) as control agents, and clear water as a blank control to test the control effect of WYLZ-1349 products on rice blast, tobacco brown spot, tomato gray mold and pepper anthracnose The test results show that WYLZ-1349 can effectively control rice blast, tobacco brown spot, tomato gray mold and pepper anthracnose, and the dosage is significantly lower than the control agent. The test results are shown in Table 7 to Table 10.

[0063] Table 7 Test results of WYLZ-1349 controlling rice blast

[0064] Treatment agent

Use concentration (mg / L)

Average control effect (%)

WYLZ-1349

100

73.3

WYLZ-1349

200

81.9

WYLZ-1349

300

95.1

Tricyclazole

400

97.6

Water control

-

-

[0065] Table...

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Abstract

The invention provides an agricultural fungicide WYLZ-1349 taking azalomycin F3, azalomycin F4 and a nyphimycin composition as effective constituents, as well as preparation method and application thereof. The preparation method of the agricultural fungicide WYLZ-1349 comprises the following steps of: firstly culturing Streptomyces malaysiensis ECO 00002 with the preservation number of CGMCCNo. 1680; then separately extracting a fermentation culture; breaking the wall of mycelium with acetone or methanol and mixing with a fermentation supernate; and absorbing by macroporous absorption resin for separation; eluting by the acetone or the methanol; concentrating or further purifying an eluent so as to obtain the agricultural fungicide WYLZ-1349 or the azalomycin F3, the azalomycin F4 and nyphimycin; and then adding a carrier or an auxiliary agent so as to obtain a finished product. Test results show that the agricultural fungicide WYLZ-1349 has good effect especially in preventing and controlling rice blast, tobacco brown spot, tomato gray mold and pepper anthracnose and has small using amount, low using cost for peasants, wide usable range, and the like compared with the traditional medicament.

Description

Technical field [0001] The invention belongs to the technical field of pesticide production, and particularly relates to a preparation process and application of an agricultural antibiotic for preventing and treating crop fungal diseases. Background technique [0002] At present, human beings have stricter and stricter requirements on the environment, and the development of chemical pesticides is becoming more and more difficult and the success rate is getting lower and lower. After decades of screening, almost every imaginable chemical structure has been covered. For this reason, the world’s industrialized countries and major pesticide companies are looking for new development sites. At the same time, in order to find new lead compounds, the research and development of pesticides has entered an era of returning to nature, that is, looking for developable pesticides or pesticides from natural products. Lead compound. Since natural products have undergone long-term evolutionary ...

Claims

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Application Information

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IPC IPC(8): A01N43/90A01P3/00C12P17/18C07D493/08C12R1/465
Inventor 李铭刚杨佩文文孟良李家瑞赵江源尚慧
Owner YUNNAN UNIV
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