Device and method for detection and prevention of crop fungal diseases based on microfluidic chip

Abstract

The invention discloses a crop fungal disease prevention and detection device and method based on a microfluidic chip in the field of microbial detection. The output shaft of the stepper motor is vertically upward and fixedly connected to the center of the microfluidic chip. There are multiple devices on the microfluidic chip. Microfluidic detection channels with the same level. The pneumatic solenoid valve group is composed of multiple pneumatic solenoid valves with the same structure. One end of the air inlet of each pneumatic solenoid valve is connected to a chip air extraction port through an air extraction hose. The pneumatic solenoid valve The other end of the airway is connected to the micro air pump through the air outlet of the pneumatic solenoid valve; it adopts an array rotating microfluidic chip capture structure, which has functions such as spontaneous filtration, negative pressure suction, enrichment detection, and electric air pumping, and can be used in the field High-throughput detection of fungal diseases of the same crop on-site can also realize portable, fast and efficient capture of fungi of different crops, enabling early detection and warning of diseases.

Description

technical field [0001] The invention belongs to the field of microorganism detection, in particular to a detection device for one or more crop fungal spores based on an array type rotating microfluidic chip. Background technique [0002] Microbiological detection plays a decisive role in clinical diagnosis, food testing, industrial sewage treatment, preparation of agricultural nutrient solutions, etc., and microfluidic chips have developed rapidly in the field of microbial preventive detection. [0003] Chinese patent application number CN201410623883.1 discloses a plant disease intelligent diagnosis system, including a plant disease intelligent diagnosis host and a computer. The intelligent diagnosis host is equipped with a spectrum scanning device, and the intelligent diagnosis host is equipped with an importable spectrum scanning device. The detection tank, the intelligent diagnosis host is connected to the computer through the data line, it is powerful, and can realize t...

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Problems solved by technology

[0003] Chinese patent application number CN201410623883.1 discloses a plant disease intelligent diagnosis system, including a plant disease intelligent diagnosis host and a computer. The intelligent diagnosis host is equipped with a spectrum scanning device, and the intelligent diagnosis host is equipped with an importable spectrum scanning device. The detection tank, the intelligent diagnosis host is connected to the computer through the data line, it is powerful, and can realize the fast, accurate, stable, real-time and non-destructive diagnosis of various plant diseases, but the diagnosis system is used in plants with less fungal spores. At this time, the enrichment of fungal spores cannot be realized, so that real-time early disease detection cannot be realized, and there is hysteresis, and the diagnostic system has many problems such as large detection volume, high cost, inconvenient portability, and requires specialized personnel for detection, which is not conducive to promotion.

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