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A salinity-tolerant and anti-pathogenic fungus Aspergillus terreus strain syat-1 and its application

A SYAT-1, Aspergillus terreus technology, applied in the field of microorganisms, can solve the problem that Aspergillus terreus is not found to be resistant to plant pathogenic fungi, etc.

Active Publication Date: 2022-03-08
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the resistance of Aspergillus terreus to plant pathogenic fungi

Method used

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  • A salinity-tolerant and anti-pathogenic fungus Aspergillus terreus strain syat-1 and its application
  • A salinity-tolerant and anti-pathogenic fungus Aspergillus terreus strain syat-1 and its application
  • A salinity-tolerant and anti-pathogenic fungus Aspergillus terreus strain syat-1 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. Isolation and identification of Aspergillus terreus strain SYAT-1

[0029] Isolation of the strains: The soil samples for the isolation of the strains were from Songyuan City, Jilin Province. Select the land with high salinization, take the soil below 2cm of the soil surface, put it into a sterilized glass bottle and bring it back to the laboratory for later use. Using the dilution coating method, the diluted and clarified soil supernatant was spread on the PDA medium, and a fungal strain was obtained through screening, isolation and purification.

[0030] Strain morphology identification: the screened Aspergillus terreus strain SYAT-1 (hereinafter referred to as: strain SYAT-1) was inoculated on PDA medium, cultured at 28°C for one week, the colony morphology was observed, and its sporulation structure and hyphae morphology were examined under a microscope. After culturing on a PDA plate at 28°C for 6 days, the bacteria formed a white colony, which was roun...

Embodiment 2

[0033] Example 2. Salt tolerance identification of bacterial strain SYAT-1

[0034] The PDA medium with gradient NaCl content was prepared, and its salt concentration was increased from 10% to 20% (saturation concentration), and each level increased by 2.5%, and PDA without addition was used as a control. An equal amount of strains were inoculated in the center of the culture medium, and cultured at 25°C and 25°C for 24 days. It was found that strain SYAT-1 could grow on 0%-17.5% NaCl PDA medium at 25°C and 28°C, but could not grow on 20% NaCl PDA medium. Wherein bacterial strain grows fastest on 0% NaCl PDA medium, along with salt concentration increases bacterial colony growth is slow (such as image 3 , Figure 4 Shown), so the strain belongs to the high salt-tolerant fungi.

Embodiment 3

[0035] Embodiment 3. bacterial strain SYAT-1 growth pH measurement

[0036] The growth time of strain SYAT-1 was different in different pH media. After 1 day of culture, colonies were grown at pH 7.0-10.0, and colonies at pH 11.0 and pH 12.0 were grown after 2 days. It can grow on the PDA medium with pH 7.0-12.0, indicating that the pH range of strain SYAT-1 is pH 7.0--12.0. The growth curves of strain SYAT-1 cultured in PDA medium with different pH for 8 days showed that the optimum pH range for its growth was 7.0-9.0.

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Abstract

A salt-alkali-resistant and anti-pathogenic fungus Aspergillus terreus strain SYAT‑1 and its application belong to the field of microbial technology. The strain of the present invention has been preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on November 9, 2020. The preservation name It is Aspergillus terreus SYAT‑1, and the preservation number is CGMCC No.21021; the Aspergillus terreus strain SYAT‑1 of the present invention has salt tolerance, and it can tolerate salt stress caused by sodium salt below 17.5%; the strain also has alkali resistance , it can tolerate alkali stress below pH=12, and its optimum pH range for growth is 7.0-9.0; in addition, the bacterial strain has inhibitory effect on plant pathogenic fungi. The Aspergillus terreus strain SYAT-1 of the present invention has important application value in saline-alkali soil improvement and biological control of crop fungal diseases.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a saline-alkali-resistant and anti-pathogenic fungus Aspergillus terreus strain SYAT-1 and its application. Background technique [0002] Soil salinization seriously restricts the sustainable development of agricultural production and resources and environment. my country is a big country with saline-alkali land, the area of ​​saline-alkali land ranks third in the world, and the existing saline-alkali land is 1.48 billion mu. Unreasonable human reclamation of wasteland, improper irrigation and agricultural measures, excessive use of pesticides and fertilizers, and facility soil cultivation will all lead to the formation of secondary salinization of the soil, and at the same time aggravate the occurrence of plant diseases and insect pests. Therefore, it has the function of biocontrol and can be used in The screening and research of microorganisms living in salin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/34A01P3/00C09K17/14C12R1/66C09K109/00
CPCA01N63/34C09K17/14C09K2109/00
Inventor 刘金亮王凤婷孙颖潘洪玉孙玥张祥辉
Owner JILIN UNIV
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