Lithospermum extract and pesticide-containing synergistic composition for treating crop fungal diseases
A technology of comfrey extract and fungal diseases, applied in botany equipment and methods, chemicals for biological control, applications, etc., can solve the problem of no research reports on sclerotinia
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Embodiment 1
[0012] A crop fungal disease synergistic composition containing comfrey extract and pesticides, comprising a component A and a component B, the component A is a traditional Chinese medicine comfrey extract, and the component B is composed of prochloraz Medicine composition; wherein the A component is 50 grams, and the B component is 1 gram.
[0013] The preparation method of the composition of the crop fungal disease synergistic composition containing comfrey extract and pesticide includes the following steps: each component is weighed according to the above weight, and the component A is first dissolved in 1 volume Then use 8 times the volume of 1-2wt% NaOH to completely dissolve the absolute ethanol, and mix the extract with the B component to make a synergistic composition of traditional Chinese medicine and pesticides.
Embodiment 2
[0015] A crop fungal disease synergistic composition containing comfrey extract and pesticides, comprising a component A and a component B, the component A is a traditional Chinese medicine comfrey extract, and the component B is composed of prochloraz Medicine composition; wherein the A component is 200 grams, and the B component is 1 gram.
[0016] The crop fungal disease synergistic composition containing comfrey extract and pesticide comprises the following steps: each component is weighed according to the above-mentioned weight, and the A component Chinese medicine comfrey extract is 10:1, The A component is first dissolved in 1 volume of anhydrous ethanol, and then it is completely dissolved with 8 volumes of 1-2wt% NaOH, and the extract is mixed with the B component to prepare traditional Chinese medicines and pesticides The synergistic composition.
Embodiment 3
[0018] The composition in Example 1 or 2 was selected to detect its effect on Sclerotinia sclerotiorum ( Sclerotinia sclerotiorum ), Banana Fusarium Wilt ( Fusariun oxysporun ), Aspergillus flavus ( Aspergillus flavus ), Botrytis cinerea ( Botrytis cinerea ) And other crop pathogenic fungi growth rate inhibition. On the ultra-clean workbench, first inoculate Fujian isolated sclerotinia, banana fusarium wilt, aspergillus, and gray mold on the PDA solid medium, and cultivate in the dark for 48-72 hours at 25°C. On the ultra-clean workbench, inoculate the cultivated 5mm small plates of Sclerotinia sclerotiorum, Banana Fusarium wilt, Aspergillus flavus, and Botrytis cinerea into the PDA medium (containing the 1000-fold dilution of Example 1 or 2) The growth rate of pathogenic bacteria was calculated by the cross method after 48-72 hours of dark culture at a temperature of 25°C. The growth rate of the PDA medium without the composition was used as the control. The growth rate s...
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