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Plant pathogen inhibitor and strain involved therein and application of plant pathogen inhibitor

一种抑制剂、病原菌的技术，应用在植物生长调节剂、应用、杀菌剂等方向，能够解决病原菌易产生抗药性环境、污染、农药残留等问题，达到良好广谱拮抗活性、促进发芽、良好离体防效的效果

Active Publication Date: 2019-09-20
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prevention and control of fruit and vegetable gray mold in my country currently mainly relies on chemical agents, but the long-term use of chemical agents in large quantities has problems such as pesticide residues, pathogenic bacteria prone to drug resistance, and environmental pollution.

Method used

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  • Plant pathogen inhibitor and strain involved therein and application of plant pathogen inhibitor
  • Plant pathogen inhibitor and strain involved therein and application of plant pathogen inhibitor
  • Plant pathogen inhibitor and strain involved therein and application of plant pathogen inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Embodiment 1, the isolation and identification of Streptomyces alboniger (Streptomyces alboniger) T22

[0095] 1.1 Strain isolation

[0096]The strain was isolated from soil samples in special habitats of Qinghai-Tibet Plateau, Qinghai Province, China. The bacterial strains were separated by the dilution plate method, and the specific operation method was as follows: Weighed 10 g of soil samples, poured them into a conical flask filled with small glass beads and 90 mL of sterile water, oscillated for 30 min, then allowed to stand for 5 min, diluted 10 times in turn, and prepared respectively 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Suspension of different concentrations, draw 0.1mL of suspension of different concentrations and add to Gao's No. 1 medium (adding K with a final concentration of 75mg / L 2 CrO 7 ) plate, spread evenly and place it at 28°C for observation. After 5-7 days, different single colonies were picked and streaked for purification. The purified strain...

Embodiment 2

[0121] The antibacterial activity of embodiment 2, streptomyces alboniger (Streptomyces alboniger) T22

[0122] 2.1 Agar block method: use a sterile pipette to absorb 5 mL of sterile water on the slant of the target pathogenic bacteria culture to be tested, scrape off the hyphae with a bamboo stick to prepare a suspension of pathogenic bacteria, and use a 1 mL sterile pipette to absorb 0.1 mL of the bacterial suspension Spread evenly on PDA (pathogenic fungi) or LB (pathogenic bacteria) plates.

[0123] The pathogenic fungi tested were Botrytis cinerea, Botrytis cinerea, Botrytis cinerea, Monilinia fructicola, Colletotrichum capsici, crosslinked Alternaria alternata, Fusarium oxysporum f.sp.cucumerinum, Fusarium oxysporum f.sp.vasinfectum, Fusarium oxysporum f.sp.conglutinans, Wheat sheath blight Rhizoctonia cereali, Fusarium moniliforme.

[0124] The pathogenic bacteria tested were Ralstonia solanacearum, Xanthomonas campestris pv. campsetris, and Pseudomonas syringaepv. la...

Embodiment 3

[0135] Embodiment 3, Streptomyces alboniger (Streptomyces alboniger) T22 produces cellulase and chitinase activity

[0136] Pick the Streptomyces alboniger T22 grown on the plate with the inoculation loop in the cellulase-producing medium and chitinase-producing medium, and culture it at 28°C for 7 days, then add 2g / L Congo red staining solution to the plate 5mL, rinsed with deionized water after staining for 30min, then decolorized with 1M NaCl solution, rinsed with deionized water, and observed the formation of hydrolysis circles around the colonies.

[0137] Cellulase producing medium: cellulose powder 5.0g, (NH 4 ) 2 SO 4 2.0g, KH 2 PO 4 1.0g,

[0138] NaCl 0.5g, MgSO 4 ·7H 2 O 0.5g, agar 18.0g, distilled water 1000

[0139] mL.

[0140] Chitinase-producing medium: colloidal chitin 2.5g, K 2 HPO 4 0.7g, K 2 HPO 4 0.3g, MgSO 4 ·7H 2 O0.5g, FeSO 4 ·7H 2 O 0.01g, agar 18.0g, distilled water 1000mL.

[0141] The results showed that a hydrolysis circle for...

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Abstract

The invention discloses a plant pathogen inhibitor and a strain involved therein and an application of the plant pathogen inhibitor. The strain is streptoverticillium davidii T22 which is registered in the General Microbiology Center of the China microbial species Preservation and Management Committee with the number of CGMCC No. 14190. The streptoverticillium davidii T22 has inhibitory effect on botrytis cinerea, peach brown rot and capsicum anthracnose in fruits and vegetables, streptoverticillium davidii T22 aseptic fermentation filtrate can effectively inhibit the hyphal growth of strawberry gray mold, tomato gray mold and cucumber fusarium wilt, and the streptoverticillium davidii can form hydrolysis cycles on the medium for enzyme activity detection by producing cellulase and chitinase. Obviously, the streptoverticillium davidii T22 can inhibit plant pathogenic fungi by producing antibiotic-like active substances and hydrolase-like substances; proper dilution of the streptoverticillium davidii T22 aseptic fermentation filtrate can significantly promote the extension of tomato hypocotyl and / or radicle and enhance the germination potential of tomato seeds.

Description

[0001] This application is a divisional application with the application number 201710506536.4, the application date is June 28, 2017, and the invention title is "Preparation and Application of a Strain of Streptomyces Alboblack and Its Metabolites for Disease Prevention and Growth Promotion". technical field [0002] The invention belongs to the technical field of microbial pesticides, and in particular relates to an inhibitor of plant pathogenic bacteria as well as the used strain and application thereof. Background technique [0003] Botrytis cinera, caused by the fungus Botrytis cinera, is common and serious in many fruit and vegetable crops. The disease can harm the stems, leaves, flowers and fruits of plants, especially in humid and rainy and greenhouse environments. The prevention and control of fruit and vegetable gray mold in my country currently mainly relies on chemical agents, but the long-term use of chemical agents in large quantities has problems such as pesti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/465
CPCA01N63/00C12N1/20C12R2001/465C12N1/205A01N63/10
Inventor 赵娟刘伟成张殿朋刘霆刘德文吴慧玲卢彩鸽董丹
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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