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Preparation and application of a strain of Streptomyces alboniger for disease prevention and growth promotion and its metabolites

A technology of Streptomyces alboblack and Streptomyces niger, applied in the direction of microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the problems of pollution, pathogenic bacteria prone to drug resistance, pesticide residues, etc., to promote germination, good propagation Spectrum antagonistic activity, good in vitro control effect

Active Publication Date: 2019-07-26
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The prevention and control of fruit and vegetable gray mold in my country currently mainly relies on chemical agents, but the long-term use of chemical agents in large quantities has problems such as pesticide residues, pathogenic bacteria prone to drug resistance, and environmental pollution.

Method used

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  • Preparation and application of a strain of Streptomyces alboniger for disease prevention and growth promotion and its metabolites
  • Preparation and application of a strain of Streptomyces alboniger for disease prevention and growth promotion and its metabolites
  • Preparation and application of a strain of Streptomyces alboniger for disease prevention and growth promotion and its metabolites

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Embodiment 1, the isolation and identification of Streptomyces alboniger (Streptomyces alboniger) T22

[0094] 1.1 Strain isolation

[0095] The strain was isolated from soil samples in special habitats of Qinghai-Tibet Plateau, Qinghai Province, China. The bacterial strains were separated by the dilution plate method, and the specific operation method was as follows: Weighed 10 g of soil samples, poured them into a conical flask filled with small glass beads and 90 mL of sterile water, oscillated for 30 min, then allowed to stand for 5 min, diluted 10 times sequentially, and prepared respectively 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Suspension of different concentrations, draw 0.1mL of suspension of different concentrations and add to Gao's No. 1 medium (adding K with a final concentration of 75mg / L 2 CrO 7 ) plate, spread evenly and place it at 28°C for observation. After 5-7 days, different single colonies were picked and streaked for purification. The purified ...

Embodiment 2

[0118] The antibacterial activity of embodiment 2, streptomyces alboniger (Streptomyces alboniger) T22

[0119] 2.1 Agar block method: use a sterile pipette to absorb 5 mL of sterile water on the slant of the target pathogenic bacteria culture to be tested, scrape off the hyphae with a bamboo stick to prepare a suspension of pathogenic bacteria, and use a 1 mL sterile pipette to absorb 0.1 mL of the bacterial suspension Spread evenly on PDA (pathogenic fungi) or LB (pathogenic bacteria) plates.

[0120] The pathogenic fungi tested were Botrytis cinerea, Botrytis cinerea, Botrytis cinerea, Monilinia fructicola, Colletotrichum capsici, crosslinked Alternaria alternata, Fusarium oxysporum f.sp.cucumerinum, Fusarium oxysporum f.sp.vasinfectum, Fusarium oxysporum f.sp.conglutinans, Wheat sheath blight Rhizoctonia cereali, Fusarium moniliforme.

[0121] The pathogenic bacteria tested were Ralstonia solanacearum, Xanthomonas campestris pv. campsetris, and Pseudomonas syringaepv. la...

Embodiment 3

[0132] Embodiment 3, Streptomyces alboniger (Streptomyces alboniger) T22 produces cellulase and chitinase activity

[0133] Pick the Streptomyces alboniger T22 grown on the plate with the inoculation loop in the cellulase-producing medium and chitinase-producing medium, and culture it at 28°C for 7 days, then add 2g / L Congo red staining solution to the plate 5mL, rinsed with deionized water after staining for 30min, then decolorized with 1M NaCl solution, rinsed with deionized water, and observed the formation of hydrolysis circles around the colonies.

[0134] Cellulase producing medium: cellulose powder 5.0g, (NH 4 ) 2 SO 4 2.0g, KH 2 PO 4 1.0g, NaCl 0.5g, MgSO 4 ·7H 2 O 0.5g, agar 18.0g, distilled water 1000mL.

[0135] Chitinase-producing medium: colloidal chitin 2.5g, K 2 HPO 4 0.7g, K 2 HPO 4 0.3g, MgSO 4 ·7H 2 O0.5g, FeSO 4 ·7H 2 O 0.01g, agar 18.0g, distilled water 1000mL.

[0136] The results showed that a hydrolysis circle formed around Streptomyc...

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Abstract

The invention discloses the preparation and application of a strain of Streptomyces albigeris for disease prevention and growth promotion and its metabolites. The strain number of the Streptomyces albonigeris is T22, and its registration number in the General Microorganism Center of China Committee for Culture Collection of Microorganisms is CGMCC No.14190. Streptomyces alboblack T22 has inhibitory effects on botrytis cinerea, peach brown rot and capsicum anthracnose in fruit and vegetable crops. The aseptic fermentation filtrate of Streptomyces alboblack T22 can effectively inhibit the mycelial growth of Botrytis cinerea, Botrytis cinerea and Fusarium wilt of cucumber. Streptomyces alboniger can form a hydrolytic circle on the enzyme production assay medium by producing cellulase and chitinase. It shows that Streptomyces alboniger T22 can inhibit plant pathogenic fungi by producing antibiotic active substances and hydrolytic enzyme substances. Appropriate dilution of the aseptic fermentation filtrate of Streptomyces albus T22 can significantly promote the elongation of tomato hypocotyls and / or radicles, and enhance the germination potential of tomato seeds.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to the preparation and application of a Streptomyces albus and its metabolites for disease prevention and growth promotion. Background technique [0002] Botrytis cinera, caused by the fungus Botrytis cinera, is common and serious in many fruit and vegetable crops. The disease can harm the stems, leaves, flowers and fruits of plants, especially in humid and rainy and greenhouse environments. The prevention and control of fruit and vegetable gray mold in my country currently mainly relies on chemical agents, but the long-term use of chemical agents in large quantities has problems such as pesticide residues, pathogenic bacteria prone to drug resistance, and environmental pollution. Exploring new high-efficiency, broad-spectrum, low-toxicity, and environmental-friendly plant disease control technologies is a difficult and hot spot in agricultural production r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04A01N63/00A01N63/02A01P3/00A01P21/00C12R1/465
CPCA01N63/00C12N1/20C12R2001/465C12N1/205A01N63/10
Inventor 赵娟刘伟成张殿朋刘霆刘德文吴慧玲卢彩鸽董丹
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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