Composite microbiological bacterial agent used for returning maize straws to field and preparation method and applications thereof
A technology of compound microbial bacterial agent and corn stalks, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, chemicals for biological control, etc., can solve the problems of accelerating the return of corn stalks to the field, which has not yet been discovered, and achieves good results. Synergistic effect, accelerated decomposition speed, and accelerated release of nutrient elements
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Embodiment 1
[0039] The composition of embodiment 1 composite microbial bacterial agent
[0040] Arthrobacter 1.0×10 10 cfu / g
[0041] Bacillus subtilis 5.0×10 8 cfu / g
[0042] Phanerochaete chrysosporium 2.0×10 6 cfu / g
[0043] Trichoderma konshii 1.0×10 6 cfu / g
[0044] Aspergillus niger 1.0×10 4 cfu / g
[0045] Saccharomyces cerevisiae 1.0×10 8 cfu / g
[0046] Streptomyces albicans 2.0×10 8 cfu / g
[0047] Streptomyces hygroscopicus 2.0×10 6 cfu / g
[0048] The bacterial agent with the above constitutional method is prepared by the following steps.
[0049] 1) Incline culture: the original strains of Arthrobacter, Bacillus subtilis, Phanerochaete chrysosporium, Trichoderma konshii, Aspergillus niger, Saccharomyces cerevisiae, Streptomyces albicans, and Streptomyces hygroscopicus were respectively cultured under aseptic conditions. Inoculate on solid medium, culture Arthrobacter and Bacillus subtilis at 15°C for 2 days, Phanerochaete chrysosporium, Trichoderma konii, Aspergill...
Embodiment 2
[0056] The composition of embodiment 2 composite microbial bacterial agents
[0057] Arthrobacter 5.0×10 8 cfu / g
[0058] Bacillus subtilis 1.0×10 10 cfu / g
[0059] Phanerochaete chrysosporium 1.0×10 4 cfu / g
[0060] Trichoderma konshii 2.0×10 8 cfu / g
[0061] Aspergillus niger 2.0×10 6 cfu / g
[0062] Saccharomyces cerevisiae 2.0×10 6 cfu / g
[0063] Streptomyces albicans 2.0×10 6 cfu / g
[0064] Streptomyces hygroscopicus 2.0×10 8 cfu / g
[0065] The bacterial agent with the above constitutional method is prepared by the following steps.
[0066] 1) Incline culture: the original strains of Arthrobacter, Bacillus subtilis, Phanerochaete chrysosporium, Trichoderma konshii, Aspergillus niger, Saccharomyces cerevisiae, Streptomyces albicans, and Streptomyces hygroscopicus were respectively cultured under aseptic conditions. Inoculate on solid medium, culture Arthrobacter and Bacillus subtilis at 40°C for 3 days, Phanerochaete chrysosporium, Trichoderma konii, Aspergil...
Embodiment 3
[0073] Embodiment 3 Composition of composite microbial bacterial agent.
[0074] Arthrobacter 3.0×10 9 cfu / g
[0075] Bacillus subtilis 6.0×10 9 cfu / g
[0076] Phanerochaete chrysosporium 6.0×10 5 cfu / g
[0077] Trichoderma konshii 8.0×10 7 cfu / g
[0078] Aspergillus niger 6.0×10 5 cfu / g
[0079] Saccharomyces cerevisiae 5.0×10 7 cfu / g
[0080] Streptomyces albicans 8.0×10 7 cfu / g
[0081] Streptomyces hygroscopicus 1.0×10 8 cfu / g
[0082] The bacterial agent of above-mentioned composition is made by following steps
[0083] 1) Incline culture: Arthrobacter, Bacillus subtilis, Phanerochaete chrysosporium, Trichoderma konshii, Aspergillus niger, Saccharomyces cerevisiae, Streptomyces albicans, Streptomyces hygroscopicus, and the original strains were separated under sterile conditions. Inoculate on solid medium, culture Arthrobacter and Bacillus subtilis at 37°C for 3 days, Phanerochaete chrysosporium, Trichoderma konii, Aspergillus niger, Saccharomyces cerevisia...
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