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Composite microbiological bacterial agent used for returning maize straws to field and preparation method and applications thereof

A technology of composite microbial inoculants and corn stalks, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, and chemicals for biological control, etc. Decomposition speed, good synergistic effect, high active effect

Active Publication Date: 2011-09-07
北京沃土天地生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there has been no report on the application of compound microbial agents with disease-resistant effects to accelerate the return of corn stalks to the field under low temperature conditions.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The composition of embodiment 1 composite microbial bacterial agent

[0040] Arthrobacter 1.0×10 10 cfu / g

[0041] Bacillus subtilis 5.0×10 8 cfu / g

[0042] Phanerochaete chrysosporium 2.0×10 6 cfu / g

[0043] Trichoderma konshii 1.0×10 6 cfu / g

[0044] Aspergillus niger 1.0×10 4 cfu / g

[0045] Saccharomyces cerevisiae 1.0×10 8 cfu / g

[0046] Streptomyces albicans 2.0×10 8 cfu / g

[0047] Streptomyces hygroscopicus 2.0×10 6 cfu / g

[0048] The bacterial agent with the above constitutional method is prepared by the following steps.

[0049] 1) Incline culture: the original strains of Arthrobacter, Bacillus subtilis, Phanerochaete chrysosporium, Trichoderma konshii, Aspergillus niger, Saccharomyces cerevisiae, Streptomyces albicans, and Streptomyces hygroscopicus were respectively cultured under aseptic conditions. Inoculate on solid medium, culture Arthrobacter and Bacillus subtilis at 15°C for 2 days, Phanerochaete chrysosporium, Trichoderma konii, Aspergill...

Embodiment 2

[0056] The composition of embodiment 2 composite microbial bacterial agents

[0057] Arthrobacter 5.0×10 8 cfu / g

[0058] Bacillus subtilis 1.0×10 10 cfu / g

[0059] Phanerochaete chrysosporium 1.0×10 4 cfu / g

[0060] Trichoderma konshii 2.0×10 8 cfu / g

[0061] Aspergillus niger 2.0×10 6 cfu / g

[0062] Saccharomyces cerevisiae 2.0×10 6 cfu / g

[0063] Streptomyces albicans 2.0×10 6 cfu / g

[0064] Streptomyces hygroscopicus 2.0×10 8 cfu / g

[0065] The bacterial agent with the above constitutional method is prepared by the following steps.

[0066] 1) Incline culture: the original strains of Arthrobacter, Bacillus subtilis, Phanerochaete chrysosporium, Trichoderma konshii, Aspergillus niger, Saccharomyces cerevisiae, Streptomyces albicans, and Streptomyces hygroscopicus were respectively cultured under aseptic conditions. Inoculate on solid medium, culture Arthrobacter and Bacillus subtilis at 40°C for 3 days, Phanerochaete chrysosporium, Trichoderma konii, Aspergil...

Embodiment 3

[0073] Embodiment 3 Composition of composite microbial bacterial agent.

[0074] Arthrobacter 3.0×10 9 cfu / g

[0075] Bacillus subtilis 6.0×10 9 cfu / g

[0076] Phanerochaete chrysosporium 6.0×10 5 cfu / g

[0077] Trichoderma konshii 8.0×10 7 cfu / g

[0078] Aspergillus niger 6.0×105 cfu / g

[0079] Saccharomyces cerevisiae 5.0×10 7 cfu / g

[0080] Streptomyces albicans 8.0×10 7 cfu / g

[0081] Streptomyces hygroscopicus 1.0×10 8 cfu / g

[0082] The bacterial agent of above-mentioned composition is made by following steps

[0083] 1) Incline culture: Arthrobacter, Bacillus subtilis, Phanerochaete chrysosporium, Trichoderma konshii, Aspergillus niger, Saccharomyces cerevisiae, Streptomyces albicans, Streptomyces hygroscopicus, and the original strains were separated under sterile conditions. Inoculate on solid medium, culture Arthrobacter and Bacillus subtilis at 37°C for 3 days, Phanerochaete chrysosporium, Trichoderma konii, Aspergillus niger, Saccharomyces cerevisiae ...

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Abstract

The invention discloses a composite microbiological bacterial agent used for returning maize straws to a field and a preparation method and applications thereof. The composite microbiological bacterial agent comprises Arthrobactersp, Bacillus subtilis, Phanerochaetechrysosporium, Trichoderma koningii, Aspergillusniger, Saccharomyces cerevisiae, Streptomyces alboniger, and Streptomyces hygroscopicus. The preparation method comprises the following steps: carrying out slant culture, fermenting one-grade seeds and second-grade seeds and mixing, culturing, and fermenting the one-grade seeds and the second-grade seeds, realizing the optimization of compound bacterial biomass and the matching of different strains, and the compound bacterium effective viable count can reach 1010cfu per gram. By utilizing the compound microbiological bacterial agent, the decomposition velocity of organic matters can be accelerated, the straw can be decomposed and blackened within 10 days at the low temperature of 10-15 DEG C; the straw is basically well-rotted; and the composite microbiological bacterial agent has certain disease-resisting effect, and provides necessary condition for preventing the diseases of wheat roots.

Description

technical field [0001] The invention relates to a composite microbial bacterial agent, in particular to a composite microbial bacterial agent which uses microorganisms to treat corn stalks to accelerate the rapid return of the straw to the field. The present invention also relates to the preparation method and application of the composite microbial bacterial agent. Background technique [0002] Crop straw is one of the most abundant substances in the world. At present, the solution to the problem of large areas of straw in my country mainly relies on direct return to the field. Especially in the north, under the double cropping system of corn and wheat, returning a large amount of corn stalks to the field year after year has shown many benefits to the soil ecosystem of wheat fields. It is not only conducive to the accumulation of soil organic matter, but also reduces soil bulk density, increases soil porosity, improves soil permeability, and improves poorly infiltrated barr...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/14C12N1/18C12N1/20C05F11/08A01N63/00A01P1/00A01P3/00C12R1/06C12R1/125C12R1/645C12R1/885C12R1/685C12R1/865C12R1/465C12R1/55
Inventor 任莉李季彭生平
Owner 北京沃土天地生物科技股份有限公司
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