Composite microbiological bacterial agent used for returning maize straws to field and preparation method and applications thereof
A technology of composite microbial inoculants and corn stalks, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, and chemicals for biological control, etc. Decomposition speed, good synergistic effect, high active effect
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[0039] Example 1 Composition of composite microbial agent
[0040] Arthrobacter 1.0×10 10 cfu / g
[0041] Bacillus subtilis 5.0×10 8 cfu / g
[0042] Phanerochaete chrysosporium 2.0×10 6 cfu / g
[0043] Trichoderma konsi 1.0×10 6 cfu / g
[0044] Aspergillus niger 1.0×10 4 cfu / g
[0045] Saccharomyces cerevisiae 1.0×10 8 cfu / g
[0046] Streptomyces griseus 2.0×10 8 cfu / g
[0047] Streptomyces hygroscopicus 2.0×10 6 cfu / g
[0048] The inoculum with the above structure is prepared by the following steps.
[0049] 1) Slant culture: the original strains of Arthrobacter, Bacillus subtilis, Phanerochaete chrysosporium, Trichoderma konsi, Aspergillus niger, Saccharomyces cerevisiae, Streptomyces albicans, Streptomyces hygroscopicus, respectively Inoculate on a solid medium, culture Arthrobacter and Bacillus subtilis at 15℃ for 2 days, culture Phanerochaete chrysosporium, Trichoderma konsi, Aspergillus niger, and Saccharomyces cerevisiae for 2 days at 15℃. Streptomyces griseus cultured at 15℃ for 3 days...
Example Embodiment
[0056] Example 2 Composition of composite microbial agent
[0057] Arthrobacter 5.0×10 8 cfu / g
[0058] Bacillus subtilis 1.0×10 10 cfu / g
[0059] Phanerochaete chrysosporium 1.0×10 4 cfu / g
[0060] Trichoderma Koningii 2.0×10 8 cfu / g
[0061] Aspergillus niger 2.0×10 6 cfu / g
[0062] Saccharomyces cerevisiae 2.0×10 6 cfu / g
[0063] Streptomyces griseus 2.0×10 6 cfu / g
[0064] Streptomyces hygroscopicus 2.0×10 8 cfu / g
[0065] The inoculum with the above structure is prepared by the following steps.
[0066] 1) Slant culture: the original strains of Arthrobacter, Bacillus subtilis, Phanerochaete chrysosporium, Trichoderma konsi, Aspergillus niger, Saccharomyces cerevisiae, Streptomyces albicans, Streptomyces hygroscopicus, respectively, under aseptic conditions Inoculate on a solid medium, culture Arthrobacter and Bacillus subtilis at 40℃ for 3 days, culture Phanerochaete chrysosporium, Trichoderma konsi, Aspergillus niger, Saccharomyces cerevisiae for 4 days at 38℃, white light Cultivatio...
Example Embodiment
[0073] Example 3 The composition of the composite microbial agent.
[0074] Arthrobacter 3.0×10 9 cfu / g
[0075] Bacillus subtilis 6.0×10 9 cfu / g
[0076] Phanerochaete chrysosporium 6.0×10 5 cfu / g
[0077] Trichoderma konsi 8.0×10 7 cfu / g
[0078] Aspergillus niger 6.0×10 5 cfu / g
[0079] Saccharomyces cerevisiae 5.0×10 7 cfu / g
[0080] Streptomyces griseus 8.0×10 7 cfu / g
[0081] Streptomyces hygroscopicus 1.0×10 8 cfu / g
[0082] The bacterial agent of the above composition is prepared by the following steps
[0083] 1) Slant culture: the original strains of Arthrobacter, Bacillus subtilis, Phanerochaete chrysosporium, Trichoderma konsi, Aspergillus niger, Saccharomyces cerevisiae, Streptomyces albicans, Streptomyces hygroscopicus, and the original strains are separately under aseptic conditions Inoculate on a solid medium, culture Arthrobacter and Bacillus subtilis at 37℃ for 3 days, culture Phanerochaete chrysosporium, Trichoderma konsi, Aspergillus niger, Saccharomyces cerevisiae for 3...
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