Application of extract of celery stems and leaves
A technology of extracts and stems and leaves, applied in the field of celery stems and leaves extracts, can solve the problems of crop failure, tomato planting reduction, unfavorable health, etc., and achieve the effect of easy use, low cost, no residue and drug resistance problems
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Embodiment 1
[0016] Embodiment 1: The inhibitory action of celery stem and leaf extract to tomato solanacearum (Pseudomonas solanacearum)
[0017] 1. Collect stem and leaf extracts by grinding and ultra-low temperature evaporation. The specific steps are as follows:
[0018] 1) The variety of celery is 'Jinnan Shiqin No. 1', using nutrient bowls (peat: vermiculite = 3:1), 20 seeds are evenly sown in each bowl, cultivated in an artificial climate room (12 hours of artificial light, day and night temperature 25°C / 20°C); 2) After 60 days, collect the aboveground stems and leaves of cultivated celery (about 40-50cm high) with scissors, wash and chop, grind with a grinding rod, collect the filtrate after filtering through filter paper, and store it at -50°C Freeze and evaporate, concentrate to 1 / 5 of the original volume, that is, the concentrated solution of celery stem and leaf extract, filter and sterilize with a 0.22 micron filter head, and store at a low temperature of 4°C.
[0019] 2. Ps...
Embodiment 2
[0026] Example 2: Inhibitory effect of celery stem and leaf extract on tomato root rot
[0027] 1. The preparation of celery stem and leaf extract is as follows: after washing and chopping the stem and leaf of celery (Jinnan Shiqin No. 1), put it into a juice extractor to squeeze the juice, the obtained juice is coarsely filtered through filter paper, and the obtained filtrate is vacuum decompressed Concentrate to 20% of the original volume, and finally pass through a 0.22 micron filter head to filter and sterilize, and store at a low temperature of 4°C.
[0028] 2. The tomato root rot pathogen Fusarium oxysporum f.sp.Lycopersici (race 1) (provided by Zhejiang Academy of Agricultural Sciences) was used as the evaluation strain. The propagation conditions of this strain are as follows:
[0029] PDA medium preparation: 200g of fresh potatoes, add distilled water to boil, continue heating for 30 minutes, filter with 4 layers of gauze, add 18g of glucose to the filtrate, 20g of a...
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