Microorganism plant vaccine capable of controlling tomato bacterial wilt disease

Abstract

The invention relates to microorganism plant vaccine capable of controlling continuous tomato bacterial wilt disease, and belongs to the agricultural intensive production technology. According to the microorganism plant vaccine capable of controlling the tomato bacterial wilt disease, a ralstonia solanacearum sibling bacterium pearson ralstonia eutropha (Ralstoniapickettii) NJQL-A6 is isolated from the rhizosphere of a healthy tomato, and the ralstonia eutropha has a weaking inhibited impact on a bacterial wilt pathogenic bacteria ralstonia eutropha (Ralstonia solanacearum) panel. However, the ralstonia eutropha possesses powerful colonization capability on the tomato rhizosphere and inside a plant, and can inhibit the infection of the ralstonia eutropha to a tomato plant. Experiments show that after the vaccine is irrigated to be applied to soil, the vaccine can breed rapidly so that an advantage group is formed in the soil, and the biocontrol efficiency can be reached up to more than 70% in the soil where the tomato bacterial wilt disease is severe. By inoculating the biocontrol bateria on the tomato plant with the root being infected by the bacterial wilt on the overground part, the further development of the disease can be effectively controlled.

Description

1. Technical field

[0001] The invention relates to a microbial plant vaccine capable of preventing and eliminating bacterial wilt of continuous cropping tomato, which belongs to the intensive agricultural production technology and is specially used for overcoming and eliminating the problem of bacterial wilt of continuous cropping tomato. 2. Background technology

[0002] Tomato bacterial wilt is a bacterial soil-borne disease of vascular bundles caused by Ralstonia solanacearum. In 1906, American Simth scholars discovered and reported this disease for the first time. Up to now, R. solanacearum exists in other countries and regions except Antarctica. Ralstonia is divided into five physiological races and six biochemical races. All the races have certain pathogenicity to tomato, and race 1 has the strongest pathogenicity to tomato. In my country, tomato bacterial wilt mainly occurs in the warm and humid south. With the warming of the climate and the development of high-effi...

Examples

Embodiment Construction

[0021] (1) Isolation and identification of strains

[0022] The plant vaccine NJQL-A6 for preventing continuous cropping tomato bacterial wilt is isolated from the rhizosphere soil of healthy tomato plants in fields where continuous cropping tomato bacterial wilt occurs. By collecting continuous cropping obstacles and tomato bacterial wilt disease plants and the surrounding soil, the samples were stored at low temperature, and the M-SMSA bacterial wilt selective medium was used to obtain pathogenic strains and a large number of test biocontrol bacteria (suspected non-pathogenic) solanacearum), use CPG, TZC and M-SMSA medium to pass the plate confrontation test for primary screening, rhizosphere and stem base colonization ability test for re-screening, and finally determine the highly effective biocontrol strains through pot experiments, identify genus, species, and bacteria Freeze-dried and stored at -20°C.

[0023] The strain NJQL-A6 belongs to Ralstonia pickettii (Ralstonia...

1. Technical field

[0001] The invention relates to a microbial plant vaccine capable of preventing and eliminating bacterial wilt of continuous cropping tomato, which belongs to the intensive agricultural production technology and is specially used for overcoming and eliminating the problem of bacterial wilt of continuous cropping tomato. 2. Background technology

[0002] Tomato bacterial wilt is a bacterial soil-borne disease of vascular bundles caused by Ralstonia solanacearum. In 1906, American Simth scholars discovered and reported this disease for the first time. Up to now, R. solanacearum exists in other countries and regions except Antarctica. Ralstonia is divided into five physiological races and six biochemical races. All the races have certain pathogenicity to tomato, and race 1 has the strongest pathogenicity to tomato. In my country, tomato bacterial wilt mainly occurs in the warm and humid south. With the warming of the climate and the development of high-effi...

Examples

Embodiment Construction

[0021] (1) Isolation and identification of strains

[0022] The plant vaccine NJQL-A6 for preventing continuous cropping tomato bacterial wilt is isolated from the rhizosphere soil of healthy tomato plants in fields where continuous cropping tomato bacterial wilt occurs. By collecting continuous cropping obstacles and tomato bacterial wilt disease plants and the surrounding soil, the samples were stored at low temperature, and the M-SMSA bacterial wilt selective medium was used to obtain pathogenic strains and a large number of test biocontrol bacteria (suspected non-pathogenic) solanacearum), use CPG, TZC and M-SMSA medium to pass the plate confrontation test for primary screening, rhizosphere and stem base colonization ability test for re-screening, and finally determine the highly effective biocontrol strains through pot experiments, identify genus, species, and bacteria Freeze-dried and stored at -20°C.

[0023] The strain NJQL-A6 belongs to Ralstonia pickettii (Ralstonia...

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