Microorganism plant vaccine capable of controlling tomato bacterial wilt disease
A tomato bacterial wilt and plant vaccine technology, applied in the field of microbial plant vaccines, can solve the problems that there is no effective means to prevent and control tomato bacterial wilt, and the development of the tomato market has a great impact, and achieve less investment in fixed assets, low cost, and high production technology. easy effect
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[0021] (1) Isolation and identification of strains
[0022] The plant vaccine NJQL-A6 for the control of continuous cropping tomato bacterial wilt disease was isolated from the rhizosphere soil of healthy tomato plants in the field with continuous cropping tomato bacterial wilt disease. By collecting the plants and surrounding soils that have had continuous cropping obstacles and tomato bacterial wilt symptoms, the samples were stored at low temperature, and the M-SMSA bacterial wilt selective medium was used to obtain pathogenic strains and a large number of tested biocontrol bacteria (suspected to be non-pathogenic Ralstonia solanacearum), using CPG, TZC and M-SMSA medium respectively through the initial screening of the plate confrontation experiment, the rhizosphere and stem base colonization ability experiment re-screening, and finally through the pot experiment to determine the efficient biocontrol strains, identify the genus species, bacteria The seeds were lyophilized ...
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