Microorganism plant vaccine capable of controlling tomato bacterial wilt disease

A tomato bacterial wilt and plant vaccine technology, applied in the field of microbial plant vaccines, can solve the problems that there is no effective means to prevent and control tomato bacterial wilt, and the development of the tomato market has a great impact, and achieve less investment in fixed assets, low cost, and high production technology. easy effect

Active Publication Date: 2015-04-15
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

At present, many vegetable bases in the south are no longer suitable for growing tomatoes, which has a great impact on the development of my country's tomato market.
At present, there is no effective means to control tomato bacterial wilt, and the use of rhizosphere microorganisms to control diseases is one of the important measures for comprehensive control of soil-borne diseases

Method used

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  • Microorganism plant vaccine capable of controlling tomato bacterial wilt disease
  • Microorganism plant vaccine capable of controlling tomato bacterial wilt disease
  • Microorganism plant vaccine capable of controlling tomato bacterial wilt disease

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Embodiment Construction

[0021] (1) Isolation and identification of strains

[0022] The plant vaccine NJQL-A6 for the control of continuous cropping tomato bacterial wilt disease was isolated from the rhizosphere soil of healthy tomato plants in the field with continuous cropping tomato bacterial wilt disease. By collecting the plants and surrounding soils that have had continuous cropping obstacles and tomato bacterial wilt symptoms, the samples were stored at low temperature, and the M-SMSA bacterial wilt selective medium was used to obtain pathogenic strains and a large number of tested biocontrol bacteria (suspected to be non-pathogenic Ralstonia solanacearum), using CPG, TZC and M-SMSA medium respectively through the initial screening of the plate confrontation experiment, the rhizosphere and stem base colonization ability experiment re-screening, and finally through the pot experiment to determine the efficient biocontrol strains, identify the genus species, bacteria The seeds were lyophilized ...

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Abstract

The invention relates to microorganism plant vaccine capable of controlling continuous tomato bacterial wilt disease, and belongs to the agricultural intensive production technology. According to the microorganism plant vaccine capable of controlling the tomato bacterial wilt disease, a ralstonia solanacearum sibling bacterium pearson ralstonia eutropha (Ralstoniapickettii) NJQL-A6 is isolated from the rhizosphere of a healthy tomato, and the ralstonia eutropha has a weaking inhibited impact on a bacterial wilt pathogenic bacteria ralstonia eutropha (Ralstonia solanacearum) panel. However, the ralstonia eutropha possesses powerful colonization capability on the tomato rhizosphere and inside a plant, and can inhibit the infection of the ralstonia eutropha to a tomato plant. Experiments show that after the vaccine is irrigated to be applied to soil, the vaccine can breed rapidly so that an advantage group is formed in the soil, and the biocontrol efficiency can be reached up to more than 70% in the soil where the tomato bacterial wilt disease is severe. By inoculating the biocontrol bateria on the tomato plant with the root being infected by the bacterial wilt on the overground part, the further development of the disease can be effectively controlled.

Description

1. Technical field [0001] The invention discloses a microbial plant vaccine capable of preventing and controlling bacterial wilt disease of continuous cropping tomato, belonging to the agricultural intensive production technology, and is specially used for overcoming and eliminating the problem of bacterial wilt disease of continuous cropping tomato. 2. Background technology [0002] Tomato bacterial wilt is a bacterial vascular soil-borne disease caused by Ralstonia solanacearum. This disease was first discovered and reported by Simth scholars in the United States in 1906. So far, except Antarctica, other countries and regions have the existence of R. solanacearum. Ralstonia were divided into five physiological races and six biochemical races. All races had certain pathogenicity to tomato, and physiological race 1 had the strongest pathogenicity to tomato. In my country, tomato bacterial wilt mainly occurs in the warm and humid south. With the warming of the climate and t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00C12R1/01
Inventor 沈其荣韦中徐阳春黄建凤杨兴明
Owner NANJING AGRICULTURAL UNIVERSITY
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