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An antagonistic fungal strain for preventing and treating asarum leaf blight and its application

A technology to antagonize fungi and leaf blight. It can be used in applications, fungi, fungicides, etc., to solve the problems of increasing disease resistance, rampant pests, and affecting the quality and quality of medicinal materials. effect of development

Active Publication Date: 2021-11-19
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional chemical control methods have the characteristics of quick effect and wide bactericidal spectrum. However, problems such as drug residues, food safety and environmental pollution caused by irrational use of chemical pesticides are becoming more and more apparent, and even lead to rampant pests and increasing disease resistance. Biological The control can prevent and control plant diseases and insect pests under the condition of not polluting the environment, and has the advantages of safety, no residue, and strong specificity. Using biocontrol strains to prevent and control Asarum disease can help the quality of medicinal materials and increase the output of medicinal materials, which is ecological and economical. Double benefits. At present, there are few researches on the biological control of Asarum leaf blight both at home and abroad. The control of chemical pesticides on Asarum leaf blight will cause drug residues and affect the quality and quality of medicinal materials. Therefore, the present invention proposes a control method. Antagonistic fungal strains of shingle blight and applications thereof to address deficiencies in the prior art

Method used

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  • An antagonistic fungal strain for preventing and treating asarum leaf blight and its application
  • An antagonistic fungal strain for preventing and treating asarum leaf blight and its application
  • An antagonistic fungal strain for preventing and treating asarum leaf blight and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] This example proposes the isolation, screening and identification of the antagonistic fungal strain-Penicillium lavender: a variety of strains were isolated from the rhizosphere soil of healthy plants of Asarum by gradient dilution method, with Asarum leaf blight as the target bacteria, and confronted on a plate An antagonistic fungal strain numbered F1-6 was screened out by the method, and after morphological and molecular biology identification, its classification was named Penicillium janthinellum;

[0022] The specific process of isolation and screening of antagonistic fungal strains comprises the following steps:

[0023] Step 1: Use the soil dilution separation method to isolate antagonistic fungal strains, take 10g of Asarum healthy plant rhizosphere soil and add it to a 90mL sterile water conical flask, then shake the conical flask for 20min at a speed of 160r / min , mix well to make soil suspension, and then dilute the soil suspension to 10 -4 、10 -5 、10 -6 t...

Embodiment 2

[0029] according to figure 1 , 2 As shown, the present embodiment proposes the inhibitory effect of the antagonistic fungal strain-Penicillium lanuginosa on Asarum leaf blight: the antagonistic fungal strain-Penicillium lanuginosa and the pathogen of asarum leaf blight-Aceria cerevisiae are subjected to constant temperature confrontation culture , the constant temperature confrontation culture temperature is 25°C, the constant temperature culture time is 7 days, and the edge hyphae of the pathogenic bacteria cultured by the confrontation are observed;

[0030] The pathogen of asarum leaf blight - Aceria spp. (such as figure 1 shown), observed under a microscope, it was found that most of the hyphae at the edge of the pathogen colony showed fusing, constriction, increased chlamydospores, and reduced content (such as figure 2 Shown), show that the antagonistic fungal strain-Penicillium violet has obvious inhibitory effect on the growth of Asarum leaf blight pathogen-Acer sp. ...

Embodiment 3

[0032] according to image 3 , 4 As shown, the present embodiment proposes that the antifungal strain-Penicillium violet is to measure the control effect of asarum leaf blight potted plants, including the following steps:

[0033] Step 1: Divide the control effect test into 3 treatments, respectively inoculate the antagonistic fungal strain-Penicillium lavender treatment group, treat the antagonistic fungal strain-Penicillium lavender treatment group, and only inoculate the leaf blight pathogen-Acer fungus Echinococcus spray sterile water as a control group, then choose 300 leaves of Asarum with consistent growth from healthy potted Asarum, 100 for each treatment group, and respectively pierce 6 circular holes on each leaf;

[0034] Step 2: Rinse the spores of the antagonistic fungal strain-Penicillium violet with sterile water, and prepare a concentration of 1×10 8 Individual / mL spore suspension;

[0035] Step 3: The spore suspension was evenly sprayed in the vaccination t...

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Abstract

The invention discloses an antagonistic fungal strain for preventing and treating Asarum leaf blight and its application. The antagonistic fungal strain is isolated from the rhizosphere soil of healthy Asarum plants and is used to antagonize the pathogenic bacteria of Asarum leaf blight. The classification is named as : Penicillium janthinellum (Penicillium janthinellum); Preservation number: CGMCC No.18101; The present invention isolates the antagonistic fungal strain-Penicillium janthinellum that has biocontrol effect on Asarum leaf blight by isolating from Asarum healthy plant rhizosphere soil mold, and then carry out the in vivo control effect test on the antagonistic fungal strains screened out, and the preventive control effect of the antagonistic fungal strains is 70.78%, and the treatment control effect is 61.03%. It has a good effect in controlling the expansion of the disease, can induce the increase of the enzyme activity related to disease resistance in Asarum plants, and can promote the development of biological control of Asarum leaf blight.

Description

technical field [0001] The invention relates to the field of microbiology engineering and technology, in particular to an antagonistic fungal strain for preventing and treating asarum leaf blight and application thereof. Background technique [0002] Asarum, also known as fine ginseng and cigarette pot flower, is a perennial herbaceous plant of the genus Aristolochiaceae. It is an important medicinal plant. Asarum is warm in nature and pungent in taste. It has the effect of relieving cough, relieving asthma, and promoting blood circulation. It is used to treat wind-cold headache, phlegm-retention cough, joint pain, toothache, nasal sinusitis and other diseases. Asarum leaf blight is a typical multi-cycle disease. The pathogen is Mycocentrospora acerina (Hartig) Deihton. Its conidia are the main source of infection and can be transmitted through rain splash and airflow. It mainly damages leaves. The spots are nearly round, with ring patterns around the tan. Serious spot rot ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/36A01P3/00C12R1/80
CPCC12N1/14C12N1/145C12R2001/80
Inventor 郭靖刘亚苓于营王英平崔丽丽侯薇雷慧霞鲁海坤隋昕
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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