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A kind of antagonistic bacterial strain for preventing and treating asarum leaf blight and its application

A leaf blight and asarum technology, applied in bacteria, applications, fungicides, etc., can solve the problems of increasing disease resistance, drug residues, and pests rampant again, to promote development, good control, and resist the invasion of diseases. dyeing effect

Active Publication Date: 2022-01-28
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional chemical control methods have the characteristics of quick effect and wide bactericidal spectrum. However, problems such as drug residues, food safety and environmental pollution caused by irrational use of chemical pesticides are becoming more and more apparent, and even lead to rampant pests and increasing disease resistance. Biological The control can prevent and control plant diseases and insect pests under the condition of not polluting the environment, and has the advantages of safety, no residue, and strong specificity. Using biocontrol strains to prevent and control Asarum disease can help the quality of medicinal materials and increase the output of medicinal materials, which is ecological and economical. Double benefits. At present, there are few researches on the biological control of Asarum leaf blight both at home and abroad. The control of chemical pesticides on Asarum leaf blight will cause drug residues and affect the quality and quality of medicinal materials. Therefore, the present invention proposes a control method. Antagonistic bacterial strains of shingle blight and applications thereof to address deficiencies in the prior art

Method used

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  • A kind of antagonistic bacterial strain for preventing and treating asarum leaf blight and its application
  • A kind of antagonistic bacterial strain for preventing and treating asarum leaf blight and its application
  • A kind of antagonistic bacterial strain for preventing and treating asarum leaf blight and its application

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Effect test

Embodiment 1

[0020] This example proposes the isolation, screening and identification of antagonistic bacterial strains-Brevibacillus spp.: from the rhizosphere soil of healthy Asarum plants, a variety of strains were isolated by the gradient dilution method, with Leaf Blight of Asarum as the target bacteria, using a flat plate An antagonistic bacterial strain numbered S2-31 was screened out by the confrontation method, which was identified by morphology and molecular biology and named as Brevibacillus laterosporus;

[0021] The specific process of isolation and screening of antagonistic bacterial strains comprises the following steps:

[0022] Step 1: Use the soil dilution separation method to isolate antagonistic bacterial strains, take 10g of Asarum healthy plant rhizosphere soil and add it to a triangular flask with 90mL of sterile water, then shake the flask for 20min at a speed of 160r / min , mix well to make soil suspension, and then dilute the soil suspension to 10 -4 、10 -5 、10 ...

Embodiment 2

[0028] according to figure 1 , 2 As shown, the present embodiment proposes that the inhibitory effect of the antagonistic bacterial strain-Brevibacillus spp. Carry out constant temperature confrontation culture, constant temperature confrontation culture temperature is 25 ℃, constant temperature culture time is 7 days, and observe the edge mycelia morphology of the pathogenic bacteria cultured by confrontation;

[0029] After being treated with the antagonistic bacterial strain-Brevibacillus spp., the pathogen of asarum leaf blight-Acerella aceriformis (such as figure 1 Shown), observed under a microscope, it was found that most of the hyphae at the edge of the pathogenic bacteria colony were broken, twisted, constricted, and chlamydospores increased (such as figure 2 Shown), it shows that the antagonistic bacterial strain-Brevibacillus spp. has obvious inhibitory effect on the growth of Asarum leaf blight pathogen-Acer sporosum.

Embodiment 3

[0031] according to image 3 , 4 As shown, the present embodiment proposes the determination of the antagonism effect of the antagonistic bacterial strain - Brevibacillus spp. on Asarum leaf blight potted plant living body, comprising the following steps:

[0032] Step 1: Inoculate the antagonistic bacterial strain, Brevibacillus serospora, which has been activated for 24 hours, into 150 mL of sterilized LB liquid medium, and shake it for 48 hours at a speed of 180 r / min and a temperature of 32 ° C to obtain bacterial fermentation liquid, and then adjust the cell fermentation liquid to a concentration of 1×10 8 Individual / mL bacterial suspension;

[0033] Step 2: The determination of the antagonistic effect is divided into two treatments, respectively inoculating the antagonistic bacterial strain-Brevibacillus spp. and then inoculating the treatment group with the pathogenic bacteria of leaf blight and inoculating only the pathogenic bacteria of leaf blight-Acer sporogenes a...

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Abstract

The invention discloses an antagonistic bacterial strain for preventing and treating Asarum leaf blight and its application. The antagonistic bacterial strain is isolated from the rhizosphere soil of a healthy Asarum plant and is used to antagonize the pathogenic bacteria of Asarum leaf blight; the classification is named as : Brevibacillus laterosporus (Brevibacillus laterosporus); Preservation number: CGMCC No.18015; The method of the present invention is by separating the antagonistic bacterial strain that has biocontrol effect to Asarum leaf blight from Asarum healthy plant rhizosphere soil. Bacillus spores, then carry out antibacterial effect test and in vivo antagonistic effect test in the plate, draw the control effect of antagonistic bacterial strain Brevibacillus spp. The occurrence of leaf blight has a good control effect, and can induce the increase of the activity of enzymes related to disease resistance in Asarum plants, thereby resisting the infection of the disease.

Description

technical field [0001] The invention relates to the field of microbiology engineering and technology, in particular to an antagonistic bacterial strain for preventing and treating asarum leaf blight and its application. Background technique [0002] Asarum, also known as fine ginseng and cigarette pot flower, is a perennial herbaceous plant of the genus Aristolochiaceae. It is an important medicinal plant. Asarum is warm in nature and pungent in taste. It has the effect of relieving cough, relieving asthma, and promoting blood circulation. It is used to treat wind-cold headache, phlegm-retention cough, joint pain, toothache, nasal sinusitis and other diseases. Asarum leaf blight is a typical multi-cycle disease. The pathogen is Mycocentrospora acerina (Hartig) Deihton. Its conidia are the main source of infection and can be transmitted through rain splash and airflow. It mainly damages leaves. The spots are nearly round, with ring patterns around the tan. Serious spot rot a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P3/00C12R1/01
CPCA01N63/00C12R2001/01C12N1/205
Inventor 郭靖刘亚苓于营王英平崔丽丽侯薇雷慧霞鲁海坤隋昕
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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