Fruit and vegetables antiscaling agent containing regrouped chitinase

Abstract

A fruit and vegetable antiseptic and fresh-keeping agent containing recombinant chitinase. The main active ingredient of the fruit and vegetable antiseptic and antistaling agent is recombinant chitinase, which is to transfer the Class I alkaline chitinase gene into Pichia pastoris by means of molecular biology. , and then induce its secretion in the fermentation broth with methanol, and then extract it from the supernatant after fermentation. In addition, it also contains ionic buffer to maintain its activity, oligosaccharides with synergistic antibacterial effect, etc. As an antiseptic and preservative for fruits and vegetables, it can induce the disease-resistant response of fresh fruits and vegetables before harvesting; it can resist the infection of pathogenic bacteria or kill the new mycelium when used after harvesting, and is used to prevent gray mold and root of fruits and vegetables after harvesting. Corrosion caused by pathogenic fungi such as mildew and Aspergillus. The invention belongs to the application of biological technology in the prevention and control of post-harvest disease and rot of fruits and vegetables. The production process is mature, and the cost of production and use is low. It provides low-cost, high-efficiency, non-toxic and pollution-free biological induced disease resistance for anti-corrosion and preservation of post-harvest fruits and vegetables. Fresh-keeping agent with broad application prospects.

Description

technical field [0001] The invention belongs to the product of the combination of biotechnology and agricultural product preservation, in particular to a fruit and vegetable preservative and preservative containing recombinant chitinase, its production process and its application in fresh fruit and vegetable preservation. Background technique [0002] Fungal diseases are the main cause of rotten vegetables after harvest, and the resulting losses are huge. In order to effectively prevent and control fungal diseases, relevant scientific researchers have paid a lot of hard work in the past century, and some effective results have also been obtained, such as using engineering bacteria to cultivate disease-resistant varieties. This approach is due to influencing factors. Complex, antifungal effects vary and have not been commercialized. As for the common fungal diseases of fruits and vegetables after harvesting, chemical fungicides are still the main ones at home and abroad. The...

AI Technical Summary

Problems solved by technology

So so far, there is no effective, low-cost and large-scale production of recombinant chitinase and a fruit and vegetable antiseptic preservative used in post-harvest storage and preservation of fruits and vegetables.

Images