Application method for applying vanilline under soil conditions to prevent and treat meloidogyne incognita chitwood
A technology of root-knot nematode incognita and its application method, which is applied in the field of plant protection using vanillin, can solve the problems that the effect of vanillin on nematode control is not clear, and achieve the effect of inhibiting infection and reproduction
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Embodiment 1
[0029] Embodiment 1 is directly applied to the control effect of vanillin on root-knot nematode incognita
[0030] In the present embodiment, the cultivation and collection methods of root-knot nematode incognita are as follows:
[0031] Plant planting: Soak Jiafen No. 18 tomato seeds in 15% (V / V) hydrogen peroxide solution for 15 minutes to sterilize, wash with water, spread the seeds evenly in a petri dish covered with two layers of moist filter paper, and keep away from light The treatment was placed in a constant temperature culture room. After germination, it is sown on a seedling board containing vermiculite. After the seedlings grow to three leaves and one heart, they are transplanted into plastic pots filled with sterilized soil. Nematodes were inoculated 10 days after transplanting.
[0032] Nematode inoculation method: uniformly punch four 2-3cm deep holes at about 1cm at the base of the tomato stem, inject the suspension containing the second instar larvae of Mel...
Embodiment 2
[0037] Except that the concentration of vanillin prepared is 0.2mmol / kg·drysoil, other operations are the same as in Example 1.
Embodiment 3
[0039] Except that the concentration of vanillin prepared is 0.4mmol / kg·drysoil, other operations are the same as in Example 1.
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