Peach-auxin-primary-response-factor Ppa011935m gene and application thereof

Abstract

The invention relates to application of peach-auxin-primary-response-factor Ppa011935m gene to tomatoes. The Ppa011935m gene is obtained after cloning from peach fruits, and is transferred into the tomatoes and applied, and the result shows that the plant type of the tomatoes can be changed through Ppa011935m gene overexpression, the internode of the tomatoes extends, the plant height of the tomatoes is increased, the fruit shape of the tomatoes is changed, acuminate fruits are formed, the quantity of seeds in fruits is decreased, and even seedless fruits are formed. Meanwhile, Ppa011935m transgene promotes releasing of fruit ethylene, and the fruits are matured in advance. The invention provides a method capable of changing the plant type of the tomatoes, the seed quantity and promoting fruit maturation, and the method includes the step that the Ppa011935m gene is introduced into the tomatoes, and correlated characters of nutrition growth and reproductive growth of the tomatoes can beeffectively changed.

Description

technical field [0001] The invention relates to a peach auxin primary response factor gene, in particular to the application of the peach auxin primary response factor Ppa011935m gene in changing tomato plant type, fruit shape, root system and fruit maturity period. Background technique [0002] Auxin plays a very important role in the growth and ripening of peach fruit. High concentrations of auxin are necessary for the synthesis of ethylene in climacteric fruits, which can cause softening of solute peaches in late ripening. In contrast, due to the low concentration of auxin produced by hard peach fruit when it matures, almost no ethylene is produced, and the fruit cannot be softened. Exogenous naphthalene acetic acid treatment can cause ripening and softening of hard peach fruit, which proves the role of auxin in this process. Auxin rapidly induces the expression of a series of auxin-responsive genes by regulating the protein level of Aux / IAA (auxin / indole-3-acetic acid)...

AI Technical Summary

Problems solved by technology

Chrysanthemum CmIAA1 gene transformed Arabidopsis, resulting in slower growth and development of transgenic Arabidopsis than wild type, and its tap root was shorter than wild type; poplar PtrIAA14.1 transformed Arabidopsis, transgenic plants showed a series of auxin-related phenotypes , including leaf roll down, increased inflorescence stem branching, and reduced seed setting

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