Peach-auxin-primary-response-factor Ppa011935m gene and application thereof

A response factor, auxin technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problems of slow growth and development of transgenic Arabidopsis, reduced seed setting rate, short main root and other problems, and achieves increased gene expression and ethylene release. The effect of increasing the amount and promoting rapid maturity

Active Publication Date: 2018-09-07
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chrysanthemum CmIAA1 gene transformed Arabidopsis, resulting in slower growth and development of transgenic Arabidopsis than wild type, and its tap root was shorter than wild type; poplar P

Method used

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  • Peach-auxin-primary-response-factor Ppa011935m gene and application thereof
  • Peach-auxin-primary-response-factor Ppa011935m gene and application thereof
  • Peach-auxin-primary-response-factor Ppa011935m gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039]Example 1 Isolation and Identification of Ppa011935m Gene

[0040] Test materials: solute-type peach variety CN13 and hard-type peach variety CN16, Micro-Tom tomato, pCambia1380 plasmid, Agrobacterium GV3101.

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Abstract

The invention relates to application of peach-auxin-primary-response-factor Ppa011935m gene to tomatoes. The Ppa011935m gene is obtained after cloning from peach fruits, and is transferred into the tomatoes and applied, and the result shows that the plant type of the tomatoes can be changed through Ppa011935m gene overexpression, the internode of the tomatoes extends, the plant height of the tomatoes is increased, the fruit shape of the tomatoes is changed, acuminate fruits are formed, the quantity of seeds in fruits is decreased, and even seedless fruits are formed. Meanwhile, Ppa011935m transgene promotes releasing of fruit ethylene, and the fruits are matured in advance. The invention provides a method capable of changing the plant type of the tomatoes, the seed quantity and promoting fruit maturation, and the method includes the step that the Ppa011935m gene is introduced into the tomatoes, and correlated characters of nutrition growth and reproductive growth of the tomatoes can beeffectively changed.

Description

technical field [0001] The invention relates to a peach auxin primary response factor gene, in particular to the application of the peach auxin primary response factor Ppa011935m gene in changing tomato plant type, fruit shape, root system and fruit maturity period. Background technique [0002] Auxin plays a very important role in the growth and ripening of peach fruit. High concentrations of auxin are necessary for the synthesis of ethylene in climacteric fruits, which can cause softening of solute peaches in late ripening. In contrast, due to the low concentration of auxin produced by hard peach fruit when it matures, almost no ethylene is produced, and the fruit cannot be softened. Exogenous naphthalene acetic acid treatment can cause ripening and softening of hard peach fruit, which proves the role of auxin in this process. Auxin rapidly induces the expression of a series of auxin-responsive genes by regulating the protein level of Aux / IAA (auxin / indole-3-acetic acid)...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/00A01H6/82
CPCC07K14/415C12N15/8261
Inventor 曾文芳王志强丁义峰牛良鲁振华崔国朝潘磊高昂
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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