Heavy metal resistant bacteria and application thereof

A heavy metal and resistance technology, applied in the field of microbial remediation, can solve the urgent problems of copper-contaminated soil remediation, and achieve the effect of low cost and reduced toxicity

Active Publication Date: 2020-04-17
AGRI ECOLOGY INST FUJIAN ACADEMY OF AGRI SCI +1
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Problems solved by technology

Therefore, the remediation of copper-contaminated soil is urgent

Method used

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  • Heavy metal resistant bacteria and application thereof
  • Heavy metal resistant bacteria and application thereof
  • Heavy metal resistant bacteria and application thereof

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Embodiment Construction

[0014] The invention provides a strain of Ralstonia sp. RalstoniaBcul-1, the Ralstonia sp. is isolated and purified from the farmland soil around Fujian Youxi lead-zinc mining area, and has the characteristics of heavy metal-resistant microorganisms bacterial strains.

[0015] The strain screening steps are as follows:

[0016] Step 1: Add 10g of fresh soil into 90mL sterile water with glass beads, 150r min -1 After shaking for 10 minutes, let stand for 5 minutes and dilute to prepare 10 -2 bacterial suspension;

[0017] Step 2: Take 5mL of 10 -2 The bacterial suspension was inoculated into 50ml Cu 2+ The concentration is 200mg·L -1 In the beef extract peptone liquid medium, under sterile conditions 37 ℃ 150r min -1 Shake culture for 24h;

[0018] Step 3: above-mentioned culture fluid is transferred into fresh culture fluid (Cu 2+ The concentration is 400mg·L -1 150r·min in ) -1 Continue shaking culture for one cycle, and then transfer twice to Cu in liquid medium 2...

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Abstract

The invention belongs to the technical field of microbial remediation, and specifically relates to heavy metal resistant bacteria and an application thereof. The heavy metal resistant bacteria provided by the invention is Ralstonia Bcul-1, which is preserved in China General Microbiological Culture Collection Center (CGMCC), Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, West Beichen Road, Chaoyang District, Beijing on April 12, 2019, with an accession number being CGMCC NO. 17565. The heavy metal resistant bacteria provided by the invention are used for biological remediation of a heavy metal contaminated water body or heavy metal contaminated soil. According to the invention, microorganisms in the soil environment are used for passivating and adsorbing heavy metalpollutants to reduce toxicity of heavy metals in soil, so the advantages of low cost, high efficiency, no secondary pollution and the like are achieved.

Description

【Technical field】 [0001] The invention belongs to the technical field of microbial remediation, and in particular relates to heavy metal resistant bacteria and applications thereof. 【Background technique】 [0002] In recent years, the problem of heavy metal pollution in farmland has become a major agricultural eco-environmental issue of widespread concern in my country, posing a serious threat to the sustainable development of modern agriculture and social economy, the safety of agricultural ecological environment and the quality and safety of agricultural products. The Ministry of Agriculture conducted a soil survey of 320 seriously polluted areas in 24 provinces and cities across the country and found that the rate of excessive heavy metals in the soil accounted for 80% of the polluted soil and crops. From 2005 to 2013, the Ministry of Environmental Protection, together with the Ministry of Land and Resources, carried out the first national soil unmanned survey, and the re...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/01C02F101/20
CPCB09C1/10C02F3/34C02F2101/20C12R2001/01C12N1/205
Inventor 王义祥黄家庆陈睿李艳春叶菁林怡
Owner AGRI ECOLOGY INST FUJIAN ACADEMY OF AGRI SCI
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