Trichoderma asperellum TD3104 and application thereof in preparation of microbial agent for inhibiting plant pathogenic bacteria

A technology for plant pathogenic bacteria and Trichoderma, which is applied in the directions of plant growth regulators, applications, fungicides, etc., can solve the problems of environmental pollution and serious diseases of apple branches and stems year by year, and achieves good inhibitory effect, significant economic and social benefits, The effect of preventing fruit contamination

Active Publication Date: 2017-11-24
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the problems of environmental pollution caused by chemical pesticide control in the prior art, severe tobacco continuous cropping diseases and serious apple branch ...

Method used

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  • Trichoderma asperellum TD3104 and application thereof in preparation of microbial agent for inhibiting plant pathogenic bacteria
  • Trichoderma asperellum TD3104 and application thereof in preparation of microbial agent for inhibiting plant pathogenic bacteria
  • Trichoderma asperellum TD3104 and application thereof in preparation of microbial agent for inhibiting plant pathogenic bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: Isolation of antagonistic bacteria

[0028] The corn rhizosphere soil is separated by the soil dilution separation method. The Trichoderma aculeatus TD3104 of the present invention is isolated from this soil. By purifying the isolated single spore, it confronts the common tobacco pathogenic bacteria at two points on the PDA plate. Inoculate, carry out plate antagonistic test, and finally screen to obtain.

Embodiment 2

[0029] Embodiment 2, identification of antagonistic bacteria

[0030] 1. Morphological identification of antagonistic bacteria

[0031] The medium used in the experiment is as follows:

[0032] (1) Potato-dextrose agar (PDA): Take 200g of peeled potatoes, cut into small pieces, add 1000mL of water, boil for 10min, filter with gauze, add 20g of agar and 20g of glucose to the filtrate, heat it to melt, and finally make it to 1000mL , sterilized at 121°C for 20 minutes.

[0033] (2) LB medium: tryptone 10g, yeast extract 5g, NaCl 5g, distilled water 1000mL, pH 7.4, sterilized at 121°C for 20min.

[0034] (3) LB solid medium: 20 g / L agar was added to the LB medium.

[0035] (4) Oat culture medium: 30 grams of oatmeal, 20 grams of agar, 1000 mL of water, sterilized at 121°C for 20 minutes.

[0036] (5) Potato-glucose (PD): Agar was removed from PDA.

[0037] The screened strains were inoculated on PDA plates, placed in an incubator with constant temperature and humidity at 28°...

Embodiment 3

[0044] The antibacterial spectrum of embodiment 3, Trichoderma aculeatus TD3104

[0045] 1. Antagonism to pathogenic fungi

[0046] Inoculate Trichoderma aculeatus TD3104 with tobacco black shank, Rhizoctonia solani, Fusarium tobacco root rot, tobacco red spot, apple rot and apple ring spot respectively on the PDA plate by two-point confrontation method At the same time, the PDA plate not inoculated with each pathogen of Trichoderma aculeatus TD3104 was used as a control. Place them in a constant temperature and humidity incubator at 28°C for cultivation. Observe the growth of pathogenic colonies 3 days after inoculation. When the pathogens come into contact with antagonistic bacteria, measure the radius of the pathogenic colonies and calculate the antibacterial rate.

[0047] Bacterial inhibition rate (%)=(control colony radius-treated colony radius) / control colony radius×100.

[0048] Table 1 Antagonism of Trichoderma aculeatus TD3104 to several fungal pathogens

[0049] ...

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Abstract

The invention provides Trichoderma asperellum TD3104 and application thereof in preparation of microbial agent for inhibiting plant pathogenic bacteria. The preservation number of the Trichoderma asperellum TD3104 is CGMCC 13161. The invention further provides application of the Trichoderma asperellum in the controlling of the plant pathogenic bacteria of tobacco and apples. The antagonistic Trichoderma asperellum can effectively inhibit the growth of Phytophthora parasitica var. nicotianae, ralstonia solannacearum, rhizoctonia solani, tobacco root rot pathogenic fusarium, alternaria alternata and the pathogenic bacteria of apple rot and apple ring rot, can effectively control related diseases, can reduce the use amount of chemical pesticides and accumulation of the chemical pesticides in soil, is beneficial to the increasing of quality and yield of the economic crops of China and is evident in economic and social benefits.

Description

technical field [0001] The invention belongs to the field of microorganisms and biological control, and in particular relates to Trichoderma aculeatus TD3104 and its application in the preparation of bacterial agents for inhibiting plant pathogenic bacteria. Background technique [0002] Tobacco and apple are both important economic crops in my country, and their yield and quality are related to the vital interests of growers. High-quality tobacco has certain requirements on the soil and environmental conditions in the producing area, so continuous tobacco cropping is common. After continuous cropping, the disease aggravated and the yield decreased. In recent years, apple branch diseases have occurred seriously, and the joint infection of apple rot and apple ring spot has increased year by year. At present, the general method to control tobacco and apple diseases is to use chemical pesticides; however, long-term use of chemical pesticides will lead to resistance of pathoge...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
CPCA01N63/30C12N1/145C12R2001/885
Inventor 李雅华刘新咸洪泉赵方贵
Owner QINGDAO AGRI UNIV
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